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New insights in biologically active proteins and peptides derived from hen egg

Published online by Cambridge University Press:  18 September 2007

Y. Mine*
Affiliation:
Department of Food Science, University of GuelphGuelph, Ontario N1G 2W1, Canada
J. Kovacs-Nolan
Affiliation:
Department of Food Science, University of GuelphGuelph, Ontario N1G 2W1, Canada
*
*Corresponding author: [email protected]*
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Abstract

Bioactive peptides are specific protein fragments that positively impact the body's function or condition and ultimately may influence health. These peptides are inactive within the sequence of the parent protein and can be released during proteolysis or fermentation. They may exert a number of different activities in vivo, affecting cardiovascular, endocrine, immune and nervous system in addition to nutrient utilization. Hen eggs have traditionally been recognized as an excellent source of protein, vitamins and minerals. Research in the past decade, however, has produced a substantial amount of evidence indicating that hen egg proteins and peptides may exert several diverse biological effects, above and beyond fulfilling basic nutritional requirements. Several biological activities have now been associated with hen egg proteins, including novel antimicrobial activities, immunomodulatory, anti-cancer, and anti-hypertensive activities, highlighting the importance of hen egg proteins in human health, and disease prevention and treatment. Continued research to identify new and existing biological functions of hen egg proteins and their derivatives will help to define new methods to further improve the value of eggs, as a source of numerous biologically active compounds with specific benefits for human and animal health, and secure their role in the therapy and prevention of chronic and infectious disease.

Type
Reviews
Copyright
Copyright © Cambridge University Press 2006

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Footnotes

From a paper presented at the XIth European Symposium on the Quality of Eggs and Egg Products, Doorwerth, The Netherlands, 23–26 May 2005

References

Abdallah, F.B. and Chahine, J.M. (1999) Transferrins, the mechanism of iron release by ovotransferrin. European Journal of Biochemistry 263: 912920.CrossRefGoogle ScholarPubMed
Aguilera, O., Quiros, L.M. and Fierro, J.F. (2003) Transferrins selectively cause ion efflux through bacterial and artificial membranes. FEBS Letter 548: 510.CrossRefGoogle ScholarPubMed
Asakura, K., Kojima, T., Shirasaki, H. and Kataura, A. (1990) Evaluation of the effects ofantigen specific immunotherapy on chronic sinusitis in children with allergy. Auris Nasus Larynx 17: 3338.Google Scholar
Banks, J.G., Board, R.G. and Sparks, N.H.C. (1986) Natural antimicrobial systems and their potentialin food preservation of the future. Biotechnology and Applied Biochemistry 8: 103147.Google Scholar
Baron, F., Fauvel, S. and Gautier, M. (2000) Behaviour of Salmonella enteritidis in industrial eggwhite: egg naturally contains factors inhibitory to salmonella growth. In: Egg Nutrition and Biotechnology; Sim, J.S.; Nakai, N.; Guenter, W., Eds; CAB International: Oxon, UK pp. 417430.Google Scholar
Brady, D., Gaines, S., Fenelon, L., McPartlin, J. and O'farrelly, C.A. (2002) A lipoproteinderivedantimicrobial factor from hen-egg yolk is active against Streptococcus species. Journal of Food science 67: 30963103.CrossRefGoogle Scholar
Burley, R.W. and Vadehra, D.V. (1989) The Avian Egg, Chemistry and Biology. John Wiley & Sons Inc.: New York, New York.Google Scholar
Burley, R.W. and Cook, W.H. (1961) Isolation and composition of avian egg yolk granules and theirconstituents α- and β-lipovitellines. Canadian Journal of Biochemical Physiology 39: 12951307.CrossRefGoogle Scholar
