Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-26T11:10:22.922Z Has data issue: false hasContentIssue false

IgY – Immune component of eggs as a source of passive immunity for animals and humans

Published online by Cambridge University Press:  12 July 2010

M.E. COOK*
Affiliation:
Department of Animal Science, University of Wisconsin, Madison, WI 53706, USA
D.L. TROTT
Affiliation:
Department of Nutritional Science, University of Wisconsin, Madison, WI 53706, USA
*
Corresponding author: [email protected]
Get access

Abstract

Antibodies deposited in the avian egg have been found to be a platform for the production of a diverse array of safe commercial products for improving animal health and the efficiency of their production. This review provides over 20 years of experience and comments on the production, storage, stabilisation and use of egg yolk antibodies. Host targeted immuno-therapies presented in this review provides the reader with new areas of research and discovery. Standards for vaccination protocols, methods of isolating and storing the antibodies are also presented. Areas needing to advance the commercial use of egg antibodies, including methods to improve antibody titre, methods to heat stabilise the antibody molecule, and the identification of new uses and targets are also discussed. Examples of successful uses of egg antibodies as feed additives are also presented.

Type
Review Article
Copyright
World's Poultry Science Association 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

AKITA, E.N. and NAKAI, S. (1992) Immunoglobulins from yolk: isolation and purification. Journal of Food Science 57: 629-634.CrossRefGoogle Scholar
BANIK, M.T., PAUL, J.A., BURDSALL, H.H. and COOK, M.E. (1993) Serological differentiation of two forms of Phellinus weirii. Mycologia 85: 605-611.CrossRefGoogle Scholar
BENEDICT, A.A. and BERESTECKY, J.M. (1987) Special features of avian immunoglobulins, in: TOIVANEN, A. and TOIVANEN, P. (Eds) Avian Immunology: Basis and Practice, pp. 113-125 (CRC Press, Boca Raton, FL, USA).Google Scholar
BERGHMAN, L.R., ABI-GHANEM, D., WAGHELA, S.D. and RICKE, S.C. (2005) Antibodies. An alternative for antibiotics? 2005. Poultry Science 84: 660-666.CrossRefGoogle Scholar
BOBECK, E. and COOK, M. (2005) Heat stability of gallus domesticus immunoglobulin Y (IgY). Wisconsin Journal of Science 1: 25-28.Google Scholar
BOBECK, E.A., TROTT, D.L., COOK, M.E. and YANG, M. (2006) Methods in heat-stabilization of gallus domesticus immunoglobulin Y (IgY). Poultry Science 85, supplement 1: 122.Google Scholar
BOBECK, E.A., COOK, C.L., GELBACH, B.E., YANG, M. and COOK, M.E. (2008) Thermoprotection of bioactive proteins added to animal feed. World's Poultry Science Journal 64, supplement 2: 199.Google Scholar
BURDSALL, H.H., BANIK, M. and COOK, M.E. (1990) Serological differentiation of three species of Armillaria and Lentinula edodes by enzyme-linked immunosorbent assay using immunized chickens as a source of antibodies. Mycologia 82: 415-423.CrossRefGoogle Scholar
CARLANDER, D., KOLLBERG, H., WEJAKER, P.E. and LARSSON, A. (2000) Peroral immunotherapy with yolk antibodies for the prevention and treatment of enteric infections. Immunology Research 21: 1-6.CrossRefGoogle ScholarPubMed
CHANG, H.M., LU, T.C., CHEN, C.C., TU, Y.Y. and HWANG, J.Y. (2000) Isolation of immunoglobulin from egg yolk by anionic polysaccharides. Journal of Agriculture Food Chemistry 48: 995-999.CrossRefGoogle ScholarPubMed
