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Determination of the ideal volume of activating solution and the optimal ratio of spermatozoa per oocyte for Prochilodus brevis fertilization

Published online by Cambridge University Press:  18 November 2019

Mayara Setúbal Oliveira-Araújo Open the ORCID record for Mayara Setúbal Oliveira-Araújo [Opens in a new window] ,
Júlia Trugilio Lopes ,
Larissa Teixeira Nunes ,
Priscila Silva de Almeida-Monteiro ,
Renata Vieira do Nascimento ,
Vanessa Alves Pereira ,
Yasmim Maia Ferreira ,
Raphael William Ponte Neres ,
Assis Rubens Montenegro  and
Liliane Veras Leite-Castro
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Mayara Setúbal Oliveira-Araújo*
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Júlia Trugilio Lopes
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Larissa Teixeira Nunes
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Priscila Silva de Almeida-Monteiro
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Renata Vieira do Nascimento
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Vanessa Alves Pereira
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Yasmim Maia Ferreira
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Raphael William Ponte Neres
Affiliation:
Faculty of Veterinary Medicine, State University of Ceará, UECE, Fortaleza, CE, Brazil
Assis Rubens Montenegro
Affiliation:
Postgraduate Programme in Veterinary Science, State University of Ceará, UECE, Fortaleza, CE, Brazil
Liliane Veras Leite-Castro
Affiliation:
Dept of Biology, Federal Institute of Education, Science and Technology of Ceará, IFCE, Acopiara, CE, Brazil
Carminda Sandra Brito Salmito-Vanderley
Affiliation:
Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, Brazil
*
Author for correspondence: Mayara Setúbal Oliveira-Araújo, Faculty of Veterinary Medicine, State University of Ceará, UECE, Fortaleza, CE, 60740-000, Brazil. Tel:/Fax: +85 3101 9851. E-mail: [email protected]
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Summary

Prochilodus brevis is a rheophilic species with a threatened natural population that promotes studies aimed at optimizing reproduction in captivity. The correct quantity of inseminating dose and activating solution volume significantly improves fertilization rates, thereby increasing productivity in captivity. The objective of this study was to determine the proportion of sperm per oocyte and the ideal volume of activating solution to be used in the assisted fertilization of P. brevis. Gametes were collected and fertilization performed in two steps. In step 1, the ideal proportion of spermatozoa was determined based on the fertilization rate:oocyte by testing six doses of semen: D1 = 30 × 103, D2 = 150 × 103, D3 = 300 × 103, D4 = 3 × 106, D5 = 5 × 106, and D6 = 10 × 106. In step 2, the fertilization and hatching rates were evaluated using different volumes of activating solution (V1 – 25 ml, V2 – 50 ml, V3 – 75 ml,V4 – 100 ml, V5 – 125 ml, and V6 – 150 ml). A linear regression equation was estimated from steps 1 and 2. The Student–Newman–Keuls test was used to compare the means. In step 1, the percentage of fertilization increased linearly, reaching a plateau of 51.69%. In step 2, the best fertilization rates were obtained with an estimated ideal volume of 75.64 ml per 2 ml of oocytes. Therefore, the proportion of 928,410.29 sperm:oocyte, associated with the volume of 75.64 ml of water per 2 ml of oocytes, provided the maximum reproductive performance for P. brevis.

Keywords

Assisted reproductionBrazilian bocachicoCharacidaeFreshwater fishInseminating dose
Type
Research Article
Information
Zygote , Volume 28 , Issue 2 , April 2020 , pp. 103 - 108
Copyright
© Cambridge University Press 2019

