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Use of an in Vitro Protein Synthesizing System to Test the Mode of Action of Chloracetamides

Published online by Cambridge University Press:  12 June 2017

Luanne M. Deal ,
J. T. Reeves ,
B. A. Larkins  and
F. D. Hess
Luanne M. Deal
Affiliation:
Dep. Bot. & Plant Pathol., Purdue Univ., West Lafayette, IN 47907
J. T. Reeves
Affiliation:
Dep. Bot. & Plant Pathol., Purdue Univ., West Lafayette, IN 47907
B. A. Larkins
Affiliation:
Dep. Bot. & Plant Pathol., Purdue Univ., West Lafayette, IN 47907
F. D. Hess
Affiliation:
Dep. Bot. & Plant Pathol., Purdue Univ., West Lafayette, IN 47907
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Abstract

The effects of chloracetamides on protein synthesis were studied both in vivo and in vitro. Four chloracetamide herbicides, alachlor [2-chloro-2′,6′-diethyl-N-(methoxymethyl)acetanilide], metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide], CDAA (N–N-diallyl-2-chloroacetamide), and propachlor (2-chloro-N-isopropylacetanilide) were tested for inhibition of [3H]-leucine incorporation into protein. Incorporation of 3H-leucine into trichloroacetic acid (TCA)-insoluble protein was inhibited in oat (Avena sativa L. ‘Victory’) seedlings grown in sand culture and treated 12 h at 1 × 10−4M with these chloracetamides. The herbicides were also tested in a cell-free protein synthesizing system containing polyribosomes purified from oat root cytoplasm. These herbicides had no effect on the rates of polypeptide elongation nor on the synthesis of specific polypeptides when herbicides (1 × 10−4M) were added directly to the system. Polypeptide formation was inhibited 89% when 1 × 10−4M cycloheximide was added during translation. Cytoplasmic polyribosomes were isolated from oat roots treated 12 h with 1 × 10−4M herbicide. Translation rates and products were not altered when these polyribosomes were added to the in vitro system. Protein synthesis is inhibited when tested in an in vivo system; however, the inhibition does not occur during the translation of mRNA into protein.

Keywords

HerbicidealachlorCDAAmetolachlorpropachlor
Type
Research Article
Information
Weed Science , Volume 28 , Issue 3 , May 1980 , pp. 334 - 340
Copyright
Copyright © Weed Science Society of America 

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