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Enzymes in broiler diets with special reference to protease

Published online by Cambridge University Press:  28 June 2013

V. DOSKOVIĆ ,
S. BOGOSAVLJEVIĆ-BOSKOVIĆ ,
Z. PAVLOVSKI ,
B. MILOŠEVIĆ ,
Z. ŠKRBIĆ ,
S. RAKONJAC  and
V. PETRIČEVIĆ
V. DOSKOVIĆ
Affiliation:
University of Kragujevac, Faculty of Agronomy, Cacak, Serbia
S. BOGOSAVLJEVIĆ-BOSKOVIĆ
Affiliation:
University of Kragujevac, Faculty of Agronomy, Cacak, Serbia
Z. PAVLOVSKI
Affiliation:
Insitute for Animal Husbandry, Belgrade-Zemun, Serbia
B. MILOŠEVIĆ
Affiliation:
University of Pristina, Faculty of Agriculture Kosovska Mitrovica, Serbia
Z. ŠKRBIĆ
Affiliation:
Insitute for Animal Husbandry, Belgrade-Zemun, Serbia
S. RAKONJAC*
Affiliation:
University of Kragujevac, Faculty of Agronomy, Cacak, Serbia
V. PETRIČEVIĆ
Affiliation:
Insitute for Animal Husbandry, Belgrade-Zemun, Serbia
*
Corresponding author: [email protected]
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Abstract

Enzyme supplementation of poultry diets is nutritionally, economically and environmentally justified. Enzymes are used to increase the energy value of feed ingredients and enhance the utilisation of protein, fats, carbohydrates and phytin phosphorus from plant materials, leading to a lower excretion rate of undigested nutrients into the environment and, hence, reduced environmental pollution. This is especially important regarding proteases, as the correct digestion of nitrogenous compounds in feed materials is essential for reducing N excretion – a major pollutant worldwide. Numerous studies have shown no adverse effects of enzyme supplementation in broiler diets on body weight, mortality, health, feed intake, FCR, nutrient digestibility, meat quality and production costs. However, there is still a large amount of uncertainty regarding the use of enzymes.

Keywords

broilerdietenzymesprotease
Type
Review Article
Information
World's Poultry Science Journal , Volume 69 , Issue 2 , June 2013 , pp. 343 - 360
Copyright
Copyright © World's Poultry Science Association 2013

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References

AĆAMOVIC, T. (2001) Commercial applications of enzyme technology for poultry production. World's Poultry Science Journal 57: 225-243.CrossRefGoogle Scholar
ADAMS, C.A. (1989) Kemzyme and animal feed digestion. Krmiva 31: 139-144.Google Scholar
ADEMARK, P., LARSSON, M., TJERNELD, F. and STALBRAND, H. (2001) Multiple α-galactosidases from Aspergillus niger: purification, characterization and substrate specifications. Enzyme and Microbial Technology 29: 441-448.CrossRefGoogle Scholar
ALAM, M.J., HOWLIDER, M.A.R., PRAMANIK, M.A.H. and HAQUE, M.A. (2003) Effect of exogenous enzyme in diet on broiler performance. International Journal of Poultry Science 2(2): 168-173.Google Scholar
ALETOR, V.A., HAMID, I.I., NIEB, E. and PFEFFER, E. (2000) Low-protein amino acid-supplemented diets in broiler chickens:effects on performance, carcass characteristics, whole body composition and efficiencies of nutrient utilization. Journal of the Science of Food and Agriculture 80: 547-554.3.0.CO;2-C>CrossRefGoogle Scholar
ANGEL, C.R., SAYLOR, W., VIEIRA, S.L. and WARD, N. (2011) Effects of a monocomponent protease on performance and protein utilization in 7- to 22-day-old broiler chickens. Poultry Science 90: 2281-2286.CrossRefGoogle ScholarPubMed