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References

Adames, MS, Toledo, CPR, Neumann, G, Buzzi, AH, Buratto, CN, Piana, PA and Bombardelli, RA (2015) Optimization of the sperm: oocyte ratio and sperm economy in the artificial reproduction of Rhamdia quelen using fructose as a sperm motility modulator. Anim Reprod Sci 161, 119–28.CrossRefGoogle ScholarPubMed
Alavi, SMH, Rodina, M, Policar, T, Kozak, P, Psenicka, M and Linhart, O (2007) Semen of Perca fluviatilis L.: sperm volume and density, seminal plasma indices and effects of dilution ratio, ions and osmolality on sperm motility, Theriogenology 68, 276–83.CrossRefGoogle ScholarPubMed
Alves, DEO, Silva, MDFM, Molina, WF, Costa, SAGL and Nascimento, RSS (2016) Desenvolvimento ontogenético inicial de Prochilodus brevis (Steindachner, 1875) (Characiformes). Bio Amaz 6, 70–5.CrossRefGoogle Scholar
Anuário Brasileiro da Piscicultura Peixe Br 2018 (2018) São Paulo: Associação Brasileira da Piscicultura. 140 pp.Google Scholar
Araújo, SA and Gurgel, HCB (2002) Aspectos da biologia de Prochilodus cearenses (Steindachner, 1911) (Characiformes, Prochilodontidae) no açude Itans/Caicó, Rio Grande do Norte. Rev Bras Zool 4, 85–96.Google Scholar
Baggio, DM, Sanches, EA, Sykora, RM, Bombardelli, RA, Souza, BE and Vidal, E (2007) Relação espermatozóide:ovócito na fertilização de ovócitos do cascudo-preto (Rhinelepis aspera) In: Congresso Brasileiro de Produção de Peixes Nativos de Água Doce, Embrapa, Dourados. Anais Dourados: CBPPNAD. Resumo.Google Scholar
Billard, R, Cosson, MP, Perchec, G and Linhart, O (1995) Biology of sperm and artificial reproduction in carp Aquaculture 129, 95–112.CrossRefGoogle Scholar
Bombardelli, RA, Morschbacher, EF, Campagnolo, R, Sanches, EA and Syperreck, MA (2006) Insemination dose for artificial fertilization of grey jundia oocytes, Rhamdia quelen (Quoy and Gaimardm, 1824). Rev Bras Zool 35, 1251–7.CrossRefGoogle Scholar
Borges, A, Siqueira, DR, Jurinitz, DF, Zanini, R, Amaral, F, Grillo, ML, Oberst, ER and Wassermann, GF (2005) Biochemical composition of seminal plasma and annual variations in semen characteristics of jundia’ Rhamdia quelen (Quoy and Gaimard, Pimelodidae). Fish Physiol Biochem 31, 45–53.CrossRefGoogle Scholar
Carneiro, PCF (2007) Tecnologias de produção e armazenamento de sêmen de peixe. Rev Bras Rep Ani 31, 361–6.Google Scholar
Castro, RMC (1991) Sistemática e distribuição geográfica da família Prochilodontidae (Ostariophysi, Characiformes). In: Encontro Brasileiro de Ictiologia, IX. Maringá, Pr, Resumos. Maringá, SBI/NUPELIA, 128 pp.Google Scholar
Denniston, RS, Michelet, S and Godke, RA (2000) Principles of Cryopreservation In Tiersch, TR and Mazik, PM (eds). Cryopreservation in aquatic species. Morgantown, T. Word. Aquac Soc pp. 59–74.Google Scholar
Dourado, OF (1981) Principais peixes e crustáceos dos açudes controlados pelo DNOCS. Fortaleza, Convênio SUDENE/DNOCS. 40 pp.Google Scholar
Felizardo, VDO, Murgas, LDS, Drumond, MM and Aquino Silva, J (2010) Dose inseminante utilizada na fertilização artificial de ovócito de piracanjuba (Brycon orbignyanus). Ceres 57, n. 5.Google Scholar
Food and Agriculture Organization of The United Nations (FAO) (2016) The State of World Fisheries and Aquaculture – Contributing to food security and nutrition for all. Roma: FAO. 200 pp.Google Scholar
García, AVJ, Espinosa, JA, Martínez, JG and Pardo Carrasco, SC (2015) Insemination of bocachico fish (Prochilodus magdalenae) with fresh or cryopreserved semen: effect of spermatozoa/oocyte ratio. Rev Col Cie Pec 28, 347–55.Google Scholar
Lahnsteiner, F (2000) Semen cryopreservation in the salmonidae and in the northern pike. Aquacult Res 31, 245–58.CrossRefGoogle Scholar
Leite, LV, Melo, MAP, Oliveira, FCE, Pinheiro, JPS, Campello, CC, Nunes, JF and Salmito-Vanderley, CSB (2013) Determinação da dose inseminante e embriogênese na fertilização artificial de tambaqui (Colossoma macropomum). Arq Bras Med Vet Zoot 65, 421–9.CrossRefGoogle Scholar
