Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-26T09:06:18.595Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparisons Between X-ray Film- and Phosphorescence Imaging-Based Autoradiography for the Visualization of Herbicide Translocation

Published online by Cambridge University Press:  20 January 2017

Glenn Wehtje ,
Michael E. Miller ,
Timothy L. Grey  and
William R. Brawner Jr
Glenn Wehtje*
Affiliation:
Agronomy and Soils, Auburn University, Auburn, AL 36849
Michael E. Miller
Affiliation:
Department of Biological Sciences, Auburn University, Auburn, AL 36849
Timothy L. Grey
Affiliation:
Crop and Soil Science, University of Georgia, Tifton, GA 31794
William R. Brawner Jr
Affiliation:
Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL 36849
*
Corresponding author's E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Autoradiography is a radioisotope-based technique that allows absorbed and translocated herbicide to be visualized. Autoradiographs are traditionally produced with X-ray film and exposure times of several weeks. Phosphorescence imaging (PI) was investigated as an alternative autoradiography procedure. Smallflower morningglory plants were root-exposed to a series of 14C-atrazine concentrations, producing a series of increasing foliar radioactivity concentrations (i.e., dosage) that ranged from marginal to excessive with respect to autoradiography. Autoradiographs were subsequently produced from these 14C-atrazine-dosed plants using both the X-ray film and the PI technique. Autoradiographs from both techniques were of excellent quality and nearly identical when the dosage was ∼20 to 70 Bq/mg. However, PI produces an acceptable image in dosages either above or below this optimum range. A 1-d exposure time was sufficient with PI, and longer exposure times were not detrimental to image quality. In contrast, a 3-wk exposure time was required with X-ray film. Autoradiographs of selected herbicides are presented to further demonstrate the utility of PI.

Keywords

Atrazinesmallflower morningglory, Jacquemontia tamnifolia (L.) Griseb IAQTADigital imagingradioisotopes
Type
Teaching/Education
Information
Weed Technology , Volume 21 , Issue 4 , December 2007 , pp. 1109 - 1114
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Anderson, O. 1958. Studies on the absorption and translocation of amitrol (3-amino-1,2,4-triazole) by nut grass (Cyperus roundus L.). Weeds 6:370–385.Google Scholar
Crafts, A. S. 1960. Evidence of hydrolysis of esters of 2,4-D during absorption by plants. Weeds 8:19–25.CrossRefGoogle Scholar
Curry, T. S. III, Dowdey, J. E., and Murry, R. C. 1990. Photographic characteristics of X-ray film. Pages 148–163. in. Christensen's Physics of Diagnostic Radiology. 4th ed. Malvern, PA Lea & Febiger.Google Scholar
Davis, D. E., Funderburk, H. H. Jr, and Sansing, N. G. 1959. The absorption and translocation of C14-labeled simazine by corn, cotton and cucumber. Weeds 7:300–309.Google Scholar
Dewey, S. A. and Appleby, A. P. 1983. A comparison between glyphosate and assimilate translocation patterns in tall morningglory. Weed Sci. 31:308–314.Google Scholar
Eastin, E. F. 1986. Absorption, translocation, and degradation of herbicides by plants. Pages 89–108. in Camper, N.D. ed. Research Methods in Weed Science. Champaign, IL Southern Weed Science Society.Google Scholar
Eastin, E. F. and Basler, E. 1977. Absorption, translocation, and degradation of herbicides by plants. Pages 89–108. in Truelove, B. ed. Research Methods in Weed Science. Auburn, AL Southern Weed Science Society, Department of Botany and Microbiology, Auburn University.Google Scholar
Fang, S. C. 1958. Absorption, translocation and metabolism of 2,4-D-1-C14 in pea and tomato. Weeds 6:179–186.Google Scholar
Hahn, J. R. and Peterson, J. H. 1954. Translocation of 3-(p-chlorophenyl)-1,-dimethylurea in plants. Weeds 3:177–187.Google Scholar
Hoagland, D. R. and Arnon, D. I. 1950. The water-culture method for growth in plants without soil. Pages 39. California Agricultural Experiment Station Circular no. 347.Google Scholar
Jansen, C., Schuphan, I., and Schmidt, B. 2000. Glufosinate metabolism in excised shoots and leaves of twenty plant species. Weed Sci. 48:319–326.CrossRefGoogle Scholar
Miller, C. S. and Hall, W. C. 1957. Amino triazole salts and amino triazole-5-C14 studies of pigment production in cotton. Weeds 5:304–315.Google Scholar
Radwan, M. A., Stocking, C. R., and Currier, H. B. 1960. Histoautoradiographic studies of Herbicidal translocation. Weeds 8:657–665.Google Scholar
Rogers, B. J. 1957. Translocation and fate of amino triazole in plants. Weeds 5:5–11.Google Scholar
Sheets, T. J. 1961. Uptake and distribution of simazine by oats and cotton seedlings. Weeds 9:1–13.Google Scholar
W.K. Vencill, ed. 2002. Herbicide Handbook. 8th ed. Lawrence, KS Weed Science Society of America. 493.Google Scholar
Wahlers, R. L., Burton, J. D., Maness, E. P., and Skroch, W. A. 1997. Physiological characteristics of a stem cut and blade delivery method of application. Weed Sci. 45:746–749.Google Scholar
Wathana, S., Corbin, F. T., and Waldrep, T. W. 1972. Absorption and translocation of 2,4-DB in soybean and cocklebur. Weed Sci. 20:120–123.Google Scholar
Williams, M. C., Slife, F. W., and Hanson, J. B. 1960. Absorption and translocation of 2,4-D in several annual and broad leaved weeds. Weeds 8:244–255.Google Scholar
Yamaguchi, S. 1961. Absorption and distribution of EPTC-S35 . Weeds 9:374–380.CrossRefGoogle Scholar
Yamaguchi, S. and Crafts, A. S. 1958. Autoradiographic method for studying absorption and translocation of herbicides using 14C-labeled compounds. Hilgardia 28:161–191.Google Scholar