ANNISON, G. (1997) The use of exogenous enzymes in ruminant diets, in: CORBET, J.L., CHOCT, M., NOLAN, J.V. & ROWE, J.B. (Eds) Recent Advances in Animal Nutrition in Australia, pp. 8-16 (University of New England, Armidale).Google Scholar
BAAS, T.C. and THACKER, P.A. (1996) Impact of gastric pH on dietary enzyme activity and survivability in swine fed β-glucanase supplemented diets. Canadian Journal of Animal Science 36: 245-252.CrossRefGoogle Scholar
BARBOSA, N.A.A., NILVA, K.S., JOÃO BATISTA, K.F. and LEILANE, R.B.D. (2008) Enzimas exógenas no desempenho e na digestibilidade ileal de nutrientes em frangos de corte. Pesquisa Agropecuária Brasileira 43(6): 755-762.CrossRefGoogle Scholar
BEDFORD, M.R. (2000) Exogenous enzymes in monogastric nutrition-Their current value and future benefits. Animal Feed Science and Technology 86: 1-13.CrossRefGoogle Scholar
BEDFORD, M.R. (2002) The foundation of conducting feed enzyme research and the challenge of explaining the results. Journal of Applied Poultry Research 11(4): 464-470.CrossRefGoogle Scholar
BEDFORD, R. and APAJALAHTI, J. (2001) Microbial interactions in response to exogenous enzyme utilization, in: BEDFORD, M.R. & PARTRIGE, G.G. (Eds) Enzymes in Farm Animal Nutrition pp. 299-314 (CABI Publish, Wallingford, Oxfordshire).Google Scholar
BERTECHINI, A.G., CARVALHO, J.C.C., MESQUITA, F.R., CASTRO, S.F., MENEGHETTI, C. and SORBARA, J.O.B. (2009) Use of a protease to enhance the utilization of soybean meal amino acids by broilers. Poultry Science Association 98th Annual Meeting July 20-23, 2009 Raleigh, North Carolina. Poultry Science 88 (Supplement 1): Abstract 221, 68.Google Scholar
BOGOSAVLJEVIĆ-BOŠKOVIĆ, S., RAKONJAC, S., DOSKOVIĆ, V. and PETROVIĆ, D.M. (2012) Broiler rearing systems: a review of major fattening results and meat quality traits. World's Poultry Science Journal 68(2): 217-228.CrossRefGoogle Scholar
BRAKE, J., WILLIAMS, C.V. and LENFESTEY, B.A. (2003) Optimization of dietary phosphorus for broiler breeders and their progeny. Proceedings of Alltech's 19th Annual Symposium, Lexington, KY: 77-83 (Nottingham Univ. Press, Nottingham, UK).Google Scholar
BREGENDAHL, K., SELL, J.L. and ZIMMERMAN, D.R. (2002) Effect of low-protein diets on growth performance and body composition of broiler chicks. Poultry Science 81:1156-1167.CrossRefGoogle ScholarPubMed
BROZ, J. and WARD, N.E. (2007) The role of vitamins and feed enzymes in combating metabolic challenges and disorders. Journal of Applied Poultry Research 16: 150-159.CrossRefGoogle Scholar
BRUFAU, J., FRANCESCH, M. and PÉREZ-VENDRELL, A.M. (2002) Exogenous enzymes in poultry feeding. Recent developments. In 2002 Annual Animal Nutrition Conference; Fayetteville: Arkansas, USA.Google Scholar
BURNETT, G.S. (1966) The effect of damaged starch, amylolytic enzymes, and proteolytic enzymes on the utilization of cereals by chickens. British Poultry Science 3: 89-103.CrossRefGoogle Scholar
CAFÉ, M.B., BORGES, C.A., FRITTS, C.A. and WALDROUP, P.W. (2002) Avizyme Improves Performance of Broilers Fed Corn-Soybean Meal-Based Diets. Journal of Applied Poultry Research 11: 29-33.CrossRefGoogle Scholar
