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Genetic engineering of bacteria and viruses

Published online by Cambridge University Press:  18 September 2007

Éva Nagy
Éva Nagy
Affiliation:
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
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Abstract

Genetic engineering holds out great promise for the development of improved poultry vaccines. Engineered microbes containing foreign genes from several pathogens provide the basis for multivalent vaccines. The vaccine vector should be non-pathogenic and capable of incorporating and expressing foreigngenes. Subunit proteins expressed by non-avian vectors, such as baculoviruses, can be used to evaluate the nature of their antigenic and protective properties and could find use as subunit or virus-like particle based vaccines. Live recombinant viruses can also be used as vaccines. While RNA viruses such as picornaviruses have been used as vectors, DNA viruses are more easily engineered. Avian poxviruses expressing the Newcastle disease virus (NDV) haemagglutin neuraminidase (HN) and fusion (F) proteins protect against Newcastle disease. Because of their ease of administration through the feed or water, their ability to establish mucosal immunity, and recent advances in genome manipulation, avian adenoviruses are now being developed as recombinant avian vaccines. A recombinant fowl adenovirus expresses a green fluorescent protein reporter gene cloned into a non essential TR-2 region of viral DNA and is also immunogenic. Current efforts are directed at incorporating genes, such as HN from NDV, from avian pathogens into this vector. Genetic engineering can also improve diagnostics. For example, recombinant HN can be used in enzyme-linked immunosorbent assay (ELISA) based NDV diagnostic kits. Baculovirus expressed NDV nucleoprotein (NP) used in a differential ELISA can differentiate between birds immunised with a fowl pox virus expressing NP and NDV infected birds. Polymerase chain reaction (PCR) also has an impact on avian health. PCR can be used in diagnostics and, because of its extreme specificity, in epidemiological studies. Rapid and accurate identification and emerging or re-emerging microbial diseases are some challenges that can be more readily addressed by the application of molecular approaches including genetic engineering. Similarly, the development of multivalent and multi-pathogen vaccines through genetic engineering is certainly a realistic objective. Such vaccines would provide multiple protection in a cost effective manner.

Keywords

Differential diagnosisgenetic engineeringrecombinant virusesvaccine development
Type
Research Article
Information
World's Poultry Science Journal , Volume 57 , Issue 4 , December 2001 , pp. 391 - 400
Copyright
Copyright © Cambridge University Press 2001

