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Blastocoele formation and cell allocation to the inner cell mass and trophectoderm in haploid and diploid pig parthenotes developing in vitro

Published online by Cambridge University Press:  26 September 2008

Nam-Hyung Kim ,
Sang Jun Uhm ,
Jin Young Ju ,
Hoon Taek Lee  and
Kil Saeng Chung
Nam-Hyung Kim
Affiliation:
Animal Resoures Research Center, Departmant of Animal Sciences, Kon-Kuk University, Seoul, Korea.
Sang Jun Uhm
Affiliation:
Animal Resoures Research Center, Departmant of Animal Sciences, Kon-Kuk University, Seoul, Korea.
Jin Young Ju
Affiliation:
Animal Resoures Research Center, Departmant of Animal Sciences, Kon-Kuk University, Seoul, Korea.
Hoon Taek Lee
Affiliation:
Animal Resoures Research Center, Departmant of Animal Sciences, Kon-Kuk University, Seoul, Korea.
Kil Saeng Chung*
Affiliation:
Animal Resoures Research Center, Departmant of Animal Sciences, Kon-Kuk University, Seoul, Korea.
*
Kil Saeng Chung, Animal Resources Research Center, Department of Animal Sciences, Kon-Kuk University, Kwangjin-gu, Mojin-dong, Seoul 143-701, Korea.
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Summary

The objective of this study was to determine developmental pattern and cell allocation to the inner cell mass and trophectoderm in haploid and diploid embryos following parthenogenetic activation. In vitro matured porcine oocytes were activated by ethanol treatment and cultured in the presence or absence of cytochalasin B for 5h. The oocytes were then cultured in the NCSU23 for 9 days. The combined treatment with cytochalasin B following ethanol treatment did not increase (p >0.1) the incidence of activation. The incidence of development to the blastocyst stage was higher (p <0.05) in the combined treatments of ethanol and cytochalasin B as compared with ethanol treatment alone. The percentage of oocytes with two female pronuclei was higher (p < 0.01) in oocytes treated with cytochalasin B than that in ethanol treatment alone. Treatment with both ethanol and cytochalasin B increased (p <0.01) the incidence of diploid chromosome spread over just the ethanol treatment alone. The average numbers of total cells and inner cell mass were significantly reduced (p <0.05) in the ethanol treatment alone as compared with the combined cytochalasin B and ethanol treatment. These results suggested that the ploidy may affect blastocoele formation and cell allocation to inner cell mass and trophectoderm in the pig.

Keywords

Inner cell massOocytesParthenogenesisPigPloidyTrophectoderm
Type
Article
Information
Zygote , Volume 5 , Issue 4 , November 1997 , pp. 365 - 370
Copyright
Copyright © Cambridge University Press 1997

