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Immunoassay to Detect Enhanced Carbamothioate Degradation in Soil

Published online by Cambridge University Press:  12 June 2017

Pamela J. Hutchinson ,
Patrick J. Shea ,
James M. Takacs  and
Paul E. Staswick
Pamela J. Hutchinson
Affiliation:
Dep. Agron., Chem. Dep., and Dep. Agron., Univ. Nebr., Lincoln, NE 68583-0915
Patrick J. Shea
Affiliation:
Dep. Agron., Chem. Dep., and Dep. Agron., Univ. Nebr., Lincoln, NE 68583-0915
James M. Takacs
Affiliation:
Dep. Agron., Chem. Dep., and Dep. Agron., Univ. Nebr., Lincoln, NE 68583-0915
Paul E. Staswick
Affiliation:
Dep. Agron., Chem. Dep., and Dep. Agron., Univ. Nebr., Lincoln, NE 68583-0915
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Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) for EPTC was developed that may be useful for predicting enhanced carbamothioate herbicide degradation in adapted soils. A modified radioimmunoassay (RIA) procedure was used to detect antibodies specific for EPTC and establish antibody dilutions for optimum ELISA sensitivity. Antibodies were obtained from a rabbit injected with a butylate-analog protein conjugate. An indirect ELISA subsequently was developed for EPTC with a detection range of 10 to 1280 ng ml−1 in a 1:3 aqueous dilution of a 90:10 (v/v) acetonitrile (ACN):water soil extract. EPTC recovery from soil was > 96%, as determined by conventional gas chromatography. Measurement of low concentrations of residual EPTC with the ELISA was less quantitative than by gas chromatography, but the immunoassay provided a rapid, potentially cost-effective method for screening soils to determine enhanced carbamothioate degradation.

Keywords

Butylate, S-ethyl bis(2-methylpropyl)carbamothioateEPTC, S-ethyl dipropyl carbamothioateEnzyme-linked immunosorbent assayELISAradioimmunoassayRIAenhanced degradation
Type
Research
Information
Weed Technology , Volume 7 , Issue 2 , June 1993 , pp. 396 - 403
Copyright
Copyright © 1993 Weed Science Society of America 

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