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Uptake, Metabolism, and Activity of Haloxyfop in Liquid Cultures of Proso Millet (Panicum miliaceum L. cv Abarr)

Published online by Cambridge University Press:  12 June 2017

Gerard P. Irzyk ,
Thomas T. Bauman  and
Nicholas C. Carpita
Gerard P. Irzyk
Affiliation:
Dep. Bot. and Plant Pathol., Purdue Univ., West Lafayette, IN 47907
Thomas T. Bauman
Affiliation:
Dep. Bot. and Plant Pathol., Purdue Univ., West Lafayette, IN 47907
Nicholas C. Carpita
Affiliation:
Dep. Bot. and Plant Pathol., Purdue Univ., West Lafayette, IN 47907
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Abstract

Suspension cultures of proso millet cells were treated with haloxyfop at different phases of growth. Treatment of 1-d cultures with 1 μM haloxyfop completely inhibited growth within 48 h. In contrast, 1 mM haloxyfop was required to elicit a similar response in 4-, 7-, or 10-d cultures. Calculated IC50 values indicated a 300-fold decrease in haloxyfop sensitivity during the period from 1 to 4 d. The observed changes in sensitivity to haloxyfop could not be attributed to changes in cell concentration during culture growth. In both 1-d and 4-d cultures, an initial rapid uptake of radiolabel was followed by a slow loss of radiolabel from cells. Almost all radioactivity extracted from 1-d and 4-d cells was present as the parent acid. Several radiolabeled compounds in addition to the parent acid were present in media. No major differences in the amounts of these materials were found between 1-d and 4-d media. Our results indicate that a special aspect of metabolism expressed during cell division is particularly sensitive to the herbicide.

Keywords

Proso millet, Panicum miliaceum L. cv. Abarr # PANMIhaloxyfop, 2-[4-[[3-chloro-5-(trifluoromethyl)-2-pyridinyl]oxy]phenoxy]propanoic acidAryloxyphenoxypropionic acidcell divisioncell expansioncell suspension cultureDNAherbicide metabolismherbicide uptakephytotoxicityproteinpyridinyloxyphenoxypropionic acidRNAPANMI
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 38 , Issue 6 , November 1990 , pp. 484 - 491
Copyright
Copyright © 1990 by the Weed Science Society of America 

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