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References

Adzhar, A., Shaw, K., Britton, P. and Cavanagh, D. (1996) Universal oligonucleotides for the detection of infectious bronchitis virus by the polymerase chain reaction. Avian Pathology 25: 817–836CrossRefGoogle ScholarPubMed
Alexander, H.S. and Nagy, É. (1997) PCR to detect infectious laryngotracheitis virus in conjunctival swabs from experimentally infected chickens. Avian Diseases 41: 646–653CrossRefGoogle ScholarPubMed
Burgess, S.C. and Davison, T.F. (1999) A quantitative duplex PCR technique for measuring amounts of cell-associated Marek's disease virus: differences in two populations of lymphoma cells. Journal of Virological Methods 82: 27–37CrossRefGoogle ScholarPubMed
Cao, J., Krell, P.J. and Nagy, É. (1998) Sequence and transcriptional analysis of terminal regions of the fowl adenovirus type-8 genome. Journal of General Virology 79: 2507–2516CrossRefGoogle ScholarPubMed
Cavanagh, D., Mawditt, K., Shaw, K., Britton, P. and Witter, R.L. (1997) Towards the routine application of nucleic acid technology for avian disease diagnosis. Acta Veterinaria Hungarica 45: 281–298Google ScholarPubMed
Conzelmann, K-K. (1998) Nonsegmented Negative-strand RNA Viruses: Genetics and Manipulation of Viral Genomes. Annual Review of Genetics 32: 123–162CrossRefGoogle ScholarPubMed
Dimarchi, R., Brooke, G., Gale, C., Cracknell, V., Doel, T. and Mowat, N. (1986) Protection of cattle against foot-and-mouth disease by a synthetic peptide. Science 232: 639–641CrossRefGoogle ScholarPubMed
Fan, H.H., Kleven, S.H. and Jackwood, M.W. (1995) Application of polymerase chain reaction with arbitrary primers to strain identification of Mycoplasrna gallisepticurn. Avian Diseases 39: 729–735CrossRefGoogle Scholar
Herczeg, J., Wehmann, E., Bragg, R.R., Travassos Dias, P.M., Hadjiev, G., Werner, O. and Lomniczi, B. (1999) Two novel genetic groups (VIIb and VIII) responsible for recent Newcastle disease outbreaks in Southern Africa, one (VIIb) of which reached Southern Europe. Archives of Virology 144: 2087–2099CrossRefGoogle ScholarPubMed
Hitt, M.M. and Graham, F. (2000) Adenovirus vectors for human gene therapy. Advances in Virus Research 55: 479–505CrossRefGoogle ScholarPubMed
Jackwood, D.J., and Jackwood, RJ. (1994) Infectious bursal disease viruses: molecular differentiation of antigenic subtypes among serotype 1 viruses. Avian Diseases 38: 531–537CrossRefGoogle ScholarPubMed
Jarvis, D.L. (1997) Baculovirus expression vectors. In: The Baculoviruses (Miller, L.K., Ed.), Plenum Press, New York, pp. 389–431CrossRefGoogle Scholar
Jiang, P., Ojkic, D., Tuboly, T., Huber, P. and Nagy, É. (1999) Application of the polymerase chain reaction to detect fowl adenovirus. Canadian Journal of Veterinary Research 63: 124–129Google Scholar
Kibenge, F.S.B., Qian, B., Nagy, É., Cleghorn, J.R. and Wadowska, D. (1999) Formation of virus-like particles when the polyprotein gene (segment A) of infectious bursal disease virus is expressed in insect cells. Canadian Joiirnal of Veterinary Research 63: 49–55Google ScholarPubMed
Langeveld, J.P.M., Casal, J.I., Osterhaus, A.D., Cortes, E., De Swart, R., Vela, C., Dalsgaard, K., Puijk, W.C., Schaaper, W.M. and Meloen, R.H. (1994) First peptide vaccine providing protection against viral infection in the target animal: Studies of canine parvovirus in dogs. Journal of Virology 68: 4506–4513CrossRefGoogle ScholarPubMed
Lee, L.H. and Lee, K.H. (1997) Application of the polymerase chain reaction for the diagnosis of fowl poxvirus infection. Journal of Virological Methods 63: 113–119CrossRefGoogle Scholar
Lomniczi, B., Wehmann, E., Herczeg, J., Ballagi-Pordany, A., Kaleta, K.E., Werner, O., Meulemans, G., Jorgensen, P.H., Mante, A.P., Gielkens, A.L.J., Capua, I. and Damoser, J. (1998) Newcastle disease outbreaks in recent years in Western Europe were caused by an old (VI) and a novel genotype (VII). Archives of Virology 143: 49–64CrossRefGoogle Scholar