CHEN, C.C., TU, Y.Y., CHEN, T.L. and CHANG, H.M. (2002) Isolation and characterization of immunoglobuling in yolk (IgY) specific against hen egg white lysozyme by immunoaffinity chromatography. Journal of Agriculture Food Chemistry 50: 5425-5428.CrossRefGoogle Scholar
COOK, M.E. (1991) Nutrition and the immune response of the domestic fowl. CRC Poultry Biology 3: 167-189.Google Scholar
COOK, C.L., PAO, W., FIRCA, J.R., ANDERSON, B.E. and FRYER, J.P. (2001) Simple purification methods for an alpha galactose-specific antibody from chicken eggs. Journal of Bioscience Bioengineer 91: 305-310.CrossRefGoogle Scholar
COOK, M.E. (2004) Antibodies: alternatives to antibiotics in improving growth and feed efficiency. Journal of Applied Poultry Research 13: 106-119.CrossRefGoogle Scholar
DANG, H.C. and VISEK, W.J. (1960) Effect of urease injection on body weight of growing rats and chicks. Proceedings of Society of Experimental Biological Medicine 105: 164-167.CrossRefGoogle ScholarPubMed
GEE, S.C., BATE, I.M., THOMAS, T.M. and RYLATT, D.B. (2003) The purification of IgY from chicken egg yolk by preparative electrophoresis. Protein Expression Purification 30: 151-155.CrossRefGoogle ScholarPubMed
HATTA, H., TSUDA, K., AKACHI, S., KIM, M. and YAMAMOTO, T. (1993a) Productivity and some properties of egg yolk antibody (IgY) against human rotavirus compared with rabbit IgG. Bioscience Biotechnology Biochemistry 57: 450-454.CrossRefGoogle ScholarPubMed
HATTA, H., MABE, K., KIM, M., YAMAMOTO, T., GUTIERREZ, M.A. and MIYAZAKI, T. (1993b) Oral passive immunization effect of anti-human rotavirus IgY and its behaviour against proteolytic enzymes. Bioscience Biotechnology Biochemistry 57: 1077-1081.CrossRefGoogle ScholarPubMed
HIGGINS, D.A. (1996) Comparative immunology of avian species, in: DAVISON, T.F., MORRIS, T.R. and PAYNE, L.N. (Eds) Poultry Immunology, pp. 149-208 (Carfax Publishing, Abington, Oxfordshire, England).Google Scholar
KOVACS-NOLAN, J. and MINE, Y. (2004a) Avian egg antibodies: basic and potential applications. Avian Poultry Biology Reviews 15: 25-46.CrossRefGoogle Scholar
KOVACS-NOLAN, J. and MINE, Y. (2004b) Passive immunization through avian egg antibodies. Food Biotechnology 18: 39-62.CrossRefGoogle Scholar
KOVACS-NOLAN, J. and MINE, Y. (2005) Microencapsulation for the gastric passage and controlled intestinal release of immunoglobulin Y. Journal of Immunological Methods 296: 199-209.CrossRefGoogle ScholarPubMed
KOVACS-NOLAN, J., PHILLIPS, M. and MINE, Y. (2005) Advances in the value of eggs and egg components for human health. Journal of Agriculture Food Chemistry 53: 8421-8431.CrossRefGoogle ScholarPubMed
KOWALCZYK, K., DAISS, J., HALPEM, J. and ROTH, T.F. (1985) Quantification of maternal-fetal IgG transport in the chicken. Immunology 54: 755-762.Google Scholar
KRAMER, T.T. and CHO, H.G. (1970) Transfer of immunoglobulins and antibodies in the hen's egg. Immunology 19: 157-167.Google ScholarPubMed
LI, X., NAKANO, T., SUNWOOD, H.H., PAEK, B.H., CHAE, H.S. and SIM, J.S. (1998) Effects of egg and yolk weights on yolk antibody (IgY) production in laying chickens. Poultry Science 77: 266-270.CrossRefGoogle ScholarPubMed
LI, X.Y., JIN, L.J., McALLISTER, T.A., STANDFORD, K., XU, J.Y., LU, Y.H., ZHEN, Y.H., SUN, Y.X. and XU, Y.P. (2007) Chitosan-alginate microcapsules for oral delivery of egg yolk immunoglobulin (IgY). Journal of Agriculture Food Chemistry 55: 2911-2917.CrossRefGoogle ScholarPubMed