CAINE, W.R., VERSTEGEN, M.W.A., SAUER, W.C., TAMMINGA, S. and SCHULZE, H. (1998) Effect of protease treatment of soybean meal on content of total soluble matter and crude protein level of soybean trypsin inhibitors. Animal Feed Science and Technology 71: 177-183.CrossRefGoogle Scholar
CARVALHO, J.C.C., BERTECHINI, A.G., MESQUITA, F.R., RIOS, R.L., LIMA E.M.C., and SORBARA, J.O.B. (2009a) Use of a protease to enhance the utilization of meat and bone meal amino acids by broilers. Poultry Science Association 98th Annual Meeting July 20-23, 2009 Raleigh, North Carolina. Poultry Science Volume 88 (Supplement 1): Abstract 219, pp.37.Google Scholar
CARVALHO, J.C.C., BERTECHINI, A.G., RIOS, R.L., MESQUITA, F.R., LIMA, E.M.C. and SORBARA, J.O.B. (2009b) Use of a protease to enhance the utilization of corn amino acids by broilers. Poultry Science Association 98th Annual Meeting July 20-23, 2009 Raleigh, North Carolina. Poultry Science Volume 88 (Supplement 1), Abstract 220, pp. 68.Google Scholar
CHOCT, M. (2006) Enzymes for the feed industry: past, present and future. World's Poultry Science Journal 62: 5-15.CrossRefGoogle Scholar
CMILJANIĆ, R., PAVLOVSKI, Z., TRENKOVSKI, S., LUKIĆ, M., ŠKRBIĆ, Z. and MARINKOV, G. (2007) Uticaj primene enzima u ishrani živine na proizvodne rezultate i kvalitet proizvoda. Biotechnology in Animal Husbandry 23(1-2): 49-57.Google Scholar
COWIESON, A.J. (2005) Factors that affect the nutritional value of maize for broilers. Animal Feed Science and Technology 119: 293-305.CrossRefGoogle Scholar
COWIESON, A.J. and RAVINDRAN, V. (2008) Effect of exogenous enzymes in maize-based diets varying in nutrient density for young broilers: growth performance and digestibility of energy, minerals and amino acids. British Poultry Science 49: 37-44.CrossRefGoogle ScholarPubMed
COWIESON, J. and ADEOLA, O. (2005) Carbohydrases, Protease, and Phytase Have an Additive Beneficial Effect in Nutritionally Marginal Diets for Broiler Chicks. Poultry Science 84:1860-1867.CrossRefGoogle ScholarPubMed
DIERICK, N.A. and DECUYPERE, J.A. (1994) Enzymes and growth in pigs., in: COLE, D.J.A., WISEMAN, J. & VARLEY, M. (Eds) Principles of Pig Science, pp. 169-195 (Nottingham University Press, Nottingham).Google Scholar
DOUGLAS, M.W., PARSONS, C.M. and BEDFORD, M.R. (2000) Effect of various soybean meal sources and Avizyme on chick growth performance and ileal digestible energy. Journal of Applied Poultry Research 9(1): 74-80.CrossRefGoogle Scholar
DOURADO, L.R.B., SAKOMURA, N.K., BARBOSA, N.A.A., BONATO, M.A., KAWUAUCHI, I.M., FERNANDES, J.B.K. and COSTA, F.G.P. (2009) Corn and Soybean Meal Metabolizable Energy with the Addition of Exogenous Enzymes for Poultry. Revista Brasileira de Ciência Avícola 11(1): 51-55.CrossRefGoogle Scholar
EBERT, A.R., KESSLER, A.M., PENZ, J.R., POPHAL, S. and RIBEIRO, A.M.L. (2000) Effect of adding Vegpro in two energy level diets on the performance of broilers exposed to heat stress. Poultry Science 79(S1): 19-20 (abstr.).Google Scholar
ENGBERG, R.M., HEDEMANN, M.S. and JENSEN, B.B. (2002) The influence of grinding and pelleting of feed on the microbial composition and activity in the digestive tract of broiler chickens. British Poultry Science 43: 569-579.CrossRefGoogle ScholarPubMed
ESPINO, T.M., LUIS, E.S., SAPIN, A.B., TAMBALO, R.D. and UNIDA, F.B. (2000) Acid protease from Monascus sp. BIOTECH 3064 and other microbial enzymes and feed additives in broiler diets. Proceedings of the 29. Annual Convention of the Philippine Society for Microbiology, Ilocos Norte (Philippines), pp. 121-133.Google Scholar