Brady, D., Gaines, S., Fenelon, L., McPartlin, J. and O'farrelly, C.A. (2002) A lipoproteinderivedantimicrobial factor from hen-egg yolk is active against Streptococcus species. Journal of Food science 67: 30963103.CrossRefGoogle Scholar
Carlander, D., Kollberg, H., Wejaker, P.E. and Larsson, A. (2000) Peroral immunotherapy with yolk antibodies for the prevention and treatment of enteric infections. Immunology Research 21: 16.CrossRefGoogle ScholarPubMed
Choi, I., Jung, C., Seog, H. and Choi, H. (2004) Purification of phosvitin from egg yolk anddetermination of its physiochemical properties. Food Science and Biotechnology 13: 434437.Google Scholar
Cotterill, O.J. and Geiger, G.S. (1977) Egg product yield trends from shell eggs. Poultry Science 56: 10271031.CrossRefGoogle Scholar
Das, S., Banerjee, S. and Gupta, J.D. (1992) Experimental evaluation of preventative and therapeuticpotential of lysozyme. Chemotherapy 38: 350357.CrossRefGoogle Scholar
Davalos, A., Miguel, M., Bartolome, B. and Lopez-Findino, R. (2004) Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis. Journal of Food Protection 67: 19391944.Google Scholar
Fan, X., Subramaniam, R., Weissm, M.F. and Monnier, V.M. (2003) Methylglyoxal-bovine serumalbumin stimulates tumor necrosis factor alpha secretion in RAW 264.7 cells through activation of mitogenactivatingprotein kinase, nuclear factor kappaB and intracellular reactive oxygen species formation. Archivesof Biochemistry and Biophysics 409: 274286.CrossRefGoogle Scholar
Fujita, H., Sasaki, R. and Yoshikawa, M. (1995a) Potentiation of the antihypertensive activity oforally administered ovokinin, a vasorelaxing peptide derived from ovalbumin, by emulsification in eggphosphatidylcholine. Bioscience Biotechnology and Biochemistry 59: 23442345.CrossRefGoogle Scholar
Fujita, H., Usui, H., Kurahashi, K. and Yoshikawa, M. (1995b) Isolation and characterization ofovokinin, a bradykinin B1 agonist peptide derived from ovalbumin. Peptides 16: 785790.CrossRefGoogle ScholarPubMed
Giansanti, F., Rossi, P.Massucci, M.T., Botti, D., Antonini, G., Valenti, P. and Seganti, L. (2002) Antiviral activity of ovotransferrin discloses an evolutionary strategy for the defensive activities oflactoferrin. Biochemistry and cell biology 80: 125130.CrossRefGoogle Scholar
Goldberg, J., Shrikant, P. and Mescher, M.F. (2003) In vivo augmentation of tumor-specific CTLresponses by class I/peptide antigen complexes on microspheres (large multivalent immunogen). Journal ofImmunology 170: 228235.Google Scholar
Hatta, H., Tsuda, K., Ozeki, M., Kim, M.Yamamoto, T., Otake, S., Horosawa, M.Katz, J., Childers, N.K. and Michalek, S.M. (1997) Passive immunization against dental plaque formationin humans: Effect of a mouth rinse containing egg yolk antibodies (IgY) specific to Streptococcus mutans. Caries Research 31: 268274.CrossRefGoogle Scholar
He, X., Tsang, T.C., Luo, P., Zhang, T. and Harris, D.T. (2003) Enhanced tumor immunogenicitythrough coupling cytokine expression with antigen presentation. Cancer Gene Therapy 10: 669677.CrossRefGoogle ScholarPubMed
Hirota, Y., Yang, M.P., Araki, S., Yoshihara, K., Furusawa, S., Yasuda, M., Mohamed, A., Matsumoto, Y. and Onodera, T. (1995) Enhancing effects of chicken egg white derivatives onthe phagocytic response in the dog. Journal of Veterinary Medical Science 57: 825829.Google Scholar
Holen, E., Bolann, B. and Elsayed, S. (2001) Novel B and T cell epitopes of chicken ovomucoid (Gald 1) induce T cell secretion of IL-6, IL-13, and IFN-gamma. Clinical Experimental Allergy 31: 952964.CrossRefGoogle Scholar
Horie, K., Horie, N., Abdou, A.M., Yang, J-O, Yun, S.S., Chun, C.K., Kim, M. and Hatta, H. (2004) Suppressive effect of functional drinking yogurt containing specific egg yolk immunoglobulin onHelicobacter pylori in humans. Journal of Dairy Science 87: 4703–4079.Google Scholar
