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In Vitro Culture of Croftonweed (Ageratina adenophora): Considerable Potential for Fast and Convenient Plantlet Production

Published online by Cambridge University Press:  20 January 2017

Jinbo Shen ,
Xia Li ,
Dandan Wang  and
Hongfei Lu
Jinbo Shen
Affiliation:
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China
Xia Li
Affiliation:
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China
Dandan Wang
Affiliation:
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China
Hongfei Lu*
Affiliation:
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China
*
Corresponding author's E-mail: [email protected]
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Abstract

A callus induction and plantlet regeneration system for croftonweed was developed by studying the influence of explant type (leaf, stem, and nodal segment) and different concentrations of plant growth regulators. The leaf was a better explant for callogenesis compared to the stem. The highest callus induction frequency (87.2%) was obtained from leaf segments on Murashige and Skoog's medium (MS medium) supplemented with 0.5 mg/L (2,4-dichlorophenoxy)acetic acid and 2.0 mg/L 6-benzylaminopurine (BA), and 71.6% differentiation along with a multiplication rate of 4.1 adventitious shoots per callus was achieved with a combination of 0.5 mg/L 1-naphthaleneacetic acid (NAA) and 1.0 mg/L BA. In addition, MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. Rhizogenesis of cultured shoots was satisfactorily obtained in half-strength MS without any growth regulators. The regenerated rooted plantlets were successfully acclimatized in soil where they grew normally without showing any morphological variation. These studies provide the prerequisite system for the development of genetic engineering in the future and propagating croftonweed rapidly for further study.

Keywords

CroftonweedAgeratina adenophora (Spreng.) King & H. E. Robins. EUPADCalli formationplant regenerationtissue culture technologymicropropagation
Type
Research
Information
Weed Technology , Volume 21 , Issue 2 , June 2007 , pp. 445 - 452
Copyright
Copyright © Weed Science Society of America 

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