Lizama, MAP (2000) Estimativa dos parâmetros de crescimento, recrutamento e mortalidade de Prochilodus lineatus da planície de inundação do Alto Rio Paraná. Boletim do Instituto de Pesca, São Paulo 26, 121–8.Google Scholar
Martínez-Páramo, S, Horváth, A, Labbé, C, Zhang, T, Robles, VHerráez, P, Suquet, M, Adams, S, Viveiros, A, Tiersch, TR and Cabrita, E (2017) Cryobanking of aquatic species. Aquaculture 472, 156–77.CrossRefGoogle ScholarPubMed
Murgas, LDS, Miliorini, AB, Freitas, RTF and Pereira, GJM (2007) Criopreservação do sêmen de curimba (Prochilodus lineatus) mediante adição de diferentes diluidores, ativadores e crioprotetores. Rev Bras Zoot 36, 526–31.CrossRefGoogle Scholar
Nascimento, RV, Leite-Castro, LV, Montenegro, AR, Oliveira-Araújo, MS, Lopes, JT, Almeida-Monteiro, PS and Salmito-Vanderley, CSB (2017) Influence of cooling time and diluents on the freezability of Prochilodus brevis semen. Acta Sci Vet 45, 1–9.Google Scholar
Nunes, LT, Oliveira, MSLopes, JTSouza, MEM, Pinheiro, RRR, Campello, CC and Salmito-Vanderley, CSB (2016) Cryopreservation of Prochilodus brevis semen: freezing media and thawing rates. Semina: Ciê Agrá 37, 1643–54.Google Scholar
Oliveira-Silva, L, Ramos, TPA, Carvalho-Rocha, YGP, Viana, KMP, Avellar, RC and Ramos, RTC (2016) Ichthyofauna of the Mamanguape river basin, Northeastern, Brazil. Bio Neot 18, 1–11.Google Scholar
Romagosa, E (2006) Biologia reprodutiva e fisiologia de peixes em confinamento: o cachara Pseudoplatystoma fasciatum como modelo. In Cyrino, JEP and Urbinati, EC (eds). Tópicos Especiais em Biologia Aquática e Aquicultura. AQUABIO, Jaboticabal, pp. 108–16.Google Scholar
Sanches, EA, Bombardelli, RA, Baggio, DM and Souza, BED (2009) Dose inseminante para fertilização artificial de ovócitos de dourado. Rev Bras Zoot 2091–8.CrossRefGoogle Scholar
Sanches, EA, Baggio, DM, Piana, PA, Souza, BED and Bombardelli, R (2011) A Artificial fertilization of oocytes and sperm activation in pacu: effects of the spermatozoa: oocyte ratio, water volume and in natura semen preservation. Rev Bras Zoot 40, 1–6.CrossRefGoogle Scholar
Sanches, EA, Caneppele, D, Okawara, RY, Damasceno, DZ, Bombardelli, RA and Romagosa, E (2016) Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish. Neot. Ichthy. 14, e140158.Google Scholar
Shimoda, E, Andrade, DR, Júnior, V, Godinho, HP and Yasui, GS (2007) Determination of the optimum ratio of spermatozoa per oocyte of the piabanha Brycon insignis. Arq Bras Med Vet Zoot 59, 877–82.CrossRefGoogle Scholar
Souza, BE (2007) Fertilização artificial de ovócitos de curimbatá, Prochilodus lineatus. 63 f. Dissertação (Mestrado) - Curso de Aquicultura, Centro de Aquicultura, Universidade Estadual Paulista, Jaboticabal, 2007. Available at: https://repositorio.unesp.br/bitstream/handle/11449/86686/souza_be_me_jabo.pdf?sequence=1andisAllowed=y. Accessed 15 November 2018.Google Scholar
Streit, DP Jr, Moraes, GV, Ribeiro, RP, Povh, JA, Souza, ED and Oliveira, CAL (2004) Avaliação de diferentes técnicas para coloração de sêmen de peixes. Arq Ciê Vet Zool UNIPAR 7, 157–62.Google Scholar
Suquet, M, Billard, R, Cosson, J, Normant, Y and Fauvel, C (1995) Artificial insemination in turbo (Scophthalmus maximus): determination of the optimal sperm to egg ratio and time of gamete contact. Aquaculture 133, 83–90.CrossRefGoogle Scholar
Vazzoler, AEAM (1996) Biologia da reprodução de peixes teleósteos: teoria e prática. Maringá: NUPELIA. 169 pp.Google Scholar
Woynarovich, E and Horváth, L (1983) A propagação artificial de peixes de águas tropicais. Brasília, FAO/ CODEVASF/CNPq. 220 pp.Google Scholar
Zaniboni-Filho, E and Weingartner, M (2007) Técnicas de indução da reprodução de peixes migradores. Rev Bras Rep Ani 31, 367–73.Google Scholar