Ibrahim, H.R., Sugimoto, Y. and Aoki, T. (2000) Ovotransferrin antimicrobial peptide (OTAP-92) killsbacteria through a membrane damage mechanism. Biochimica et Biophysica Acta 1523: 196205.Google Scholar
Ibrahim, H.R., Thomas, U. and Pellegrini, A. (2001) A helix-loop peptide at the upper lip of theactive site cleft of lysozyme confers potent antimicrobial activity with membrane permeabilization action. Journal of Biological Chemistry 276: 4376743774.CrossRefGoogle Scholar
Ishikawa, S., Yano, Y., Arihara, K. and Itoh, M. (2004) Egg yolk phosvitin inhibits hydroxyl radicalformation from the Fenton reaction. Bioscience Biotechnology and Biochemistry 68: 13241331.CrossRefGoogle Scholar
Jiang, B. and Mine, Y. (2000) Preparation of novel functional oligophosphopeptides from hen egg yolkphosvitin. Journal of Agricultural Food Chemistry 48: 990994.CrossRefGoogle Scholar
Jiang, B. and Mine, Y. (2001) Phosphopeptides derived from hen egg yolk phosvitin: effect of molecular sizeon the calcium-binding properties. Bioscience Biotechnology and Biochemistry 65: 11871190.Google Scholar
Kassaify, Z.G. and Mine, Y. (2004a) Non-immunized egg yolk powder can suppress the colonization of Salmonella typhimurium, E coli 0157:H7 and Campylobacter jejuni in laying hens. Poultry Science 83: 14971506.CrossRefGoogle Scholar
Kassaify, Z.G., Li, E.W.Y. and Mine, Y. (2005) Identification of antiadhesive fractions in nonimmunizedegg yolk powder: In vitro study. Journal of Agricultural Food Chemistry 53: 46074614.Google Scholar
Kassaify, Z.G. and Mine, Y. (2004b) Effect of food protein supplements on Salmonella enteritidis infectionand prevention in laying hens. Poultry Science 83: 753760.CrossRefGoogle Scholar
Kato, T., Imatani, T., Miura, T., Minaguchi, K., Saitoh, E. and Okuda, K. (2000) Cytokineinducingactivity of family 2 cystatins. Biological Chemistry 381: 11431147.Google Scholar
Kobayashi, K., Hattori, M., Hara-Kudo, Y., Okubo, T., Yamamoto, S., Takita, T. and Sugita-Konishi, Y. (2004) Glycopeptide derived from hen egg ovomucin has the ability to bindenterohemorrhagic Escherichia coli 0157:H7. Journal of Agricultural Food Chemistry 52: 57405746.Google Scholar
Kollberg, H., Carlander, D., Olesen, H., Wejaker, P.E., Johannesson, M. and Larsson, A. (2003) Oral administration of specific yolk antibodies (IgY) may prevent Pseudomonasaeruginosa infections in patients with cystic fibrosis: A phase I feasibility study. Pediatric pulmonology 35: 433440.Google Scholar
Korpela, J., Salonen, E.M., Kuusela, P., Sarvas, M. and Vaheri, A. (1984) Binding of avidinto bacteria and to the outer membrane porin of Escherichia coli. FEMS Microbiological Letter 22: 310.CrossRefGoogle Scholar
Kovacs-Nolan, J. and Mine, Y. (2004) Avian egg antibodies: Basic and potential applications. Avian poultry Biology Review 15: 2546.Google Scholar
Kruger, C., Pearson, S.K., Kodama, Y., Vacca Simith, A., Bowen, W.H. and Hammarstron, L. (2004) The effects of egg-derived antibodies to glucotransferases on dental caries inrats. Caries Research 38: 914.Google Scholar
Lee-Huang, S., Huang, P.L., Sun, Y., Huang, P.L., Kumg, H.F., Bithe, D.L. and Chen, H.C. (1999) Lysozyme and RNases as anti-HIV components in beta-core preparations of human chorionicgonadotropin. Proceedings of the National Academy of Sciences of the United States of America 96: 26782681.Google Scholar
Li-Chan, E. and Nakai, S. (1989) Biochemical basis for the properties of egg white. Critical Review ofPoultry Biology 2: 2158.Google Scholar
Li-Chan, E., Powrie, W.D. and Nakai, S. (1995) The Chemistry of eggs and egg products. In: Eggscience and Technology, 4th Edition; Stadelman, W. J.; Cotterill, O. J., Eds.; The Haworth Press Inc.: Newyork, New York pp. 105175.Google Scholar
Losso, J.N., Nakai, S. and Charter, E.A. (2000) Lysozyme. In: Natural Food Antimicrobial Systems; Naidu, A. S., Ed.; CRC Press Inc.: New York, New York pp. 185210.Google Scholar