LILIUS, E.M. and MARNILA, P. (2001) The role of colostral antibodies in prevention of microbial infections. Current Opinions on Infectious Disease 14: 295-300.CrossRefGoogle ScholarPubMed
LIN, S.S. and HIGGINS, D.A. (1990) Yolk-sac transmission of serum immunoglobulins in the duck (Anas platyrhynchos). Comparative Biochemistry Physiology 97: 637-644.Google Scholar
PIMENTEL, J.L. and COOK, M.E. (1988) Improved growth in the progeny of hens immunized with jackbean urease. Poultry Science 67: 434-439.CrossRefGoogle ScholarPubMed
PIMENTEL, J.L., COOK, M.E. and JONSSON, J.M. (1991) Research Note: Increased growth of chicks and poults obtained from hens injected with jackbean urease. Poultry Science 70: 1842-1844.CrossRefGoogle ScholarPubMed
POLSON, A., VON WECHMAR, M.B. and VAN REGENMORTEL, M.H. (1980) Isolation of viral IgY antibodies from yolks of immunized hens. Immunology Communications 9: 475-493.CrossRefGoogle ScholarPubMed
POLSON, A., COETZER, T., KRUGER, J., MALTZAHN, E.V. and VAN DER MERWE, K.J. (1985) Improvements in the isolation of IgY from the yolks of eggs laid by immunized hens. Immunological Investigations 14: 323-327.CrossRefGoogle ScholarPubMed
POLSON, A. (1990) Isolation of IgY from the yolks of eggs by chloroform polyethylene glycol procedure. Immunological Investigations 19: 253.Google ScholarPubMed
REILLY, R.M., DOMING, R. and SANDHU, J. (1997) Oral delivery of antibodies. Future pharmacokinetics trends. Clinical Pharmacokinetics 32: 313-323.CrossRefGoogle ScholarPubMed
RICKE, S.C., SCHAEFER, D.M., COOK, M.E. and KANG, K.H. (1988) Differentiation of ruminal bacterial species by enzyme-linked immunosorbent assay using egg yolk antibodies from immunized chicken hens. Applied Environmental Microbiology 54: 596-599.CrossRefGoogle ScholarPubMed
ROOS, N., MAHE, S., BENAMOUZIG, R., SICK, H., RAUTUREA, J. and TOME, D. (1995) 15N-labeled immunoglobulins from bovine colostrums are partially resistant to digestion in human intestine. Journal of Nutrition 125: 1238-1244.Google ScholarPubMed
ROSE, M.E., ORLANS, E. and BUTTRESS, N. (1974) Immunoglobulin classes in the hen's egg: Their segragation in the yolk and white. European Journal of Immunology 4: 521.CrossRefGoogle Scholar
ROSE, M.E. and ORLANS, E. (1981) Immunoglobulins in the egg, embryo and young chick. Developmental Comparative Immunology 5: 15-20.CrossRefGoogle ScholarPubMed
SAHIM, O., ZHANG, Q., MEITZLER, J.C., HARR, B.S., MORISHITA, T.Y. and MOHAN, R. (2001) Prevalence, antigenic specificity, and bactericidal activity of poultry anti-campylobacter maternal antibodies. Applied Environmental Microbiology 67: 3951-3957.CrossRefGoogle Scholar
SCHADE, R., PFISTER, C., HALATSCH, R. and HENKLEIN, P. (1991) Polyclonal IgY antibodies from chicken egg yolk an alternative to the production of mammalian IgG type antibodies in rabbits. Alternatives to Laboratory Animals 19: 403-419.CrossRefGoogle Scholar
SCHADE, R., BEHN, I., ERHARD, M., HLINAK, A. and STAAK, C. (Eds) (2001) Chicken Egg Yolk Antibodies, Production and Application - IgY Technology. Springer, Berlin, Germany.CrossRefGoogle Scholar
SHAWKY, S.A., SAIF, Y.M. and McCORMICK, J. (1994) Transfer of maternal anti-rotavirus IgG to the mucosal surfaces and bile of turkey poults. Avian Diseases 38: 409-417.CrossRefGoogle Scholar
SHAWKY, S.A., SAIF, Y.M. and SWAYNE, D.E. (1993) Role of circulating maternal anti-rotavirus IgG in protection of intestinal mucosal surface in turkey poults. Avian Diseases 37: 1041-1050.CrossRefGoogle ScholarPubMed