FAVERO, A., MAIORKA, A., ROCHA, C., APPELT, M.D. and SORBARA, J.O.B. (2009) Effect of protease enzyme on performance and ileal digestibility of broilers grown to 42 days of age in floor pens. International Poultry Scientific Forum, Atlanta, Georgia, Abstracts of Papers M28, pp. 9.Google Scholar
FIDELIS, F., KLUENTER, A., FISCHER, M. and PONTOPPIDAN, K. (2010) A Feed Serine Protease Improves Broiler Performance and Increases Protein and Energy Digestibility. The Journal of Poultry Science 48(4): 239-246.Google Scholar
FISCHER, G., MAIER, J.C., RUTZ, F. and BERMUDEZ, V.L. (2002) Desempenho de frangos de corte alimentados com dietas à base de milho e farelo de soja, como u sem adição de enzimas. Revista Brasileira de Zootecnia 31(1): 402-410.CrossRefGoogle Scholar
FISCHER, M., GLITSOE, V., PETTERSSON, D. and FRU, F. (2009) Efficacy of a novel feed protease on a variety of protein ingredients. International Poultry Scientific Forum, Atlanta, Georgia, Abstracts of Papers, T191, pp. 60.Google Scholar
FRIETAS, D.M., VIEIRA, S.L., ANGEL, C.R., FAVERO, A. and MAIORKA, A. (2011) Performance and nutrient utilization of broilers fed diets supplemented with a novel mono-component protease. Journal of Applied Poultry Research 20: 322-334.CrossRefGoogle Scholar
GARCÍA, E.R.M, MURAKAMI, A.E., BRANCO, A.F., FURLAN, A.C. and MOREIRA, I. (2000) Efeito da suplementação enzimática em rações com farelo de soja e soja integral extrusada sobre a digestibilidade de nutrientes, o fluxo de nutrientes na digesta ileal e o desempenho de frangos. Revista Brasileira de Zootecnia 29(5): 1414-1426.CrossRefGoogle Scholar
GARCIA, A.R., BATAL, A.B. and DALE, N.M. (2006) Biological Availability of Phosphorus Sources in Prestarter and Starter Diets for Broiler Chicks. Journal of Applied Poultry Research 15(4): 518-524.CrossRefGoogle Scholar
GHAZI, S., ROOKE, J.A. and GALBRAITH, H. (2003) Improvement of the nutritive value of soybean meal by protease and alpha-galactosidase treatment in broiler cockerels and broiler chicks. British Poultry Science 44: 410-418.CrossRefGoogle ScholarPubMed
GHAZI, S., ROOKE, J.A., GALBRAITH, H. and BEDFORD, M.R. (1997) Effect of adding protease and alpha-galactosidase enzyme to soybean meal on nitrogen retention and true metabolisable energy in broilers. British Poultry Science 38(supplement): S28.Google Scholar
GHAZI, S., ROOKE, J.A., GALBRAITH, H. and BEDFORD, M.R. (2002) The potential for the improvement of the nutritive value of soya-beanmeal by different proteases in broiler chicks and broiler cockerels. British Poultry Science 43: 70-77.CrossRefGoogle ScholarPubMed
GRACIA, M.I., ARANIBAR, M.J., LAZARO, R., MEDEL, P. and MATEOS, G.G. (2003) Alpha-amylase supplementation of broiler diets based on corn. Poultry Science 82: 436-442.CrossRefGoogle ScholarPubMed
GÜNAL, M., YASAR, S. and FORBES, J.M. (2004) Performance and some digesta parameters of broiler chickens given low or high viscosity wheat-based diets with or without enzyme supplementation. Turkish Journal of Veterinary and Animal Sciences 28: 323-327.Google Scholar
HARTMAN, R. (1996) Wheat-based diets improved by enzymes. Journal of Applied Poultry Research 5:167-172.Google Scholar
