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The chicken embryo and its micro environment during egg storage and early incubation

Published online by Cambridge University Press:  12 November 2008

I.A.M. REIJRINK ,
R. MEIJERHOF ,
B. KEMP  and
H. VAN DEN BRAND
I.A.M. REIJRINK*
Affiliation:
HatchTech Incubation Technology B.V., PO Box 256, 3900 AG Veenendaal, The Netherlands
R. MEIJERHOF
Affiliation:
HatchTech Incubation Technology B.V., PO Box 256, 3900 AG Veenendaal, The Netherlands
B. KEMP
Affiliation:
Adaptation Physiology Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands
H. VAN DEN BRAND
Affiliation:
Adaptation Physiology Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands
*
Corresponding author: [email protected]
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Abstract

When egg storage periods are prolonged (>7 days), hatchability and chick quality declines. The reason for this decline has been investigated, but is still not completely understood. At oviposition the developmental stage of the chicken embryo varies and so do the total number of viable cells. During storage, changes can occur in the embryo. Embryo viability at the end of storage seems to be dependent on the number of viable cells and the developmental stage of the embryo at oviposition. When the hypoblast is completely formed (during the quiescent developmental stage), the embryo seems to be more able to endure prolonged storage periods than embryos that are less or more advanced. During storage, changes also occur in egg characteristics such as albumen viscosity, albumen pH and yolk pH. There appears to be an interaction between albumen pH and embryo viability during early incubation and perhaps also during storage. An albumen pH of 8.2 seems to be optimal for embryo development. Albumen pH may influence embryo viability, but embryo viability may in turn, affect albumen pH. It has been hypothesised that an embryo in which the hypoblast is completely formed is better able to provide an effective barrier between the internal embryo and the exterior (yolk and albumen) and/or is better able to produce sufficient amount of carbon dioxide, which will reduce the pH level in the micro environment of the embryo to the optimal pH of 8.2. It appears that, to maintain hatchability and chick quality after prolonged storage periods, embryonic development should be advanced to the stage in which the hypoblast is completely formed or the atmosphere during storage and early incubation should be altered in such a way that albumen pH is maintained at the optimal level of 8.2.

Keywords

embryo viabilityalbumen pHegg storagepre-warming durationearly incubation
Type
Review Article
Information
World's Poultry Science Journal , Volume 64 , Issue 4 , December 2008 , pp. 581 - 598
Copyright
Copyright © World's Poultry Science Association 2008

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References

ARORA, K.L. and KOSIN, I.L. (1966) Changes in the gross morphological appearance of chicken and turkey blastoderms during preincubation storage. Poultry Science 45: 819-825.Google Scholar
ARORA, K.L. and KOSIN, I.L. (1968) The response of the early chicken embryo to pre-incubation temperature as evidenced from its gross morphology and mitotic pattern. Physiological Zoology 41: 104-112.CrossRefGoogle Scholar
ASMUNDSON, V.S. and MACILRAITH, J.J. (1948) Preincubation tests with turkey eggs. Poultry Science 27: 394-401.CrossRefGoogle Scholar
BAKST, M.R. and AKUFFO, V. (1999) Impact of egg storage on embryonic development. Avian and Poultry Reviews 13: 125-131.Google Scholar
BAKST, M.R. and HOLM, L. (2003) Impact of egg storage on carbonic anhydrase activity during early embryogenesis in the turkey. Poultry Science 82: 1193-1197.Google Scholar
BAKST, M.R. and HOWARTH, B. Jr. (1977) Hydrolysis of the hen's perivitelline layer by cock sperm. Biology of Reproduction 17: 370-379.Google Scholar
