Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-25T05:28:48.833Z Has data issue: false hasContentIssue false
  • English
  • Français

The Biosynthesis of N-(3-Carboxy-2,5-Dichlorophenyl)-Glucosylamine in Plant Tissue Sections

Published online by Cambridge University Press:  12 June 2017

D. S. Frear ,
C. R. Swanson  and
R. E. Kadunce
D. S. Frear
Affiliation:
Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Metabolism and Radiation Research Laboratory, State University Station, Fargo, North Dakota
C. R. Swanson
Affiliation:
Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Metabolism and Radiation Research Laboratory, State University Station, Fargo, North Dakota
R. E. Kadunce
Affiliation:
Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Metabolism and Radiation Research Laboratory, State University Station, Fargo, North Dakota
Get access

Abstract

A quantitative colorimetric procedure was developed for the determination of N-glucosyl amiben in tissue sections of etiolated seedlings. N-glucosyl amiben biosynthesis in soybean hypocotyl sections was heat labile, sensitive to freezing and thawing, unaffected under anaerobic conditions, dependent on amiben concentration, and proportional to tissue section concentration. The formation of N-glucosyl amiben in soybean hypocotyl sections was greatest between pH 6.3 and pH 7.3 and was inhibited by NaF, HgCl2, iodoacetate, iodoacetamide, and N-ethylmaleimide but not by NaCN or NaN3. On a specific activity basis, sugar beet seedling, soybean cotyledon, and soybean hypocotyl tissue sections were the most active tissues studied in the biosynthesis of N-glucosyl amiben.

Type
Research Article
Information
Weeds , Volume 15 , Issue 2 , April 1967 , pp. 101 - 104
Copyright
Copyright © 1967 Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Baker, R. S. and Warren, G. F. 1962. Selective herbicidal action of amiben on cucumber and squash. Weeds 10:219–223.Google Scholar
2. Ballentine, R. 1957. Determination of total nitrogen and ammonia I. Total nitrogen-microvolumetric method B. pp. 987–991. In: Colowick, S. P. and Kaplan, N. O., eds. Methods in Enzymology. Vol. III. Academic Press, New York.Google Scholar
3. Colby, S. R. 1965. Herbicide metabolism: N-glycoside of amiben isolated from soybean plants. Science 150:619–620.Google Scholar
4. Colby, S. R., Warren, G. F., and Baker, R. S. 1965. Fate of amiben in tomato plants. J. Agr. Food Chem. 12:320–321.CrossRefGoogle Scholar
5. Gomori, G. 1955. Preparation of buffers for use in enzyme studies. pp. 138–146. In: Colowick, S. P. and Kaplan, N. O., eds. Methods in Enzymology. Vol. I. Academic Press, New York.Google Scholar
6. Riden, J. R. and Hopkins, T. R. 1961. Decline and residue studies on 4-chloro-2-butynyl-N-(3-chlorophenyl) carbamate. Agr. Food Chem. 9:47–49.Google Scholar
7. Swanson, C. R., Hodgson, R. H., and Frear, D. S. 1965. A major metabolite of 3-amino-2,5-dichlorobenzoic acid (amiben) in plants. Plant Physiol. 40 (suppl.) xiv.Google Scholar
8. Swanson, C. R., Hodgson, R. H., Kadunce, R. E., and Swanson, H. R. 1966. Amiben metabolism in plants. II. Physiological factors in N-glucosyl amiben formation. Weeds (In Press).CrossRefGoogle Scholar
9. Swanson, C. R., Kadunce, R. E., Hodgson, R. H., and Frear, D. S. 1966. Amiben metabolism in plants. I. Isolation and identifications of an N-glucosyl complex. Weeds (In press).CrossRefGoogle Scholar