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Comparison of vitrification and conventional freezing for cryopreservation of caprine embryos

Published online by Cambridge University Press:  25 June 2014

Paula F.B. Araújo-Lemos ,
Leopoldo M. Freitas Neto ,
Marcelo T. Moura ,
Janaína V. Melo ,
Paulo F. Lima  and
Marcos A.L. Oliveira
Paula F.B. Araújo-Lemos
Affiliation:
Empresa Estadual de Pesquisa Agropecuária da Paraíba (EMEPA), Rua Euripedes Tavares, 210, Tambiá, CEP 58013–200, João Pessoa-PB, Brasil.
Leopoldo M. Freitas Neto
Affiliation:
Laboratório de Biotécnicas Aplicadas a Reprodução Animal, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco (UFRPE), Rua Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171–900, Recife-PE, Brasil.
Marcelo T. Moura
Affiliation:
Laboratório de Biotécnicas Aplicadas a Reprodução Animal, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco (UFRPE), Rua Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171–900, Recife-PE, Brasil.
Janaína V. Melo
Affiliation:
Centro de Tecnologias Estratégicas do Nordeste (CETENE), Rua Prof. Luiz Freire nº 01, Cidade Universitária, CEP 50740–540, Recife-PE, Brasil.
Paulo F. Lima
Affiliation:
Laboratório de Biotécnicas Aplicadas a Reprodução Animal, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco (UFRPE), Rua Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171–900, Recife-PE, Brasil.
Marcos A.L. Oliveira*
Affiliation:
Laboratório de Biotécnicas Aplicadas a Reprodução Animal, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco (UFRPE), Rua Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171–900, Recife-PE, Brasil.
*
All correspondence to: Marcos A.L. de Oliveira. Laboratório de Biotécnicas Aplicadas a Reprodução Animal, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco (UFRPE), Rua Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171–900, Recife-PE, Brasil. e-mail: [email protected]
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Summary

The experiment aimed to compare conventional freezing and different vitrification protocols for cryopreservation of caprine embryos at morphological, ultrastructural, and functional levels. Caprine embryos produced in vivo were allocated randomly to three groups: (1) conventional freezing with ethylene glycol (EG); (2) dimethyl sulfoxide + EG (DMSO/EG) vitrification; and (3) dimethylformamide + EG (DMF/EG) vitrification. All groups were scored for cell viability (propidium iodide staining and ultrastructural levels) and re-expansion rate after thawing or warming. Embryos subjected to DMSO/EG vitrification showed higher cell viability (73.33%), compared with DMF/EG vitrification and conventional freezing group embryos (40.00 and 66.66%, respectively). The ultrastructural study revealed that vitrified embryos had greater preservation of cellular structure than embryos from conventional freezing with EG. DMSO/EG vitrification resulted in higher rates of re-expansion in vitro (47.36%) than DMF/EG vitrification (31.58%), and conventional freezing (25.00%). In conclusion, caprine embryos produced in vivo are better cryopreserved after vitrification than conventional freezing, therefore we conclude that DMSO/EG vitrification is the most effective protocol for cryopreservation.

Keywords

BlastocystCryobiologyCryoprotectantGoatSlow freezing
Type
Research Article
Information
Zygote , Volume 23 , Issue 4 , August 2015 , pp. 594 - 602
Copyright
Copyright © Cambridge University Press 2014 

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