Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-22T18:53:58.907Z Has data issue: false hasContentIssue false
  • English
  • Français

Toxicity profiles of potential biocontrol agents of Orobanche ramosa

Published online by Cambridge University Press:  20 January 2017

Mohamed A. Abouzeid ,
Angela Boari ,
Maria Chiara Zonno ,
Maurizio Vurro  and
Antonio Evidente
Mohamed A. Abouzeid
Affiliation:
Dipartimento di Scienze del Suolo, della Pianta e dell'Ambiente (DiSSPA), Università di Napoli “Federico II”, Via Università 100, 80055 Portici, Italy
Angela Boari
Affiliation:
Dipartimento di Scienze del Suolo, della Pianta e dell'Ambiente (DiSSPA), Università di Napoli “Federico II”, Via Università 100, 80055 Portici, Italy
Maria Chiara Zonno
Affiliation:
Dipartimento di Scienze del Suolo, della Pianta e dell'Ambiente (DiSSPA), Università di Napoli “Federico II”, Via Università 100, 80055 Portici, Italy
Maurizio Vurro
Affiliation:
Dipartimento di Scienze del Suolo, della Pianta e dell'Ambiente (DiSSPA), Università di Napoli “Federico II”, Via Università 100, 80055 Portici, Italy
Get access Rights & Permissions [Opens in a new window]

Abstract

Fifty-three fungal strains belonging to 15 mainly Fusarium species were isolated from branched broomrape plants. Their virulence was assessed using a plastic bag system, and they were grown both in liquid and on solid media, extracted, and the extracts were chemically analyzed and biologically assayed to find new metabolites that inhibit germination of branched broomrape to estimate the production of fusaric and dehydrofusaric acids by Fusarium strains and evaluate their possible involvement as virulence factors and their practical use as biomarkers to make the selection of potential mycoherbicides easier, and to ascertain whether toxins affected mammals. Nine strains proved to be highly virulent and 18 strains produced fusaric and dehydrofusaric acid at concentrations from 4 to 165, and from 9 to 204 mg L−1 respectively. Fifteen extracts from solid cultures caused high mortality when assayed on brine shrimps. Five extracts from liquid cultures caused total inhibition of seed germination. Some strains may be considered as sources of new biocontrol agents but virulence was not always positively correlated to the production of phytotoxic compounds.

