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Use of PCR-based molecular markers to identify weedy Amaranthus species

Published online by Cambridge University Press:  12 June 2017

Denise K. Wetzel ,
Michael J. Horak  and
Daniel Z. Skinner
Denise K. Wetzel
Affiliation:
Department of Agronomy, Kansas State University, Manhattan, KS 66506
Daniel Z. Skinner
Affiliation:
USDA-ARS, Department of Agronomy, Kansas State University, Manhattan, KS 66506
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Abstract

Weedy species of the genus Amaranthus, commonly referred to as pigweeds, have increased in frequency and severity over the past few years. Identification of these weeds is difficult because of similar morphological characteristics among species and variation within species. Studies were initiated to develop a molecular marker identification system utilizing restriction enzyme analysis of amplified ribosomal DNA (rDNA). A set of polymerase chain reaction (PCR) markers was developed to distinguish 10 weedy species of pigweeds. Restriction-site variation, utilizing five endonucleases, within the internal transcribed spacers (ITS) of the rDNA allowed for the positive identification of eight species and one pair of species. These markers will be useful for biological and ecological studies on the genus.

Keywords

Amaranthus spp. AMASSITS1 and ITS2Amaranthus albus L. AMAAL, tumble pigweedAmaranthus arenicola I.M. Johnst. AMAAR, sandhills amaranthAmaranthus blitoides S. Wats. AMABL, prostrate pigweedAmaranthus hybridus L. AMACH, smooth pigweedAmaranthus palmeri S. Wats. AMAPA, Palmer amaranthAmaranthus powellii S. Wats. AMAPO, Powell amaranthAmaranthus retroflexus L. AMARE, redroot pigweedAmaranthus rudis Sauer AMATA, common waterhempAmaranthus spinosus L. AMASPspiny amaranthAmaranthus tuberculatus (Moq.) J.D. Sauer AMATUtall waterhemp
Type
Weed Biology and Ecology
Information
Weed Science , Volume 47 , Issue 5 , October 1999 , pp. 518 - 523
Copyright
Copyright © 1999 by the Weed Science Society of America 

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