The U.S. Plant Introduction Collection for six pepper species (2694 accessions) was evaluated for tolerance to bentazon in a field study. Based on injury ratings, Capsicum annuum, C. chinense, and C. frutescens accessions were mostly intermediate in response to bentazon with relatively few highly tolerant or susceptible. Most of C. bacatum, C. chacoense, and C. pubescens and the unspeciated accessions were susceptible. Greenhouse experiments indicated that although most of the tolerant selections were highly tolerant, none was more tolerant than the C. annuum cultivars ‘Santaka’ or ‘Bohemian Chili’. None of the susceptible selections were more susceptible to bentazon than the C. annuum cultivar ‘Sweet Banana’. Individual plants that were highly tolerant were self-pollinated to produce progeny that were all highly bentazon tolerant, but none was more tolerant than Santaka or Bohemian Chili.