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Attempt at cloning high-quality goldfish breed ‘Ranchu’ by fin-cultured cell nuclear transplantation

Published online by Cambridge University Press:  25 November 2010

Daisuke Tanaka ,
Akito Takahashi ,
Akinori Takai ,
Hiromi Ohta  and
Koichi Ueno
Daisuke Tanaka*
Affiliation:
Department of Fisheries Science, Kinki University, Nara, Nara 6318505, Japan. Department of Fisheries Science, Kinki University, Nara, Japan.
Akito Takahashi
Affiliation:
Department of Fisheries Science, Kinki University, Nara, Japan.
Akinori Takai
Affiliation:
Human and Environmental Sciences, Osaka Shin-Ai College, Osaka, Osaka 5380053, Japan.
Hiromi Ohta
Affiliation:
Department of Fisheries Science, Kinki University, Nara, Japan.
Koichi Ueno
Affiliation:
Department of Fisheries Science, Kinki University, Nara, Japan.
*
All correspondence to: Daisuke Tanaka. Department of Fisheries Science, Kinki University, Nara, Nara 6318505, Japan. Tel: +81 742 43 6362. Fax: +81 742 43 1316. e-mail: [email protected]
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Summary

The viability of ornamental fish culture relies on the maintenance of high-quality breeds. To improve the profitability of culture operations we attempted to produce cloned fish from the somatic nucleus of the high-quality Japanese goldfish (Carassius auratus auratus) breed ‘Ranchu’. We transplanted the nucleus of a cultured fin-cell from an adult Ranchu into the non-enucleated egg of the original goldfish breed ‘Wakin’. Of the 2323 eggs we treated, 802 underwent cleavage, 321 reached the blastula stage, and 51 reached the gastrula stage. Two of the gastrulas developed until the hatching stage. A considerable number of nuclear transplants retained only the donor nucleus. Some of these had only a 2n nucleus derived from the same donor fish. Our results provide insights into the process of somatic cell nuclear transplantation in teleosts, and the cloning of Ranchu.

Keywords

Carassius auratus auratuscloninggoldfishnuclear transplantation
Type
Research Article
Information
Zygote , Volume 20 , Issue 1 , February 2012 , pp. 79 - 85
Copyright
Copyright © Cambridge University Press 2010

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