HÜBNER, K., VAHJEN, W. and SIMON, O. (2002) Bacterial responses to different dietary cereal type and xylanase supplementation in the intestine of broiler chicken. Archives of Animal Nutrition 56: 167-187.Google Scholar
HUO, G., YANG, L. and LI, S. (2001) Tolerance of broiler to dietary soybean antinutritional factors. Journal of Northeast Agricultural University 8: 290-299.Google Scholar
HUO, G.C., FOWLER, V.R., INBORR, J. and BEDFORD, M.R. (1993) The use of enzymes to denature antinutritive factors in soybean. Proceedings of the Second International Workshop on Antinutritional Factors in Legume Seed, Wageningen, Netherlands, pp. 60.Google Scholar
IJI, P.A., KHUMALO, K., SLIPPERS, S. and GOUS, R.M. (2003) Intestinal function and body growth of broiler chickens on diets based on maize dried at different temperatures and supplemented with a microbial enzyme. Reproduction Nutrition Development 43: 77-90.CrossRefGoogle ScholarPubMed
JENSEN, L.S., FRY, R.E., ALRED, J.B. and MCGINNIS, J. (1957) Improvement in the nutritional value of barley for chicks by enzyme supplementation. Poultry Science 36: 919-921.CrossRefGoogle Scholar
JÓZEFIAK, D., RUTKOWSKI, A., FRTCZAK, M. and BOROS, D. (2004) The effect of dietary fibre fractions from different cereals and microbial enzymes supplementation on performance, ileal viscosity and short-chain fatty acids concentration in caeca of broiler chickens. Journal of Animal and Feed Sciences 13(3): 487-496.CrossRefGoogle Scholar
KOCHER, A., CHOCT, M., MORRISROE, L. and BROZ, J. (2001) Effects of enzyme supplementation on the replacement value of canola meal for soybean meal in broiler diets. Australian Journal of Agricultural Research 52: 447-452.CrossRefGoogle Scholar
LESLIE, M.A., MORAN, E.T. and BEDFORD, M.R. (2005) The effects of phytase and glycanase supplementation to corn soy diets on AME. Poultry Science 84 (Suppl. 1): 106 (Abstr.).Google Scholar
LONGO, F.A., MENTEN, J.F.M., PEDROSO, A.A., FIGUEIREDO, A.N., RACANICCI, A.M.C. and SORBARA, J.O.B. (2007) Performance and Carcass Composition of Broilers Fed Different Carbohydrate and Protein Sources in the Prestarter Phase. Journal of Applied Poultry Research 16(2): 171-177.CrossRefGoogle Scholar
MADRID, J., CATALÁ-GREGORI, P., GARCÍA, V. and HERNÁNDEZ, F. (2010) Effect of a multi-enzyme complex in wheat-soybean meal diet on digestibility of broiler chickens under different rearing conditions. Italian Journal of Animal Science 2010 volume 9: e1 doi:10.4081/ijas.2010.e1.CrossRefGoogle Scholar
MAHAGNA, M., NIR, I., LARBIER, M. and NITSAN, Z. (1995) Effect of age and exogenous amylase and protease on development of the digestive tract, pancreatic enzyme activities and digestibility of nutrients in young meat-type chicks. Reproduction Nutrition Development 35: 201-212.CrossRefGoogle ScholarPubMed
MAIORKA, A., FAVERO, A., MEURER, R.F.P., MORAES, M.T.T. and SORBARA, J.O.B. (2009) Broiler chicken performance and ileal digestibility is improved by a protease used in corn/soybean meal/meat bone meal diet. International Poultry Scientific Forum, Atlanta, Georgia, Abstracts of Papers, T102, pp. 33.Google Scholar
MARSMAN, G.J., GRUPPEN, H., VAN DER POEL, A.F., KWAKKEL, R.P., VERSTEGEN, M.W. and VORAGEN, A.G. (1997) The effect of thermal processing and enzyme treatment of soybean meal on growth performance, illeal nutrient digestibility and chyme characteristics in broiler chicks. Poultry Science 76: 864-872.CrossRefGoogle Scholar
