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Using Electrolyte Leakage to Detect Soybean (Glycine max) Cultivars Sensitive to Sulfentrazone

Published online by Cambridge University Press:  20 January 2017

Zhaohu Li ,
Robert H. Walker ,
Glenn Wehtje  and
H. Gary Hancock
Zhaohu Li
Affiliation:
Department of Agronomy and Soils, Alabama Agricultural Experiment Station, Auburn University, Auburn, AL 36849-5412
Robert H. Walker*
Affiliation:
Department of Agronomy and Soils, Alabama Agricultural Experiment Station, Auburn University, Auburn, AL 36849-5412
Glenn Wehtje
Affiliation:
Department of Agronomy and Soils, Alabama Agricultural Experiment Station, Auburn University, Auburn, AL 36849-5412
H. Gary Hancock
Affiliation:
FMC Corporation, Hamilton, GA 31811
*
Corresponding author's E-mail: [email protected].
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Abstract

Laboratory studies were conducted to determine if electrolyte leakage from either leaf tissue, germinating seeds, or excised roots correlated with previously established soil-applied field response of soybean cultivars and target weeds to sulfentrazone. Sulfentrazone-induced electrolyte leakage from leaf tissue of coffee senna (sensitive), sicklepod (tolerant), and soybean cultivars ‘Asgrow 6785’ and ‘Carver’ (sensitive) and ‘Stonewall’ and ‘DPL 3606’ (tolerant) was monitored over time. Electrolyte leakage from leaf tissues, caused by 25 ppm (65 μM) sulfentrazone, agreed directly with the known response of these weeds, but response of the four soybean cultivars was equivalent. Furthermore, sulfentrazone-induced electrolyte leakage from leaf tissue of Asgrow 6785 and Stonewall was not affected by sulfentrazone concentration as high as 100 ppm (258 μM) nor by light intensity (4 and 120 μmol/m2/s photosynthetically active radiation). For germinating seeds, sulfentrazone-induced electrolyte leakage was also independent of soybean cultivar. In contrast, electrolyte leakage from excised roots of germinal soybean seedlings did concur directly with the previously established cultivar sensitivity to soil-applied sulfentrazone. Results indicate that electrolyte leakage from excised roots of soybean germinal seedlings can be used to assess cultivar sensitivity to soil-applied sulfentrazone.

Keywords

Sulfentrazone, coffee senna, Cassia occidentalis L. #3 CASOCsicklepod, Senna obtusifolia L. # CASOBsoybean, Glycine max (L.) Merr. ‘Asgrow 6785’, ‘Carver’, ‘Stonewall’, ‘DPL 3606’Herbicide tolerancemembrane leakageProtoxlight intensityIe, index of relative electrolyte leakagePAR, photosynthetically active radiationProtogen, protoporphyrinogenProto IX, protoporphyrinProtox, protoporphyrinogen oxidase
Type
Research Article
Information
Weed Technology , Volume 14 , Issue 4 , December 2000 , pp. 699 - 704
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Bewley, J. D. 1979. Physiological aspects of desiccation tolerance. Ann. Rev. Plant Physiol. 30: 195–238.CrossRefGoogle Scholar
Dayan, F. E., Weete, J. D., and Hancock, H. G. 1996. Differential sensitivity to sulfentrazone by sicklepod (Senna obtusifolia) and coffee senna (Cassia occidentalis). Weed Sci. 44: 12–17.CrossRefGoogle Scholar
Dayan, F. E., Weete, J. D., Duke, S. O., and Hancock, H. G. 1997. Soybean (Glycine max) cultivar differences in response to sulfentrazone. Weed Sci. 45: 634–641.Google Scholar
Duke, S. H., Kakefuda, G., and Harvey, T. M. 1983. Differential leakage of intracellular substances from imbibing soybean seeds. Plant Physiol. 72: 919–924.CrossRefGoogle ScholarPubMed
Duke, S. O., Lydon, J., Becerril, J. M., Sherman, T. D., Lehner, L. P. Jr., and Matsumoto, H. 1991. Protoporphyrinogen oxidase-inhibiting herbicides. Weed Sci. 39: 465–473.CrossRefGoogle Scholar
Hancock, H. G. 1992. Weed spectrum of F6285 in soybean. Proc. South. Weed Sci. Soc. 45:49.Google Scholar
Hancock, H. G. 1994. Post-emergent activity of F6285 in soybeans. Proc. South. Weed Sci. Soc. 47:63.Google Scholar
Huang, B. R., Duncan, R. R., and Carrow, R. N. 1997. Drought-resistance mechanisms of seven warm-season turfgrasses under surface soil drying: II. Root aspects. Crop Sci. 37: 1863–1869.Google Scholar
Kenyon, W. H., Duke, S. O., and Vaughn, K. C. 1985. Sequence of herbicidal effects of acifluorfen on ultrastructure and physiology of cucumber cotyledons. Pestic. Biochem. Physiol. 24: 240–250.Google Scholar
Koo, S. J., Neal, J. C., and Tomaso, J. M. 1994. Quinclorac-induced electrolyte leakage in seedling grasses. Weed Sci. 42: 1–7.Google Scholar
Li, Z., Walker, R. H., and Wehtje, G. R. 1999. Use of seedling growth parameters to classify soybean (Glycine max) cultivar sensitivity to sulfentrazone. Weed Technol. 13: 530–535.Google Scholar
Martin, V., Pallardy, S. G., and Bahari, Z. A. 1987. Dehydration tolerance of leaf tissue of six woody angiosperm species. Physiol. Plant. 669: 182–186.Google Scholar
Nandihalli, U. B. and Duke, S. O. 1993. The porphyrin pathway as a herbicide target site. In Duke, S. O., Menn, J. J., and Plimmer, J. R., eds. Pest Control With Enhanced Environmental Safety. American Chemical Society Symposium Series 524. pp. 62–78.CrossRefGoogle Scholar
Vanstone, D. E. and Stobbe, E. H. 1977. Electrolyte conductivity—a rapid measure of herbicide injury. Weed Sci. 25: 352–354.Google Scholar
Vidrine, P. R., Griffin, J. S., Jordan, D. L., and Reynolds, D. B. 1996. Broadleaf weed control in soybean (Glycine max) with sulfentrazone. Weed Technol. 10: 762–765.Google Scholar
Walker, R. H. 1994. F-6285 applied postemergence in soybean. Proc. South. Weed Sci. Soc. 47:64.Google Scholar
Walker, R. H., Richburg, J. S., and Jones, R. E. 1992. F6285 efficacy as affected by rate and method of application. Proc. South. Weed Sci. Soc. 45:51.Google Scholar
Wehtje, G., Walker, R. H., Grey, T. L., and Spratlin, C. E. 1995. Soil effects of sulfentrazone. Proc. South. Weed Sci. Soc. 48: 224–225.Google Scholar
Yaklich, R. W., Kulik, M. M., and Anderson, J. D. 1979. Evaluation of vigor tests in soybean seeds: relationship of ATP, conductivity, and radioactive tracer multiple criteria laboratory tests to field performance. Crop Sci. 19: 806–810.Google Scholar
Yanase, D., Andoh, A., and Yasudomi, N. 1990. A new simple bioassay to evaluate photosynthetic electron-transport inhibition utilizing paraquat phytotoxicity. Pestic. Biochem. Physiol. 35: 92–98.Google Scholar