Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-23T05:15:22.897Z Has data issue: false hasContentIssue false
  • English
  • Français

Uncovering Plant Growth-Mediating Allelochemicals Produced by Soil Microorganisms

Published online by Cambridge University Press:  20 January 2017

Sarah M. Carver ,
Nadia Nikulin  and
Jenny Kao-Kniffin
Sarah M. Carver
Affiliation:
Department of Horticulture, School of Integrative Plant Science, Cornell University, 134A Plant Science Building, Ithaca, NY 14853
Nadia Nikulin
Affiliation:
Department of Horticulture, School of Integrative Plant Science, Cornell University, 134A Plant Science Building, Ithaca, NY 14853
Jenny Kao-Kniffin*
Affiliation:
Department of Horticulture, School of Integrative Plant Science, Cornell University, 134A Plant Science Building, Ithaca, NY 14853
*
Corresponding author's email: [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Coevolving interactions between a plant population and its microbiota can potentially yield a rhizosphere enriched in metagenomes containing the blueprints for a vast array of natural products. We describe a method of isolating those metabolites through activity-based screening of soil metagenomic libraries. The method allows for the isolation of small molecules produced in vector–host expression systems containing large-insert DNA fragments extracted from the target plant rhizospheres. Allelopathic activities derived from selected clones were screened against a series of controls. Nonmetric multidimensional scaling (NMS) showed similar effects of the set of controls on lettuce growth, whereas annual bluegrass had a broader range of growth responses. Methanol extracts from clones indicating activity showed distinct patterns in grass seedling growth from the empty vector control, but the same extracts showed no effect on lettuce. The results indicate that the metagenomics method and bioassay screen of clone extracts are tools that can be used for initial determination of allelopathic activity from noncultured soil microbiota.

Keywords

Annual bluegrass, Poa annua L.lettuce, Lactuca sativa L.Allelochemicalbioherbicidemetagenomicsnatural productphytotoxic
Type
Weed Biology and Ecology
Information
Weed Science , Volume 64 , Issue 1 , March 2016 , pp. 119 - 128
Creative Commons
Creative Common License - CCCreative Common License - BY
This is an Open Access article, distributed under the terms of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright
Copyright © Weed Science Society of America

Footnotes

Associate editor for this paper: Franck E. Dayan, USDA-ARS.

References

Literature Cited

Barazani, O, Friedman, J (2001) Allelopathic bacteria and their impact on higher plants. Crit Rev Microbiol 27:41–55 Google Scholar
Benjamini, Y, Hochberg, Y (1995) Controlling the false discovery rate: A practical and powerful approach to multiple testing. J R Stat Soc Ser B Stat Method 57:289–300 Google Scholar
Berendsen, RL, Pieterse, CM, Bakker, PA (2012) The rhizosphere microbiome and plant health. Trends Plant Sci 17:478–486.Google Scholar
Blodgett, JAV, Zhang, JK, Metcalf, WW (2005) Molecular cloning, sequence analysis, and heterologous expression of the phosphinothricin tripeptide biosynthetic gene cluster from Streptomyces viridochromogenes DSM 40736. Antimicrob Agents Chemother 49:230–240 Google Scholar
Brady, SF (2007) Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules. Nat Protoc 2:1297–1305 Google Scholar
Ekkers, DM, Cretoiu, MS, Kielak, AM, van Elsas, JD (2012) The great screen anomaly—a new frontier in product discovery through functional metagenomics. Appl Microbiol Biotechnol 93:1005–1020 Google Scholar
Haas, D, Keel, C (2003) Regulation of antibiotic production in root-colonizing Pseudomonas spp. and relevance for biological control of plant disease. Annu Rev Phytopathol 41:117–153 Google Scholar
Hadar, Y, Papadopoulou, KK (2012) Suppressive composts: microbial ecology links between abiotic environments and healthy plants. Annu Rev Phytopathol 50:133–153 Google Scholar
Henkin, TM, Sonenshein, AL (1987) Mutations of the Escherichia coli lacUV5 promoter resulting in increased expression in Bacillus subtilis . Mol Gen Genet 209:467–474 Google Scholar
Kao-Kniffin, J, Carver, SM, DiTommaso, A (2013) Advancing weed management strategies using metagenomic techniques. Weed Sci 61:171–184 Google Scholar
Kenkel, NC (2006) On selecting an appropriate multivariate analysis. Can J Plant Sci 86:663–676 Google Scholar
Lorenz, P, Eck, J (2005) Metagenomics and industrial applications. Nat Rev Microbiol 3:510–516 Google Scholar
Mazzola, M, Gu, YH (2002) Wheat genotype-specific induction of soil microbial communities suppressive to disease incited by Rhizoctonia solani anastomosis group (AG)-5 and AG-8. Phytopathology 92:1300–1307 Google Scholar
McCune, B, Grace, JB (2002) Analysis of Ecological Communities. Gleneden Beach, OR MjM Google Scholar
Petermann, JS, Fergus, AJ, Turnbull, LA, Schmid, B (2008) Janzen-Connell effects are widespread and strong enough to maintain diversity in grasslands. Ecology 89:2399–2406 Google Scholar
Raaijmakers, JM, Mazzola, M (2012) Diversity and natural functions of antibiotics produced by beneficial and plant pathogenic bacteria. Annu Rev Phytopathol 50:403–424 Google Scholar
Raaijmakers, JM, Vlami, M, De Souza, JT (2002) Antibiotic production by bacterial biocontrol agents. Antonie Leeuwenhoek 81:537–547 Google Scholar
Reynolds, HL, Packer, A, Bever, JD, Clay, K (2003) Grassroots ecology: plant-microbe-soil interactions as drivers of plant community structure and dynamics. Ecology 84:2281–2291 Google Scholar
Rutledge, PJ, Challis, GL (2015) Discovery of microbial natural products by activation of silent biosynthetic gene clusters. Nat Rev Microbiol 13:509–523.Google Scholar
Schloss, PD, Handelsman, J (2003) Biotechnological prospects from metagenomics. Curr Opin Biotechnol 14:303–310 Google Scholar
Strong, SJ, Ohta, Y, Litman, GW, Amemiya, CT (1997) Marked improvement of PAC and BAC cloning is achieved using electroelution of pulsed-field gel-separated partial digestions of genomic DNA. Nucleic Acids Res 25:3959–3961 Google Scholar
Torsvik, V, Goksoyr, J, Daae, FL (1990) High diversity in DNA of soil bacteria. Appl Environ Microbiol 56:782–787.Google Scholar
Waite, TA, Campbell, LG (2006) Controlling the false discovery rate and increasing statistical power in ecological studies. Ecoscience 13:439–442 Google Scholar
Weller, DM, Raaijmakers, JM, Gardener, BBM, Thomashow, LS (2002). Microbial populations responsible for specific soil suppressiveness to plant pathogens 1. Annu Rev Phytopathol 40:309–348 Google Scholar
Zhou, J, Bruns, MA, Tiedje, JM, (1996) DNA recovery from soils of diverse composition. Appl Environ Microbiol 62:316–322 Google Scholar