MORGAN, A.J. and BEDFORD, M.R. (1995) Wheat-specific feed enzymes. Feed Compounder 15: 25-27.Google Scholar
MULYANTINI, N.G.A., CHOCT, M., LI, X. and LOLE, U.R. (2005) The effect of xylanase, phytase and lipase supplementation on the performance of broiler chickens fed a diet with a high level of rice bran. Proceedings of the Australian Poultry Science Symposium 17: 305-307.Google Scholar
NAVEED, A., AĆAMOVIC, T. and BEDFORD, M.R. (1999) The influence of carbohydrase and protease supplementation on aminoacid digestibility of lupin-based diets for broiler chicks. Proceedings of the Australian Poultry Science Symposium 11: 93-96.Google Scholar
O’-DOHERTY, J.V. and FORDE, S. (1999) The effect of protease and alpha-galactosidase supplementation on the nutritive value of peas for growing and finishing pigs. Irish Journal of Agricultural & Food Research 38: 217-226.Google Scholar
ODETALLAH, N.H., WANG, J.J., GARLICH, J.D. and SHIH, J.C.H. (2005) Versazyme Supplementation of Broiler Diets Improves Market Growth Performance. Poultry Science 84: 858-864.CrossRefGoogle ScholarPubMed
OFFICER, D.I. (1995) Effect of multi-enzyme supplements on the growth performance of piglets during the pre- and post-weaning periods. Animal Feed Science and Technology 56: 55-65CrossRefGoogle Scholar
OFFICER, D.I. (2000) Feed Enzymes, in: MELLO, J.P.F. (Ed.) Farm Animal Metabolism and Nutrition, pp. 405-426 (CAB International).CrossRefGoogle Scholar
OLUKOSI, O.A., COWIESON, A.J. and ADEOLA, O. (2007) Age-related influence of a cocktail of xylanase, amylase, and protease or phytase individually or in combination in broilers. Poultry Science 86(1): 77-86CrossRefGoogle Scholar
ONDERCI, M., SAHIN, N., SAHIN, K., CIKIM, G., AYDIN, A., OZERCAN, I. and AYDIN, S. (2006) Efficacy of supplementation of α-amylase-producing bacterial culture on the performance, nutrient use, and gut morphology of broiler chickens fed a corn-based diet. Poultry Science 85: 505-510.CrossRefGoogle ScholarPubMed
OSEI, S.A. and ODURO S., (2000) Effects of dietary enzyme on broiler chickens fed diets containing wheat bran. Journal of Animal and Feed Sciences 9: 681-686.CrossRefGoogle Scholar
OUHIDA, I., PEREZ, J.F., ANGUITA, M. and GASA, J. (2002) Influence of β-Mannase on Broiler Performance, Digestibility, and Intestinal Fermentation. Journal of Applied Poultry Research 11: 244-249.CrossRefGoogle Scholar
PACK, M. and BEDFORD, M. (1997) Feed enzymes for corn and soybean broiler diets. World's Poultry Science Journal 13: 87-93.Google Scholar
PALACIOS, M.F., EASTER, R.A., SOLTWEDEL, K.T., PARSONS, C.M., DOUGLAS, M.W., HYMOWITZ, T. and PETTIGREW, J.E. (2004) Effect of soybean variety and processing on growth performance of young chicks and pigs. Journal of Animal Science 82: 1108-1114.CrossRefGoogle ScholarPubMed
PARTRIDGE, G.G. (1993) In feed enzymes and antibodies. Pig Veterinary Journal 31: 34-50.Google Scholar
PERIĆ, L., MILOŠEVIĆ, N., ĐUKIĆ-STOJČIĆ, M., BJEDOV, S. and RODIĆ, V. (2008) Effect of Enzymes on Performances of Broiler Chickens. Biotechnology in Animal Husbandry 24(5-6): 45-51.CrossRefGoogle Scholar
RAHMAN, A., AHMED, S., KHAN, D., HUSSAIN, M., AHMAD, I., SHAH, Z., MOHAMMAD SOHAIL, S., IKRAM-UL-HAQ, , HAFEEZ, A. and ZIA-UR-REHMAN, (2009) Nutritional Importance Of Exogenous Enzymes In Broiler Ration At Finisher Phase. Sarhad Journal of Agriculture 25(3): 475-478.Google Scholar