BECKER, W.A. (1964) The storage of white leghorn hatching eggs in plastic bags. Poultry Science 43: 1109-1112.CrossRefGoogle Scholar
BECKER, W.A. and BEARSE, G.E. (1958) Pre-incubation warming and hatchability of chicken eggs. Poultry Science 37: 944-948.Google Scholar
BECKER, W.A., SPENCER, J.V. and SWARTWOOD, J.L. (1968) Carbon dioxide during storage of chicken and turkey hatching eggs. Poultry Science 47:251-258.CrossRefGoogle ScholarPubMed
BENTON, C.E. and BRAKE, J. (1996) The effect of broiler breeder flock age and length of egg storage on egg albumen during early incubation. Poultry Science 75: 1069-1075.Google Scholar
BERG. L.R., (1945) The relationship of clutch position and time interval between eggs to eggshell quality. Poultry Science 24: 555-563.Google Scholar
BLOOM, S.E., MUSCARELLA, D.E., LEE, M.Y. and RACHLINSKI, M. (1998) Cell death in the avian blastoderm: resistance to stress-induced apoptosis and expression of anti-apoptotic genes. Cell Death Differentiation 5: 529-538.CrossRefGoogle ScholarPubMed
BOWLING, J.A. and HOWARTH, B. (1981) The effects of exposing broiler breeder eggs to high temperatures before storage on hatchability and subsequent performance of chicks. Poultry Science 60: 2333-2336.Google Scholar
BRAKE, J., WALSH, T.J. and VICK, S.V. (1993) Hatchability of broiler eggs as influenced by storage and internal quality. Zootechnica International 16: 30-41.Google Scholar
BRILLARD, J.P. (1993) Sperm storage and transport following natural mating and artificial insemination. Poultry Science 72: 923-928.CrossRefGoogle ScholarPubMed
BRILLARD, J.P. and MCDANIEL, G.R. (1986) Influence of spermatozoa numbers and insemination frequency on fertility in dwarf broiler breeder hens. Poultry Science 65: 2330-2334.CrossRefGoogle ScholarPubMed
BRUGGEMAN, V., WITTERS, A., DE SMIT, L., DEBONNE, M., EVERAERT, N., KAMERS, B., ONAGBESAN, O.M., DEGRAEVE, P. and DECUYPERE, E. (2007) Acid-base balance in chicken (Gallus domesticus) incubated under high CO2 concentrations during the first 10 days of incubation. Respiration Physiology and Neurobiology 159: 147-154.CrossRefGoogle ScholarPubMed
BURLEY, R.W. and VADEHRA, D.V. (1989) The avian egg, chemistry and biology, pp. 472 (New York, John Wiley and Sons).Google Scholar
COLEMAN, J.W. and SIEGEL, P.B. (1966) Selection of body weight at eight weeks of age. 5. Embryonic state at oviposition and its relationship to hatchability. Poultry Science 45: 1003-1011.CrossRefGoogle Scholar
DE SMIT, L., BRUGGEMAN, V., TONA, J.K., DEBONNE, M., ONAGBESAN, O., ARCKENS, L., DE BAERDEMAEKER, J. and DECUYPERE, E. (2006) Embryonic developmental plasticity of the chick: increased CO2 during early stages of incubation changes the developmental trajectories during prenatal and postnatal growth. Comparative Biochemistry and Physiology A, Molecular and Intergrative Physiology 145: 166-175.Google Scholar
EDWARDS, C.L. (1902) The physiological zero and the index of development for the eggs of the domestic fowl Gallus domesticus. American Journal of Physiology 6: 351-396.CrossRefGoogle Scholar
EYAL-GILADI, H. (1984) The gradual establishment of cell commitments during the early stages of chick development. Cell Differentiation 14: 245-255.Google Scholar
EYAL-GILADI, H. (1991) The early embryonic development of the chick as an epigenetic process. Critical Review of Poultry Biology 3: 143-166.Google Scholar
EYAL-GILADI, H. and KOCHAV, S. (1976) From cleavage to primitive streak formation: A complementary normal table and a new look at the first stages of development of the chick. I. General morphology. Developmental Biology 49: 321-337.Google Scholar
FASENKO, G.M., HARDIN, R.T. and ROBINSON, F.E. (1992a) Relationship of hen age and egg sequence position with fertility, hatchability, viability, and pre-incubation embryonic development in broiler breeders. Poultry Science 71: 1374-1383.CrossRefGoogle Scholar
