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Developmental competence of Dromedary camel (Camelus dromedarius) oocytes selected using brilliant cresyl blue staining

Published online by Cambridge University Press:  11 July 2017

Mohamed Fathi ,
Mohamed Ashry ,
Ali Salama  and
Magdy R. Badr
Mohamed Fathi
Affiliation:
Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Mohamed Ashry*
Affiliation:
B275 Anthony Hall, department of Animal Science, Michigan State University, East Lansing, MI 48824, USA. Laboratory of Mammalian Reproductive Biology, Department of Animal Science, Michigan State University, USA.
Ali Salama
Affiliation:
Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Magdy R. Badr
Affiliation:
Department of Artificial Insemination and Embryo Transfer, Animal Reproduction Research Institute, Agriculture Research Centre, Giza, Egypt.
*
All correspondence to: M. Ashry: B275 Anthony Hall, department of Animal Science, Michigan State University, East Lansing, MI 48824, USA. E-mail: [email protected]
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Summary

The objectives of the present studies were to investigate the developmental capacity of dromedary camel oocytes selected by brilliant cresyl blue (BCB) staining and to investigate the expression of select transcripts in germinal vesicle (GV) stage oocytes. These transcripts included BMP15 and GDF9 as important transcripts for folliculogenesis and oocyte development, Zar1 and Mater as maternal transcripts required for embryonic development, Cyclin B1 and CDK1 as cell cycle regulators and Oct4 and STAT3 as transcription factors. Dromedary camel oocytes were retrieved from ovaries collected at a local slaughterhouse. After exposure to BCB staining, cumulus–oocyte complexes (COCs) from BCB+, BCB− and control (selected based on morphological criteria) groups were subjected to in vitro maturation, in vitro fertilization and in vitro culture. For gene expression studies, after BCB staining cumulus cells were stripped off and the completely denuded GV stage oocytes were used for RT-PCR analysis of selected transcripts. BCB+ oocytes showed higher maturation, and fertilization rates compared with BCB− and control groups. Indices of early embryonic development, namely, cleavage at 48 hours post insemination (hpi), and development to morula at day 5 and day 7 blastocyst rates were also significantly higher in the BCB+ group. RT-PCR revealed a higher expression of BMP15, GDF9, Zar1, Mater, Cyclin B1, CDK1, OCT4 and STAT3 in good quality oocytes that stained positively for BCB (BCB+). Collectively, results provide novel information about the use of BCB screening for selecting good quality oocytes to improve in vitro embryo production in the dromedary camel.

Keywords

BCBBrilliant cresyl blueDromedary camelOocytesGene expression
Type
Research Article
Information
Zygote , Volume 25 , Issue 4 , August 2017 , pp. 529 - 536
Copyright
Copyright © Cambridge University Press 2017 

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Footnotes

5

These authors contributed equally to this work.

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