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Thiocarbamate Inhibition of Fatty Acid Biosynthesis in Isolated Spinach Chloroplasts

Published online by Cambridge University Press:  12 June 2017

R. E. Wilkinson  and
A. E. Smith
R. E. Wilkinson
Affiliation:
Dep. of Agron., Georgia State, Experiment, GA 30212
A. E. Smith
Affiliation:
Dep. of Agron., Georgia State, Experiment, GA 30212
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Abstract

EPTC (S-ethyl dipropylthiocarbamate) (33 μM) and diallate [S-(2,3-dichloroallyl)diisopropylthiocarbamate] (90 μM) inhibited the incorporation of 6 mM acetate-2-14 C (Ac∗) by 80% and 65%, respectively, and the incorporation of 0.5 μM malonate-2-14C (Mal∗) by 32% and 26%, respectively, into the lipids of spinach (Spinacia oleracea L.) chloroplasts. The inhibition of Ac∗ or Mal∗ incorporation into lipids was not observed in the presence of excess Ac∗ or Mal∗, respectively. Incorporation of palmitate-1-14C and oleate-1-14C into chloroplast lipids was inhibited by EPTC and diallate. Mal∗ incorporation into dienoic fatty acids was inhibited by EPTC and diallate. The concentration of EPTC and diallate inhibiting lipid synthesis falls into the physiological range of these herbicides, explains some metabolic effects of these compounds, and fits as the mode of activity of these herbicides.

Type
Research Article
Information
Weed Science , Volume 23 , Issue 2 , March 1975 , pp. 100 - 104
Copyright
Copyright © 1975 by the Weed Science Society of America 

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References

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