RANADE, A.S. and RAJMANE, B.V. (1992) Effect of enzyme feed supplement on commercial broilers. Proceedings of the 19th World's Poultry Congress. Amsterdam, The Netherlands, Vol. 2: 485-487.Google Scholar
RAVINDRAN, V., SELLE, P.H. and BRYDEN, W.L. (1999) Effects of phytase supplementation, individually and in combination, with glycanase, on the nutritive value of wheat and barley. Poultry Science 78: 1588-1595.CrossRefGoogle ScholarPubMed
RICHTER, G., CYRIACI, G., SCHWARTZE, J., FLACHOWSKY, G. and HENNING, A. (eds.) (1991) Effectiveness of enzymes in broiler diets. Vitamins und weiters zusatzstoffe bei mensch und Tier. 3. Symposium, Friedrich-Schiller-Universitat, Jena, Germany: 384-387.Google Scholar
RODRIGUES, P.B. (2000) Digestibilidade de nutrients e valores energeticos de racoes e alguns ahmentos para frangos de corte. Ph.D. Thesis, Universidade Federal de Viçosa.Google Scholar
ROSA, A.P., SCHER, A., STEFANELLO, C., DIAZ, E., DUARTE, V., OICHENAZ, N. and SORBARA, J.O.B. (2009) Effect of pure protease enzyme and dietary protein/amino acids levels on broiler performance. International Poultry Scientific Forum, Atlanta, Georgia, Abstracts of Papers, T101, pp. 33.Google Scholar
ROSTANGO, H.S., TEJEDOR, A.A., ALBINO, L.F. and SILVA, J.H. (2000) Enzyme supplementation of corn-soybean meal diets improves nutrients ileal digestibility in broiler chicks Proceedings Biotechnology In Feed Industries Alltech's16th Annual Symposium Nottingham Univ. Press, UK.:101-107.Google Scholar
RUBIO, L.A., BRENES, A. and CENTENO, C. (2003) Effects of feeding growing broiler chickens with practical diets containing sweet lupin (Lupinus angustifolius) seed meal. British Poultry Science 44: 391-397.CrossRefGoogle ScholarPubMed
SALEH, F., OHTSUKA, A., TANAKA, T. and HAYASHI, K. (2004) Carbohydrases are digested by proteases present in enzyme preparations during in vitro digestion. The Journal of Poultry Science 41(3): 229-235.CrossRefGoogle Scholar
SAYYAZADEH, H., RAHIMI, G. and REZAEI, M. (2006) Influence of enzyme supplementation of maize, wheat and barley-based diets on the performance of broiler chickens. Pakistan Journal of Biological Sciences 9(4): 616-621.CrossRefGoogle Scholar
SIMBAYA, J., SLOMINSKI, B.A., GUENTER, W., MORGAN, A. and CAMPBELL, L.D. (1996) The effects of protease and carbohydrase supplementation on the nutritive value of canola meal for poultry: in vitro and in vivo studies. Animal Feed Science and Technology 61: 219-234.CrossRefGoogle Scholar
SUN, X. (2007) Effect of Corn Quality and Enzyme Supplementation on Broiler Performance, Gastrointestinal Enzyme Activity, Nutrient Retention, Intestinal Mucin, and Jejunal Gene Expression. Ph. D. Thesis, Faculty of the Virginia Polytechnic Institute and State University Blacksburg, Virginia.Google Scholar
SZCZUREK, W., KORELESKI, J., HANCZAKOWSKI, P. and SZYMCZYK, B. (2001) Improving nutritive value of a chicken diet with a high concentration of thermally damaged meat meal by enzyme supplementation. Annals of Animal Science 1: 139-151.Google Scholar
THORPE, J. and BEAL, J.D. (2001) Vegetable Protein Meals and the Effects of Enzymes, in: BEDFORD, M.R. & PARTRIDGE, G.G. (Eds) Enzymes in Farm Animal Nutrition, pp. 125-143 (CABI Publishing, Wallingford, Oxford, UK).Google Scholar