Lu, C.L. and Baker, R. (1986) Characteristics of egg yolk phosvitin as an antioxidant for inhibiting metalcatalyzedphospholipid oxidations. Poultry Science 65: 20652070.CrossRefGoogle ScholarPubMed
Matoba, N., Usui, H., Fujita, H. and Yoshikawa, M. (1999) A novel anti-hypertensive peptidederived from ovalbumin induces nitric oxide-mediated vasorelaxation in an isolated SHR mesenteric artery. FEBS Letter 452: 181184.Google Scholar
Matoba, N., Yamada, Y., Usui, H., Nakagiri, R. and Yoshikawa, M. (2001) Designing potentderivatives of ovokinin(2–7), an anti-hypertensive peptide derived from ovalbumin. Bioscience Biotechnologyand Biochemistry 65: 736739.Google Scholar
Miguel, M., Recio, I., Gomez-Ruiz, J.A., Ramos, M. and Lopez-Fandino, R. (2004) Angiotensin I-converting enzyme inhibitory activity of peptides derived from egg white proteins byenzymatic hydrolysis. Journal of Food Protection 67: 19141920.Google Scholar
Mine, Y., Ma, F. and Lauriau, S. (2004) Antimicrobial peptides released by enzymatic hydrolysis of henegg white lysozyme. Journal of Agriculture and Food Chemistry 52: 10881094.Google Scholar
Nagaoka, S., Masaoka, M., Zhang, Q., Hasegawa, M. and Watanabe, K. (2002) Eggovomucin attenuates hypercholesterolemia in rats and inhibits cholesterol absorption in Caco-2 cells. Lipids 37: 267272.Google Scholar
Oguro, T, Ohaki, Y., Asano, G., Ebina, T. and Watanabe, K. (2001) Ultrastructural andimmunohistochemical characterization on the effect of ovomucin in tumor angiogenesis. Japanese Journal of Clinical Electron Microscopy 33: 8999.Google Scholar
Otani, H. and Odashima, M. (1997) Inhibition of proliferative responses of mouse spleen lymphocytes by lacto- and ovotransferrins. Food Agriculture and Immunology 9: 193202.CrossRefGoogle Scholar
Pacor, S., Gagliard, R., Di Daniel, E., Vadori, M. and Sava, G. (1999) In vitro down regulationof ICAM-1 and E-cadherin and in vitro reduction of lung metastases of TS/A adenocarcinoma by a lysozymederivative. International of Journal Molecular Medecine 4: 369375.Google Scholar
Pellergrini, A., Thomas, U., Bramaz, N., Klauser, S., Hunziker, P. and Vonfellenberg, R. (1997) Identification and isolation of a bactericidal domain in chicken egg whitelysozyme. Journal of Applied Microbiology 82: 372378.Google Scholar
Pellergrini, A, Thomas, U., Wild, P., Schraner, E. and Von Fellenberg, R. (2000) Effectof lysozyme or modified lysozyme fragments on DNA and RNA synthesis and membrane permeability of Escherichia coli. Microbiology Research 155: 6977.Google Scholar
Pellergrini, A., Hulsmeier, A., Hunziker, P. and Thomas, U. (2004) Proteolytic fragments ofovalbumin display antimicrobial activity. Biochimica et Biophysica Acta 1672: 7685.CrossRefGoogle Scholar
Sava, G., Ceschia, V., Pacor, S. and Zabucchi, G. (1991) Observations on the antimetastatic actionof lysozyme in mice bearing Lewis lung carcinoma. Anticancer Research 11: 11091113.Google Scholar
Sava, G., Pacor, S., Dasic, G. and Bergamo, A. (1995) Lysozyme stimulates lymphocyte response toConA and IL-2 and potentiates 5-fluorouracil action on advanced carcinomas. Anticancer Research 15 18831888.Google ScholarPubMed
Sava, G. (1996) Pharmacological aspects and therapeutic applications of lysozymes. Experimental Science 75: 433449.Google Scholar
Sharon, N. and Ofek, I. (2002) Fighting infectious diseases with inhibitors of microbial adhesion to hosttissues. Critical Review of Food Science and Nutrition 42: 267272.Google Scholar
Smith, D.J., King, W.F. and Godiska, R. (2001) Passive transfer of immunoglobulin Y to Streptococcusmutans glucan binding protein B can confer protection against experimental dental caries. Infection andImmunity 69: 31353142.Google Scholar
Sugahara, T., Murakami, F., Yamada, Y. and Sasaki, T. (2000) The mode of actions of lysozymeas an immunoglobulin production stimulating factor. Biochimica et Biophysica Acta 1475: 2734.Google Scholar
