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Effect of Inhibitors and Herbicides on Tricarboxylic Acid Cycle Substrate Oxidation by Isolated Cucumber Mitochondria

Published online by Cambridge University Press:  12 June 2017

Chester L. Foy  and
Don Penner
Chester L. Foy
Affiliation:
Department of Botany, University of California, Davis
Don Penner
Affiliation:
Department of Botany, University of California, Davis
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Abstract and summary

A mitochondrial fraction isolated from cotyledons of etiolated Cucumis sativus L. (Burpee's Improved Long Green) showed respiratory activity roughly comparable to that of other reported plant mitochondrial systems. Published procedures on extraction, centrifugation, and determination of cofactor requirements were modified for this system to obtain maximum activity as measured by Warburg manometry. Succinic dehydrogenase activity in the fraction was inhibited by known inhibitors such as sodium azide, DNP, and hydroxymalonic acid; α-ketoglutarate utilization was inhibited by sodium azide.

Dichlobenil (2,6-dichlorobenzonitrile) consistently increased respiration (up to 162% of the controls, using 1.45 × 10-4 M dichlobenil in a succinate substrate mixture); 2,5-diiodo-4-hydroxybenzonitrile (ioxynil) consistently reduced O2 uptake at 0.75 × 10-6 to 0.75 × 10-3 M concentrations. Picloram (4-amino-3,5,6-trichloropicolinic acid) performed in a manner generally similar to the five following substituted benzoic acids: 2,3,6-trichlorobenzoic acid (2,3,6-TBA); 3-amino-2,5-dichlorobenzoic acid (amiben); 3-nitro-2,5-dichlorobenzoic acid (dinoben); 2-methoxy-3,6-dichlorobenzoic acid (dicamba); and 2-methoxy-3,5,6-trichlorobenzoic acid (tricamba). Using succinate as substrate, stepwise inhibition occurred in the range of 10-5 to 10-3 M herbicide; however, the α-ketoglutarate system was much less sensitive to all compounds. For example, the effects of 10-3 M amiben and dicamba on α-ketoglutarate utilization were essentially negligible. Six other herbicides studied showed either no effect or varying degrees of inhibition, depending upon concentration and substrate employed. Dichlobenil uncoupled oxidative phosphorylation at a 1.45 × 10-4 M concentration.

Type
Research Article
Information
Weeds , Volume 13 , Issue 3 , July 1965 , pp. 226 - 231
Copyright
Copyright © 1965 Weed Science Society of America 

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References

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