Keywords

Branched broomrape, Orobanche ramosa L.Fusarium acuminatum Ell. & Ev.F. camptoceras Wollenw. & ReinkingF. chlamydosporum Wollenw. & ReinkingF. compactum sensu GordonF. equiseti (Corda) Sacc.F. nygamai Burgess & TrimboliF. oxysporum Schlecht.F. proliferatum Matsushima (Nirenberg)F. sambucinum Fuckel sensu strictoF. solani (Mart.) Appel & Wollenw.F. verticillioides Sacc. (Nirenberg)P. tabacinum (van Beyma) M. E. Palm et al.Phytopathogenic fungitoxic metabolitesbroomrapebioherbicideOrobanche ramosa
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 52 , Issue 3 , June 2004 , pp. 326 - 332
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Abbas, H. K., Tak, H., Boyette, C. D., Shier, W. T., and Jarvis, B. B. 2001. Macrocyclic trichothecenes are undetectable in kudzu (Pueraria montana) plants treated with a high-producing isolate of Myrothecium verrucaria . Phytochemistry 58:269–276.Google Scholar
Amalfitano, C., Pengue, R., Andolfi, A., Vurro, M., Zonno, M. C., and Evidente, A. 2002. HPLC analysis of fusaric acid, 9,10-dehydrofusaric acid and their methyl esters, toxic metabolites produced by weed pathogenic Fusarium species. Phytochem. Anal 13:277–282.Google Scholar
Amsellem, Z., Cohen, B. A., and Gressel, J. 2002. Engineering hypervirulence in a mycoherbicidal fungus for efficient weed control. Nat. Biotechnol 20:1035–1039.Google Scholar
Amsellem, Z., Kleifeld, Y., Kerenyi, Z., Hornok, L., Goldwasser, Y., and Gressel, J. 1996. Isolation of mycoherbicidal pathogens from juvenile broomrape plants. Phytopathology 86:1–13.Google Scholar
Amsellem, Z., Zidack, N. K., Quimby, P. C. Jr., and Gressel, J. 1999. Long- term dry preservation of viable mycelia of two mycoherbicidal organisms. Crop Prot 18:643–649.Google Scholar
Aviv, D., Amsellem, Z., and Gressel, J. 2002. Transformation of carrots with mutant acetolactate synthase for Orobanche (broomrape) control. Plant Sci 58:1187–1193.Google Scholar
Bacon, C. W., Porter, J. K., Norred, W. P., and Leslie, J. F. 1996. Production of fusaric acid by Fusarium species. Appl. Environ. Microbiol 62:4039–4043.CrossRefGoogle ScholarPubMed
Bottalico, A., Capasso, R., Evidente, A., and Vurro, M. 1994. Process for the production and purification of cytochalasin B from Phoma exigua var. heteromorpha . Appl. Biochem. Biotechnol 48:33–36.Google Scholar
Capasso, R., Evidente, A., Cutignano, A., Vurro, M., Zonno, M. C., and Bottalico, A. 1996. Fusaric and 9,10-dehydrofusaric acids and their methyl esters from Fusarium nygamai . Phytochemistry 41:1035–1039.Google Scholar
Chen, T., Kilpatrick, R. A., and Rich, A. E. 1961. Sterile culture techniques as tools in plant nematology research. Phytopathology 51:799–804.Google Scholar
Claydon, N., Grove, J. F., and Pople, M. 1977. Fusaric acid from Fusarium solani . Phytochemistry 16:603.Google Scholar
Cohen, B. A., Amsellem, Z., Lev-Yadun, S., and Gressel, J. 2002. Infection of tubercles of the parasitic weed Orobanche aegyptiaca by mycoherbicidal Fusarium species. Ann. Bot 90:567–578.Google Scholar
Eppley, R. M. 1974. Sensitivity of brine shrimp (Artemia salina) to trichothecenes. J. Assoc. Off. Anal. Chem 57:618–620.Google Scholar
Evidente, A. and Motta, A. 2001. Phytotoxins from fungi, pathogenic for agrarian, forestall and weedy plants. Pages 473–525 in Tringali, C. ed. Bioactive Compounds from Natural Sources. London: Taylor & Francis.Google Scholar
Holm, L., Doll, J., Holm, E., Pancho, J., and Herberger, J. 1997. World Weeds. Natural Histories and Distribution. New York: J. Wiley.Google Scholar
Joel, D. M., Kleifeld, Y., Losner-Goshen, D., Herzlinger, G., and Gressel, J. 1995. Transgenic crops against parasites. Nature 374:220–221.Google Scholar
Kern, H. 1970. Phytotoxins produced by Fusaria. Pages 35–45 in Wood, R.K.S., Ballio, A., and Graniti, A. eds. Phytotoxins in Plant Diseases. New York: Academic.Google Scholar
Mulè, G., Logrieco, A., Stea, G., and Bottalico, A. 1997. Clustering of trichothecene-producing Fusarium strains determined from 28S ribosomal DNA sequences. Appl. Envir. Microbiol 63:1843–1846.Google Scholar
Parker, C. and Dixon, N. 1983. The use of polyethylene bags in the culture and study of Striga spp. and other organisms on crop roots. Ann. Appl. Biol 103:481–488.Google Scholar
Pinkerton, F. and Strobel, G. A. 1976. Serinol as an activator of toxin production in attenuated cultures of Helminthosporium sacchari . Proc. Natl. Acad. Sci. USA 73:4007–4011.Google Scholar
Strobel, G. A., Kenfield, D., Bunkers, G., Sugawara, F., and Clardy, J. 1991. Phytotoxins as potential herbicides. Experientia 47:819–826.Google Scholar
Surov, T., Aviv, D., Aly, R., Joel, D. M., Goldman-Guez, T., and Gressel, J. 1997. Generation of transgenic asulam-resistant potatoes to facilitate eradication of parasitic broomrapes (Orobanche spp.), with the sul gene as the selectable marker. Theor. Appl. Genet 96:132–137.CrossRefGoogle Scholar
Vurro, M. 2001. Microbial toxins in biocontrol enhancement strategies. Pages 28–37 in Vurro, M. et al. eds. Enhancing Biocontrol Agents and Handling Risks. NATO Science Series. Amsterdam: IOS.Google Scholar
Zonno, M. C. and Vurro, M. 1999. Effect of fungal toxins on germination of Striga hermonthica seeds. Weed Res 39:15–20.CrossRefGoogle Scholar
Zonno, M. C. and Vurro, M. 2002. Inhibition of germination of Orobanche ramosa seeds by Fusarium toxins. Phytoparasitica 30:519–524.CrossRefGoogle Scholar