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References

Cha, S.K., Kim, N.-H., Lee, S.M., Paik, C.S., Lee, H.T. & Chung, K.S. (1997). Effect of cytochalasin B and cycloheximide on the activation rate, chromosome constituent and in vitro development of porcine oocytes following parthenogenetic stimulation. Reprod. Fertil. Dev., in pressCrossRefGoogle ScholarPubMed
Collas, P. & Robl, J.M. (1990). Factors affecting the efficiency of nuclear transplantation in the rabbit embryo. Biol. Reprod. 43, 877–8CrossRefGoogle ScholarPubMed
Edwards, R.G. (1958). The number of cells and cleavages in haploid, diploid, polyploid, and other heteroploid mouse embryos at 3.5 days gestation. J. Exp. Zool: 137, 349–62.CrossRefGoogle Scholar
Fukui, Y, Sawai, K., Furudate, M, Sato, N., Iwazumi, Y & Ohsaki, K. (1992). Parthenogenetic development of bovine oocytes treated with ethanol and cytochalasin B after in vitro maturation. Mol. Reprod. Dev. 33, 357–62.CrossRefGoogle ScholarPubMed
Funahashi, H., Cantley, T.C., Stumpf, T.T., Terlouw, S.L. & Day, B.N. (1994). In vitro development of in vitro matured porcine oocytes following chemical activation or in vitro fertilization. Biol. Reprod. 50, 1072–7CrossRefGoogle ScholarPubMed
Giles, J.R. & Foote, R.H. (1995). Rabbit blastocyst: allocation of cells to the inner cell mass and trophectoderm. Mol. Reprod. Dev. 41, 204–11.CrossRefGoogle Scholar
Hagen, D.R., Prather, R.S. & First, N.L. (1991). Response of porcine oocytes to electrical and chemical activation during maturation in vitro. Mol. Reprod. Dev. 28, 70–3.CrossRefGoogle ScholarPubMed
Handyside, A.H. & Hunter, S. (1984). A rapid procedure for visualising the inner cell mass and trophectoderm nuclei of mouse blastocysts in situ using polynucleotide-specific fluorochromes. J. Exp. Zool. 231, 429–34.CrossRefGoogle ScholarPubMed
Henery, C.C. & Kaufman, M.H. (1992). Cleavage rate of haploid and diploid parthenogenetic mouse embryos during the preimplantation period. Mol. Reprod. Dev.. 31, 258–63.CrossRefGoogle ScholarPubMed
Kaufman, M.H. (1978). Chromosome analysis of early post implantation presumptive haploid parthenogenetic mouse embryos. J. Embryol. Exp. Morphol. 45, 8–91.Google Scholar
Kaufman, M.H. (1983). The experimental induction of parthenogenesis in the mouse. In Early Development of Mammals, ed. Balls, M. & Wild, A.E. pp. 25–44. Cambridge: Cambridge University Press.Google Scholar
Kaufman, M.H. & Sachs, L. (1975). Complete preimplantation development of haploid and aneuploid parthenogenetic mouse embryos. J. Embryol. Exp. Morphol. 34, 645–55.Google Scholar
Kim, N.-H., Moon, S.J., Prather, R.S. & Day, B.N. (1996 a). Cytoskeletal alteration in aged oocytes and parthenogenesis. Mol. Reprod. Dev.. 43, 248–55.3.0.CO;2-#>CrossRefGoogle ScholarPubMed
Kim, N.-H., Simerly, C., Funahashi, H., Schatten, C. & Day, B.N. (1996 b). Microtubule organization in porcine oocytes during fertilization and parthenogenesis. Biol. Reprod. 54, 1397–l404.CrossRefGoogle ScholarPubMed
Machaty, Z., Mayes, M.A. & Prather, R.S. (1995). Parthenogenetic activation of porcine oocytes with guanosine-5-O (3'-thiotriphosphate). Biol. Reprod. 52, 753–8.CrossRefGoogle ScholarPubMed
Machaty, Z., Funahashi, H., Mayes, M.A., Day, B.N. & Prather, R.S. (1996). Effects of injecting calcium chloride into in vitro matured porcine oocytes. Biol. Reprod. 54, 316–22.CrossRefGoogle ScholarPubMed
Mayes, M.A., Stogsdill, P.L. & Prather, R.S. (1995). Parthenogenetic activation of porcine oocytes by protein kinase inhibition. Biol. Reprod. 53, 270–5.CrossRefGoogle Scholar
Nussbaum, D.J. & Prather, R.S. (1995). Differential effects of protein synthesis inhibitors on porcine oocyte activation. Mol. Reprod. Dev. 41, 70–5.CrossRefGoogle ScholarPubMed
Papaioannou, V.E. & Ebert, K.M. (1988). The preimplantation pig embryo: cell number and allocation to trophectoderm and inner cell mass of the blastocyst in vivo and in vitro. Development 102, 793–803.CrossRefGoogle ScholarPubMed
Petters, R.M. & Wells, K.D. (1993). Culture of pig embryos. J. Reprod. Fertil. Suppl. 48, 61–73.Google ScholarPubMed
Presicce, G.A. & Yang, X. (1994). Parthenogenetic development of bovine oocvtes matured in vitro for 24hr and activated by ethanol and cycloheximide. Mol. Reprod. Dev. 38, 380–5.CrossRefGoogle Scholar
Smith, L.C. & Wilmut, I. (1989). Influence of nuclear and cytoplasmic activity on the development in vivo of sheep embryos after nuclear transplantation. Biol. Reprod. 40, 1027–36.CrossRefGoogle ScholarPubMed
Tarkowski, A.K. (1966). An air-drying method for chromosome preparation from mouse eggs. Cytogenetics 5, 394–400.CrossRefGoogle Scholar
Van Soom, A., Boerjan, M., Ysebaert, M.-T. & De Kruif, A. (1996). Cell allocation to the inner cell mass and the trophectoderm in bovine embryos cultured in two different media. Mol. Reprod. Dev. 45, 171–82.3.0.CO;2-4>CrossRefGoogle Scholar