FASENKO, G.M., ROBINSON, F.E. and HARDIN, R.T. (1992b) Research Note: variability in pre-incubation embryonic development in domestic fowl. 2. Effect of duration of egg storage period. Poultry Science 71: 2129-2132.Google Scholar
FASENKO, G.M., WILSON, J.L., ROBINSON, F.E. and HARDIN, R.T. (1999) Effects of length of egg nest holding time and high environmental temperatures on pre-storage embryonic development, survival, and hatchability of broiler breeders. Journal of Applied Poultry Research 8: 488-492.CrossRefGoogle Scholar
FASENKO, G.M., CHRISTENSEN, V.L., WINELAND, M.J. and PETITE, J.N. (2001a) Examining the effects of pre-storage incubation of turkey breeder eggs on embryonic development and hatchability of eggs stored for four to fourteen days. Poultry Science 80: 132-138.Google Scholar
FASENKO, G.M., ROBINSON, F.E., WHELAN, A.I., KREMENIUK, K.M. and WALKER, J.A. (2001b) Pre-storage incubation of long-term stored broiler breeder eggs: 1. Effects on hatchability. Poultry Science 80: 1406-1411.CrossRefGoogle Scholar
FROMM, D. (1964) Strength distribution, weight and some histological aspects of the vitelline membrane of the hen's egg yolk. Poultry Science 43: 1240-1246.Google Scholar
FROMM, D. (1966) The influence of ambient pH on moisture content and yolk index of the hen's yolk. Poultry Science 45: 374-379.CrossRefGoogle Scholar
FUNK, E.M. and BELLIER, H.V. (1944) The minimum temperature for embryonic development in the domestic fowl. Poultry Science 23: 538-540.CrossRefGoogle Scholar
FUNK, E.M. and FORWARD, J. (1960) Effect of holding temperature on hatchability of chicken eggs. Missouri agricultural experiment station bulletin 732: 3-12.Google Scholar
GILLESPIE, J.I. and MCHANWELL, S. (1987) Measurement of intra-embryo pH during early stages of development in the chick embryo. Cell and Tissue Research 247: 445-451.CrossRefGoogle Scholar
GOODRUM, J.W., BRITTON, W.M. and DAVIS, J.B. (1989) Effect of storage conditions on albumen pH and subsequent hard-cooked egg peelability and albumen shear strength. Poultry Science 68: 1226-1231.CrossRefGoogle Scholar
GUPTA, S.K. and BAKST, M.R. (1993) Turkey embryo staging from cleavage through hypoblast formation. Journal of Morphology 217: 313-325.CrossRefGoogle ScholarPubMed
HAYS, F.A. and NICOLAIDES, C. (1934) Variability in development of fresh-laid hen eggs. Poultry Science 13: 74-90.Google Scholar
HOWARTH, B. Jr and and DIGBY, S.T. (1973) Evidence for the penetration of the vitelline membrane of the hen's ovum by a trypsin-like acrosomal enzyme. Journal of Reproduction and Fertility 33: 123-125.Google Scholar
HURNIK, G.I., REINHART, B.S. and HURNIK, J.F. (1978) Relationship between albumen quality and hatchability in fresh and stored eggs. Poultry Science 57: 854-857.Google Scholar
JANSONIUS, F.A.T., PUTIRULAN, F.F. and KALTOFEN, R.S. (1976) De delingsaktiviteit in het blastoderm van een aantal broedeieren, afkomstig van White Plymouth Rock hennen onmiddelijk na leg bepaald. Intern rapport pluimveeonderzoek “Het Spelderholt”, Beekbergen.Google Scholar
KAUFMAN, L. (1938) Entwicklung und Wachstum von Hühnerembryonen in frischen und in gelagerten Eiern. Archiv für Geflügelkunde 13: 63-77.Google Scholar
KOSIN, I.L. and ARORA, K.L. (1966) The pattern of early embryonic development in two genetically isolated lines of Broad Breasted Bronze turkeys. Poultry Science 45: 622-629.Google Scholar
LAPAO, C., GAMA, L.T. and CHAVEIRO SOARES, M. (1999) Effects of broiler breeder age and length of egg storage on albumen characteristics and hatchability. Poultry Science 78: 640-645.Google Scholar
LODGE, J.R., FECHHEIMER, N.S. and JAAP, R.G. (1971) The relationship of in vivo sperm storage interval to fertility and embryonic survival in the chicken. Biology of Reproduction 5: 252-257.Google Scholar