SHIMIZU, M., HAGASHIMA, H., SANO, K., HASHIMOTO, K., OZEKI, M., TSUDA, K. and HATTA, H. (1992) Molecular stability of chicken and rabbit immunoglobulin G. Bioscience, Biotechnology, Biochemistry 56: 270-274.CrossRefGoogle ScholarPubMed
SHIMIZU, M., MIWA, Y., HASHIMOTO, K. and GOTO, A. (1993) Encapsulation of chicken egg yolk immunoglobulin G (IgY) by liposomes. Bioscience, Biotechnology, Biochemistry 57: 1445-1449.CrossRefGoogle ScholarPubMed
SHIMIZU, M., NAGASHIMA, H., HASHIMOTO, K. and SUZUKI, T. (1994) Egg yolk antibody (IgY) stability in aqueous solution with high sugar concentrations. Journal of Food Science 59: 763-766.CrossRefGoogle Scholar
STONE, H.D., BRUGH, M. and XIE, Z. (1992) Simulation of maternal immunity by inoculation of immune yolk preparations into the yolk sac of 1 day-old chickens. Avian Diseases 36: 1048-1051.CrossRefGoogle ScholarPubMed
SUZUKI, N. and LEE, Y.C. (2004) Site-specific N-glycosylation of chicken serum IgG. Glycobiology 14: 275-292.CrossRefGoogle ScholarPubMed
TAYLOR, A.L., FABIANE, S.M., SUTTON, B.J. and CALVERT, R.A. (2009) The crystal structure of an avian IgY-Fc fragment reveals conservation with both mammalian IgG and IgE. Biochemistry 48: 558-562.CrossRefGoogle ScholarPubMed
TINI, M., JEWELL, U.R., CAMENISCH, G., CHILOV, D. and GASSMAN, M. (2002) Generation and application of chicken egg-yolk antibodies. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 131: 569-574.CrossRefGoogle ScholarPubMed
TROTT, D.L., YANG, M. and COOK, M.E. (2005) Effect of laying hen age on antibody deposition in egg yolk. Poultry Science 84, supplement 1: 46.Google Scholar
TROTT, D.L., YANG, M., UTTERBACK, P.L., KOELKEBECK, K.W. and COOK, M.E. (2006) Methods to improve the economics of egg antibody production. Poultry Science 85, supplement 1: 79.Google Scholar
TROTT, D.L., YANG, M., UTTERBACK, P.L., UTTERBACK, C.W., KOELKEBECK, K.W. and COOK, M.E. (2009a) Utility of spent Single Comb White Leghorn hens for production of polyclonal egg yolk antibody. Journal of Applied Poultry Research 18: 679-689.CrossRefGoogle Scholar
TROTT, D.L., YANG, M., GONZALEZ, J., LARSON, A.E., TEPP, W.H., JOHNSON, E.A. and COOK, M.E. (2009b) Egg yolk antibodies for detection and neutralization of Clostridium botulinum type A neurotoxin. Journal of Food Protection 72: 1005-1011.CrossRefGoogle ScholarPubMed
TROTT, D.L., YANG, M., HELLESTAD, E.M. and COOK, M.E. (2008) Additions of killed whole cell bacteria preparations to Freund complete adjuvant alter laying hen antibody response to soluble protein antigen. Poultry Science 87: 912-917.CrossRefGoogle ScholarPubMed
WARNY, M., FATIMI, A., BOSTWICK, E.F., LAINE, D.C., LEBEL, F., LAMONT, J.T., POTHOULAKIS, C. and KELLY, C.P. (1999) Bovine immunoglobulin concentrate-Clostridium difficile retains C difficile toxin neutralizing activity after passage through the human stomach and small intestine. Gut 44: 212-217.CrossRefGoogle ScholarPubMed
WARR, G.W., MAGOR, K.K. and HIGGINS, D.A. (1995) IgY- Clues to the origins of modern antibodies. Immunology Today 16: 392-398.CrossRefGoogle Scholar
YOKOYAMA, H., PERALTA, R.C., SENDO, S., IKEMORI, Y. and KODAMA, Y. (1993) Detection of passage and absorption of chicken egg yolk immunoglobulins in the gastrointestinal tract of pigs by use of enzyme-linked immunosorbent assay and fluorescent testing. American Journal of Veterinary Research 54: 867-872.CrossRefGoogle ScholarPubMed