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Alternative Herbicides for the Management of Clethodim-Resistant Rigid Ryegrass (Lolium rigidum) in Faba Bean (Vicia faba L.) in Southern Australia

Published online by Cambridge University Press:  20 January 2017

Rupinder Kaur Saini*
Affiliation:
School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia 5064
Samuel G. L. Kleemann
Affiliation:
School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia 5064
Christopher Preston
Affiliation:
School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia 5064
Gurjeet S. Gill
Affiliation:
School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia 5064
*
Corresponding author's E-mail: [email protected].

Abstract

Two field experiments were conducted during 2012 and 2013 at Roseworthy, South Australia to identify effective herbicide options for the management of clethodim-resistant rigid ryegrass in faba bean. Dose–response experiments confirmed resistance in both field populations (B3, 2012 and E2, 2013) to clethodim and butroxydim. Sequencing of the target site of acetyl coenzyme A carboxylase gene in both populations identified an aspartate-2078-glycine mutation. Although resistance of B3 and E2 populations to clethodim was similar (16.5- and 21.4-fold more resistant than the susceptible control SLR4), the B3 population was much more resistant to butroxydim (7.13-fold) than E2 (2.24-fold). Addition of butroxydim to clethodim reduced rigid ryegrass plant density 60 to 80% and seed production 71 to 88% compared with the standard grower practice of simazine PPI plus clethodim POST. Clethodim + butroxydim combination had the highest grain yield of faba bean (980 to 2,400 kg ha−1). Although propyzamide and pyroxasulfone plus triallate PPI provided the next highest levels of rigid ryegrass control (< 60%), these treatments were more variable and unable to reduce seed production (6,354 to 13,570 seeds m−2) to levels acceptable for continuous cropping systems.

En 2012 y 2013, se realizaron dos experimentos de campo en Roseworthy, en el sur de Australia, para identificar opciones de herbicidas efectivos para el manejo de Lolium rigidum resistente a clethodim en campos de haba. Experimentos de respuesta a dosis confirmaron la presencia de resistencia a clethodim y butroxydim en ambas poblaciones de campo (B3, 2012 y E2, 2013). La secuenciación del sitio activo del gen de acetyl coenzyme A carboxylase identificó la mutación aspartate-2078-glycine en ambas poblaciones. Aunque la resistencia a clethodim de B3 y E2 fue similar (16.5 y 21.4 veces más resistentes que el control susceptible SLR4), la población B3 fue mucho más resistente a butroxydim (7.13 veces) que E2 (2.24 veces). La adición de butroxydim a clethodim redujo la densidad de L. rigidum 60 a 80% y la producción de semilla 71 a 88%, al compararse con la práctica estándar de los productores de aplicar simazine PPI más clethodim POST. La combinación de clethodim + butroxydim tuvo el mayor rendimiento de grano de haba (980 a 2,400 kg ha−1). Aunque propyzamide y pyroxasulfone más triallate PPI brindaron los segundos niveles de control de L. rigidum más altos (< 60%), estos tratamientos fueron más variables e incapaces de reducir la producción de semillas (6,354 a 13,570 semillas m−2) a niveles aceptables para sistemas de cultivo continuo.

Type
Research Article
Copyright
Copyright © Weed Science Society of America 

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