LOURENS, A. (2006) Heating eggs before storage increases hatchability. World Poultry 22(4): 22-23.Google Scholar
LOURENS, A., VAN DEN BRAND, H., MEIJERHOF, R. and KEMP, B. (2005) Effect of eggshell temperature during incubation on embryonic development, hatchability and posthatch development. Poultry Science, 84: 914-920.Google Scholar
MAYES, F.J. and TAKEBALLI, M.A. (1984) Storage of eggs of the fowl (Gallus domesticus) before incubation: a review. World's Poultry Science Journal 40: 131-140.CrossRefGoogle Scholar
MATHER, C.M. and LAUGHLIN, K.F. (1976) Storage of hatching eggs: the effect on total incubation period. British Poultry Science 17: 471-479.CrossRefGoogle Scholar
MCKERLEY, R.G., NEWELL, G.W., BERRY, J.G., ODELL, G.V. and MORRISON, R.D. (1967) The effects of some acidic and alkaline atmospheres on the changes in pH and haugh units in chicken eggs. Poultry Science 46: 118-132.Google Scholar
MCNALLY, E.H. (1943) Some characteristics of the ovomucin gel of egg white. Poultry Science 22: 25-29.CrossRefGoogle Scholar
MEIJERHOF, R. (1994) Theoretical and empirical studies on temperature and moisture loss of hatching eggs during the pre-incubation period. Ph. D. Thesis, Landbouwuniversiteit Wageningen.Google Scholar
MEIJERHOF, R., NOORDHUIZEN, J.P.T.M. and LEENSTRA, F.R. (1994) Influence of pre-incubation treatment on hatching results of broiler breeder eggs produced at 37 and 59 weeks of age. British Poultry Science 35: 249-257.CrossRefGoogle ScholarPubMed
NALBANDOV, A. and CARD, L.E. (1943) Effect of stale sperm on fertility and hatchability of chicken eggs. Poultry Science 22: 218-226.CrossRefGoogle Scholar
NECHAEVA, M.V., TOHARDT, H., HUHNKE, A., MAKARENKO, I.G. and TURPAEV, T.M. (2004) Effects of environmental factors on the amnion rhythmic contractions in chick embryogenesis. Avian and Poultry Biology Reviews 15: 137-144.CrossRefGoogle Scholar
OLSON, M.W. and FRAPS, R.M. (1944) Maturation, fertilization and early cleavage of the egg of the domestic turkey. Journal of Morphology 74: 297-309.CrossRefGoogle Scholar
ONAGBESAN, O., BRUGGEMAN, V., DE SMIT, L., DEBONNE, M., WITTERS, A., TONA, K., EVERAERT, N. and DECUYPERE, E. (2007) Gas exchange during storage and incubation of avian eggs: effects on embryogenesis, hatchability, chick quality and post-hatch growth. World's Poultry Science Journal 63: 557-573.CrossRefGoogle Scholar
PROUDFOOT, F.G. (1962) The decline of internal egg quality during storage at 30°F and 70°F among six strains of Leghorns reared in confinement and on range. Poultry Science 41: 98-103.Google Scholar
PROUDFOOT, F.G. (1964) The effects of plastic packaging and other treatments on hatching eggs. Poultry Science 43: 87-95.Google Scholar
PROUDFOOT, F.G. (1965) The effect of film permeability and concentration of nitrogen, oxygen and helium gases on hatching eggs stored in polyethylene and Cryovac bags. Poultry Science 44: 636-644.Google Scholar
PROUDFOOT, F.G. (1966) Hatchability of stored eggs as affected by daily turning during storage and pre-warming and vacuuming eggs enclosed in plastic with nitrogen. Canadian Journal of Animal Science 46: 47-50.Google Scholar
PROUDFOOT, F.G. (1972) Influence of an improved hatching-egg storage method on the subsequent performance of broiler chickens. Canadian Journal of Animal Science 52(2): 303-308.Google Scholar
RADATZ, E., EYAL-GILADI, H. and KUCERA, P. (1987) Patterns of oxygen consumption during establishment of chephalocaudal polarity in the early chick embryo. Journal of Experimental Zoology Supplement 1: 213-218.Google Scholar
REINHART, B.S. and HURNIK, G.I. (1982) Hatching performance of cryovac enclosed hatching eggs stored in high humidity environment. Poultry Science 61: 564-566.CrossRefGoogle ScholarPubMed
RENEMA, R.A., FEDDES, J.J.R., SCHMID, K.L., FORD, M.A. and KOLK, A.R. (2006) Internal egg temperature in response to pre-incubation warming in broiler breeder and turkey eggs. Journal of Applied Poultry Research 15: 1-8.CrossRefGoogle Scholar