Makkay, A.M., Krell, P.J. and Nagy, E. (1999) Antibody detection based ELISA for NDV-infected or vaccinated chickens versus NDV HN-subunit vaccinated chickens. Veterinary Mirrobiology 66: 209–222CrossRefGoogle ScholarPubMed
McDougal, L.R. and Reid, W.M. (1997) Coccidiosis. In: Diseases of Poultry, 10th Edition (Calnek, B.W., Ed.), Iowa State University Press, Ames, Iowa, USA, pp. 865–883Google Scholar
Moss, B. (1991) Vaccinia virus: a tool for research and vaccine development. Science 252: 1662–1667CrossRefGoogle ScholarPubMed
Nagy, É., Derbyshire, J.B., Dobos, P. and Krell, P.J. (1990) Cloning and expression of NDV hemagglutinin-neuraminidase cDNA in a baculovirus expression vector system. Virology 176: 426–438CrossRefGoogle Scholar
Nagy, É., Krell, P.J., Dulac, G.C. and Derbyshire, J.B. (1991a) Vaccination against Newcastle disease with a recombinant baculovirus hemagglutinin-neuraminidase subunit vaccine. Avian Diseases 35: 585–590CrossRefGoogle ScholarPubMed
Nagy, É., Huber, P., Krell, P.J. and Derbyshire, J.B. (1991b) Synthesis of Newcastle disease virus (NDV)-like envelopes in insect cells infected with a recombinant baculovirus expressing the haemagglutinin-neucaminidase of NDV. Journal of General Virology 72: 753–756CrossRefGoogle ScholarPubMed
Ojkic, D. and Nagy, É. (2000) The complete nucleotide sequence of fowl adenovirus type 8. Journal of General Virology 81: 1833–1837Google ScholarPubMed
Ojkic, D. and Nagy, É. (2001) The long repeat region is dispensable for fowl adenovirus replication in vitru. Virology 283: 197–206CrossRefGoogle Scholar
Paoletti, E. (1996) Application of pox virus vectors to vaccination: an update. Proceeding of the National Academy of Science, 93: 11349–11353CrossRefGoogle ScholarPubMed
Pastoret, P.P. and Brochier, B. (1999) Epidemiology and control of fox rabies in Europe. Vaccine 17: 1750–1754CrossRefGoogle ScholarPubMed
Raue, R. and Hess, M. (1998) Hexon based PCRs combined with restriction enzyme analysis for rapid detection and differentiation of fowl adenoviruses and egg drop syndrome virus. Journal of Virological Methods 73: 211–217CrossRefGoogle ScholarPubMed
Reddy, P.S., Idamakanti, N., Hyun, B.H., Tikoo, S.K. and Babiuk, L. (1999) Development of porcine adenovirus-3 as an expression vector. Journal of General Virology 80: 563–570CrossRefGoogle ScholarPubMed
Reddy, S.K., Sharma, J.M., Ahmad, J., Reddy, D.N., McMillen, J.K., Cook, S.M., Wild, M.A. and Schwartz, R.D. (1996) Protective efficacy of a recombinant herpesvirus of turkeys as an in ovo vaccine against Newcastle and Marek's diseases in specific-pathogen-free chickens. Vaccine 14: 469–477CrossRefGoogle Scholar
Schnitzlein, W.M., Winans, R., Ellsworth, S. and Tripathy, D.N. (1995) Generation of thymidine kinase-deficient mutants of infectious laryngotracheitis virus. Virology 209: 304–314CrossRefGoogle ScholarPubMed
Smith, L.M., Brown, S.R., Howes, K., McLeod, S., Arshad, S.S., Barron, G.S., Venugopal, K., McKay, J.C. and Payne, L.N. (1998) Development and application of polymerase chain reaction (PCR) tests for the detection of subgroup J avian leukosis virus. Virus Research 54: 87–98CrossRefGoogle ScholarPubMed
Stone, G.G., Oberst, R.D., Hays, M.P., McVey, S. and Chengappa, M.M. (1995) Combined PCR-oligonucleotide ligation assay for detection of Salmonella serovars. Journal of Clinical Microbiology 33: 2888–2893CrossRefGoogle ScholarPubMed
Tuboly, T. and Nagy, É. (2001) Construction and characterization of recombinant porcine adenovirus serotype 5 expressing the transmissible gastroenteritis virus spike gene. Journal of General Virology 82: 183–190CrossRefGoogle ScholarPubMed
Wehmann, E., Herczeg, J., Tanyi, J., Nagy, É. and Lomniczi, B. (1999) Lentogenic field isolates of Newcastle disease virus isolated in Canada and Hungary are identical with the vaccine type used in the region. Avian Pathology 28: 6–12CrossRefGoogle ScholarPubMed