TUOYING, A.O. (2005) Exogenous enzymes and organic acids in the nutrition of broiler chicks: effects on growth performance and in vitro and in vivo digestion. Ph. D. Thesis, The Graduate School University of Kentucky.Google Scholar
UNI, Z., NOY, Y. and SKLAN, D. (1999) Posthatch development of small intestinal function in the poult. Poultry Science 78: 215-222.CrossRefGoogle ScholarPubMed
VIEIRA, S.L., FREITAS, D.M., PEÑA, J.E.M., BARROS, R., XAVIER, P.S., VIAN, A.C. and SORBARA, J.O.B. (2009) Performance and amino acid utilization by broilers supplemented with a novel exogenous protease. International Poultry Scientific Forum, Atlanta, Georgia, Abstracts of Papers, T114, pp. 37.Google Scholar
WU, Y.B., RAVINDRAN, V., HENDRIKS, W.H., MOREL, P.C.H. and PIERCE, J. (2004a) Evaluation of a microbial phytase, produced by solid state fermentation, in broiler diets II: Influence on phytate hydrolysis, apparent metabolizable energy, and nutrient utilization. Journal of Applied Poultry Research 13: 561-569.CrossRefGoogle Scholar
WU, Y.B., RAVINDRAN, V., THOMAS, D.G., BIRTLES, M.J. and HENDRIKS, W.H. (2004b) Influence of phytase and xylanase, individually or in combination, on performance, apparent metabolisable energy, digestive tract measurements and gut morphology in broiler fedwheat-based diets containing adequate level of phosphorus. British Poultry Science 45: 76-84.CrossRefGoogle ScholarPubMed
YADAV, J.L. and SAH, R.A. (2005) Supplementation Of Corn-Soybean Based Broiler's Diets With Different Levels Of Acid Protease. Journal of the Institute of Agriculture and Animal Science 26: 65-70.CrossRefGoogle Scholar
YADAV, J.L. and SAH, R.A. (2006) Supplementation Of Corn-Soybean Based Layers Diets With Different Levels Of Acid Protease. Journal of the Institute of Agriculture and Animal Science 27: 93-102.CrossRefGoogle Scholar
YU, B., WU, S.T., LIU, C.C., GAUTHIER, R. and CHIOU, P.W.S. (2007) Effects of enzyme inclusion in a maize-soybean diet on broiler performance . Animal Feed Science and Technology 134: 283-294.CrossRefGoogle Scholar
ZAKARIA, H.A.H., JALAL, M.A.R. and ABU ISHMAIS, M.A. (2010) The Influence of Supplemental Multi-enzyme Feed Additive on the Performance, Carcass Characteristics and Meat Quality Traits of Broiler Chickens. International Journal of Poultry Science 9(2): 126-133.CrossRefGoogle Scholar
ZANELLA, I., SAKOMURA, N.K., SILVERSIDES, F.G., FIQUEIRDO, A. and PACK, M. (1999) Effect of enzyme supplementation of broiler diets based on maize and soybeans. Poultry Science 78(4): 561-568.CrossRefGoogle Scholar
ZHAO, H. and WANG, K.N. (2008) Effects of different enzyme complex supplements in corn-soybean feedstuff on growth performance and nutrient utilization of broilers. Journal of Chongqing University (English Edition) 7(2): 158-164.Google Scholar
ZHOU, Y., JIANG, Z., LV, D. and WANG, T. (2009) Improved energy-utilizing efficiency by enzyme preparation supplement in broiler diets with different metabolizable energy levels. Poultry Science 88: 316-322.CrossRefGoogle ScholarPubMed
ZYLA, K., LEDOUX, D.R., KUJAWSKI, M. and VEUM, T.L. (1996) The efficacy of an enzymic cocktail and a fungal mycelium in dephosphorylating corn-soybean meal-based feeds fed to growing turkeys. Poultry Science 75: 381-387.CrossRefGoogle Scholar