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Cytological studies on induced mitogynogenesis in Japanese flounder Paralichthys olivaceus (Temminck et Schlegel)

Published online by Cambridge University Press:  21 January 2016

Jilun Hou ,
Guixing Wang ,
Xiaoyan Zhang  and
Haijin Liu
Jilun Hou*
Affiliation:
Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
Guixing Wang
Affiliation:
Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
Xiaoyan Zhang
Affiliation:
Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
Haijin Liu
Affiliation:
Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing, 100141, China.
*
All correspondence to: Jilun Hou. Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China. Tel.: +86 335 5922354. Fax: +86 335 4260826. E-mail: [email protected]
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Summary

The effect of hydrostatic pressure treatment on the induction of mitogynogenesis in the eggs of Japanese flounder Paralichthys olivaceus (Temminck et Schlegel) by using heterospecific sperm were studied. Before treatment, the eggs were at metaphase of the first mitosis. The spindle was disassembled by the treatment and then resembled in its pretreatment position, and the chromosomes were rearranged, i.e., the first mitosis was not blocked. During the second mitotic cycle, only a monopolar spindle was assembled in each blastomere and the chromosomes doubled, but cell cleavage was blocked. In the third cycle, mitosis proceeded normally with a bipolar spindle in each blastomere. Flow cytometric analysis of ploidy demonstrated that mitogynogenetic larvae were all diploid. The ultraviolet light-irradiated sperm of the red sea bream (Pagrus major) was condensed, formed a dense chromatin body, and randomly entered one blastomere.

Keywords

CytologyHydrostatic pressureJapanese flounder Paralichthys olivaceusMitogynogenesisMonopolar spindle
Type
Research Article
Information
Zygote , Volume 24 , Issue 5 , October 2016 , pp. 700 - 706
Copyright
Copyright © Cambridge University Press 2016 

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