Sugino, H., Nitoda, T. and Juneja, L.R. (1997) General chemical composition of hen eggs. In: Heneggs, Their Basic and Applied Science; Yamamoto, T.; Juneja, L. R.; Hatta, H.; Kim, M., Eds.; CRC Press Inc.: New York, New York pp. 1324.Google Scholar
Tanizaki, H., Tanaka, H., Iwata, H. and Kato, A. (1997) Activation of macrophages by sulfatedglycopeptides in ovomucin, yolk membrane, and chalazae in chicken eggs. Bioscience, Biotechnology and Biochemistry 61: 18831889.Google Scholar
Tezuka, H. and Yoshikawa, M. (1995) Abstract of Annual Meeting of Japan Society for BioscienceBiotechnology, and Agrochemistry, Tokyo pp 163.Google Scholar
Tsuge, Y., Shimoyamada, M. and Watanabe, K. (1996a) Binding of egg white proteins to viruses. Bioscience, Biotechnology and Biochemistry 60: 15031504.Google Scholar
Tsuge, Y., Shimoyamada, M. and Watanabe, K. (1996b) Differences in hemagglutination inhibitionactivity against bovine rotavirus and hen newcastle disease virus based on the subunits in hen egg whiteovomucin. Bioscience, Biotechnology and Biochemistry 60: 15051506.Google Scholar
Tsuge, Y., Shimoyamada, M. and Watanabe, K. (1997a) Structural features of newcastle diseasevirus- and anti-ovomucin antibody-binding glycopeptides from pronase-treated ovomucin. Journal ofAgriculture and Food Chemistry 45: 23932398.Google Scholar
Tsuge, Y., Shimoyamada, M. and Watanabe, K. (1997b) Bindings of ovomucin to newcastle diseasevirus and anti-ovomucin antibodies and its heat stability based on binding abilities. Journal of Agriculture andFood Chemistry 45: 46294634.Google Scholar
Valenti, P., Antonini, G., Von Hunolstein, C., Visca, P., Orsi, N. and Antonini, E. (1983) Studies of the antimicrobial activity of ovotransferrin. International Journal of Tissue Research 5: 97105.Google Scholar
Verdot, L., Lalmanach, G., Vercruysse, V., Hartmann, S., Lucius, R., Hoebeke, J., Gauthier, F. and Vray, B. (1996) Cystatins up-regulate nitric oxide release from interferon-gammaactivatedmouse peritoneal macrophages. Journal of Biological Chemistry 271: 2807728081.Google Scholar
Verdot, L., Lalmanach, G., Vercruysse, V., Hoebeke, J., Gauthier, F. and Vray, B. (1999) Chicken cystatin stimulates nitric oxide release from interferon-gamma-activated mouse peritonealmacrophages via cytokine synthesis. European Journal of Biochemistry 266: 11111117.Google Scholar
Vidovic, D., Graddis, T., Chen, F., Slagle, P., Diegel, M., Stepan, L. and Laus, R. (2002) Antitumor vaccination with HER-2-derived recombinant antigens. International Journal of Cancer 102: 660664.Google Scholar
Wahn, V. (2003) Primary immunodeficiencies caused by defects of cytokines and cytokine receptors. In: Methods in Molecular Biology: Cytokines and Colony Stimulating Factors: Methods and Protocols; Korholz, D.; Kiess, W., Eds.; Humana Press Inc.: Totowa, New Jersey Vol. 215, pp. 312.Google Scholar
Watanabe, K., Ysuge, Y., Shimoyamada, M., Ogama, N. and Ebina, T. (1998) Antitumor effectsof pronase-treated fragments, glycopeptides, from ovomucin in hen egg white in a double grafted tumorsystem. Journal of Agriculture and Food Chemistry 46: 30333038.Google Scholar
Xie, H., Huff, G.R., Huff, W.E., Balog, J.M. and Rath, N.C. (2002) Effects of ovotransferrin onchicken macrophages and heterophil-granulocytes. Developmental and Comparative Immunology 26: 805815.Google Scholar
Yamada, Y., Matoba, N., Usui, H., Onishi, K. and Yoshikawa, M. (2002) Design of a highlypotent anti-hypertensive peptide based on ovokinin(2–7). Bioscience, Biotechnology and Biochemistry 66: 12131217.Google Scholar
Yoshikawa, M. and Fujita, H. (1994) Studies on the optimum conditions to utilize biologically activepeptides derived from food proteins. In: Developments in Food Engineering; Yano, T; Matsuno, R.; Nakamura, K., Eds.; Blackie Academic and Professional: New York, New York pp. 10531055.Google Scholar