ROBINSON, F.E. (2002) Management for control of ovarian development in broiler breeders. Avian Medicine 59: 1-7.Google Scholar
ROBINSON, F.E., HARDIN, R.T., ROBINSON, N.A. and WILLIAMS, B.J. (1991) The influence of egg sequence position on fertility, embryo viability and embryo weight in broiler breeders. Poultry Science 70: 760-765.CrossRefGoogle ScholarPubMed
SADLER, W.W. (1955) Chronological relationship of the disappearance of the vitelline membrane and the closure of the amnio-chorion in avian embryos and its implications. Poultry Science 34: 754-760.Google Scholar
SCOTT, T.A. and SILVERSIDES, F.G. (2000) The effect of storage and strain of hen on egg quality. Poultry Science 79: 1725-1729.CrossRefGoogle ScholarPubMed
SCOTT, H.M. and WARREN, D.C. (1936) Influence of ovulation rate on the tendency of the fowl to produce eggs in clutches. Poultry Science 15: 381-389.Google Scholar
SHENSTONE, F.S. (1968) The gross composition, chemistry and physico-chemical basis of organization of the yolk and white. In: CARTER, T.C. (Ed) Egg quality: a study of the hen's egg, pp. 26-58 (Edinburgh, Oliver and Boyd).Google Scholar
SILVERSIDES, F.G. and SCOTT, T.A. (2001) Effect of storage and layer age on quality of eggs from two lines of hens. Poultry Science 80: 1240-1245.Google Scholar
SPRATT, N.T. and HAAS, H. (1960) Integrative mechanisms in development of the early chick blastoderm. I. Regulative potentiality of separated parts. Journal of Experimental Zoology 145: 97-137.CrossRefGoogle Scholar
STEINKE, L. (1969) Weitere Keimscheibeuntersuchungen an unbebrüteten Hühnereiern. Archiv für Geflügelkunde 33: 133-146.Google Scholar
STEINKE, L. (1972) Keimscheibeuntersuchungen an Hühnereiern unter besonderer Berücksichtigung des Entwicklungszustandes. Archiv für Geflügelkunde 36: 5-10.Google Scholar
STERN, C.D. (1991) The sub-embryonic fluid of the domestic fowl and its relationship to the early development of the embryo. In: TULLET, S.G. (Ed) Avian incubation, pp. 81-90 (London, Butterworth-Heinemann).Google Scholar
TANEJA, G.C. and GOWE, R.S. (1962) Effect of varying doses of undiluted semen on fertility and hatchability in the domestic fowl. Journal of Reproduction and Fertility 4: 161-174.Google Scholar
TONA, K., BAMELIS, F., DE KETELAERE, B., BRUGGEMAN, V., MOREAS, V.M.B., BUYSE, J., ONAGBESAN, O. and DECUYPERE, E. (2003) Effects of egg storage time on spread of hatch, chick quality, and chick juvenile growth. Poultry Science 82: 736-741.CrossRefGoogle ScholarPubMed
WALSH, T.J., RIZK, R.E. and BRAKE, J. (1995) Effects of temperature and carbon dioxide on albumen characteristics, weight loss and early embryonic mortality of long stored hatching eggs. Poultry Science 74: 1403-1410.CrossRefGoogle Scholar
WARREN, D.C. and SCOTT, H.M. (1935a) The time factor in egg formation. Poultry Science 14: 195-207.Google Scholar
WARREN, D.C. and SCOTT, H.M. (1935b) Physiological factors influencing the rate of egg formation in the domestic hen. Journal of Agricultural Research 51: 565-572.Google Scholar
WILLIAMS, K.C. (1992) Some factors affecting albumen quality with particular reference to haugh unit score. World's Poultry Science Journal 48: 5-16.CrossRefGoogle Scholar
WILSON, H.R. (1991) Physiological requirements of the developing embryo: temperature and turning. In: TULLET, S.G. (Ed) Avian Incubation, pp 145-156 (London, Butterworths).Google Scholar
WIT, A.A.C. DE, KRUIP, and Th, A.M. (2001) Bovine cumulus-oocyte-complex-quality is reflected in sensitivity for α-amanitin, oocyte-diameter and developmental capacity. Animal Reproduction Science 65: 51-65.CrossRefGoogle ScholarPubMed
WHITTOW, G.C. (1986) Regulation of body temperature. In: STURKIE, P.D. (Ed) Avian Physiology, 4th edition, pp. 221-268 (New York, Springer-Verlag).Google Scholar