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ATP content and maturational/developmental ability of bovine oocytes with various cytoplasmic morphologies

Published online by Cambridge University Press:  01 November 2006

Masashi Nagano ,
Seiji Katagiri  and
Yoshiyuki Takahashi
Masashi Nagano
Affiliation:
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
Seiji Katagiri
Affiliation:
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
Yoshiyuki Takahashi*
Affiliation:
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
*
All correspondence to: Y. Takahashi, Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku Kita 18 Nishi 9, Sapporo 060-0818, Japan. Tel: +81 11 706 5231. Fax: +81 11 706 5232. e-mail: [email protected]
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Summary

We examined the relationship among morphological appearance (six groups) of bovine oocytes, ATP content and maturational/developmental ability. Oocytes with a brown ooplasm (with or without a dark region) had intermediate levels of ATP at the germinal vesicle (GV) stage and showed higher rates of first polar body (PB) extrusion than the other groups. Oocytes with a low level of ATP (oocytes with a pale ooplasm without dark clusters) and oocytes with a high level of ATP (oocytes with a black ooplasm) showed lower rates of PB extrusion. During in vitro maturation, ATP levels in oocytes decreased at around GV breakdown and increased toward metaphase II (MII). MII oocytes having a brown ooplasm with a dark region, which had good developmental capacity, had a relatively high level of ATP. MII oocytes with a brown or pale ooplasm without dark clusters, which had poor developmental capacity, had low ATP levels. MII oocytes with a black ooplasm, which had poor developmental capacity, had an unusually high level of ATP. These results suggest that the morphological appearance of bovine oocytes is closely related to their ATP levels and that cytoplasmic morphology will give an advantage for the selection of oocytes with a high maturational and developmental ability.

Keywords

ATPDevelopmental abilityMaturationMitochondriaOocyte morphology
Type
Research Article
Information
Zygote , Volume 14 , Issue 4 , November 2006 , pp. 299 - 304
Copyright
Copyright © Cambridge University Press 2006

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