Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-25T16:05:05.014Z Has data issue: false hasContentIssue false
  • English
  • Français

Target-site resistance to acetolactate synthase inhibitors in wild mustard (Sinapis arvensis)

Published online by Cambridge University Press:  20 January 2017

Michael J. Christoffers ,
Vijay K. Nandula ,
Kirk A. Howatt  and
Todd R. Wehking
Vijay K. Nandula
Affiliation:
Department of Plant Sciences, North Dakota State University, Fargo, ND 58105
Kirk A. Howatt
Affiliation:
Department of Plant Sciences, North Dakota State University, Fargo, ND 58105
Todd R. Wehking
Affiliation:
Department of Plant Sciences, North Dakota State University, Fargo, ND 58105
Get access Rights & Permissions [Opens in a new window]

Abstract

Inhibitors of acetolactate synthase (ALS) are important herbicides for control of wild mustard, a common weed of the north central United States and Canada. Wild mustard that survived treatments with the ALS inhibitors cloransulam, imazethapyr, and thifensulfuron was sampled from a North Dakota soybean field in 1999. The mechanism of resistance and response of this wild mustard biotype to ALS-inhibiting herbicides was investigated. In vitro enzyme-inhibition experiments confirmed a resistance mechanism associated with the ALS enzyme; imazethapyr or imazamox at 1 × 10−4 M caused only 10 to 11% and 12 to 16% reductions in ALS activity, respectively. ALS from a susceptible wild mustard biotype was inhibited 50% (I50) with imazethapyr at 8 × 10−7 M or imazamox at 1.1 × 10−6 M. Whole-plant greenhouse treatments confirmed cross-resistance across ALS-inhibitor classes. Treatment with twice-normal field rates of thifensulfuron, ethametsulfuron, triflusulfuron, imazamox, imazethapyr, flumetsulam, cloransulam, flucarbazone, and imazamethabenz reduced biomass of the susceptible biotype at least 96% 28 d after treatment. Biomass of the resistant biotype was reduced 49% by triflusulfuron and 35% by thifensulfuron, but was not reduced by other herbicides. DNA sequence analysis of ALS genes from resistant and susceptible biotypes revealed a point mutation inferring a Trp-to-Leu amino acid substitution in ALS of the resistant biotype. This mutation, corresponding to position 574 of the Arabidopsis ALS amino acid sequence, is known to confer cross-resistance to ALS-inhibiting herbicides and is the probable cause of resistance in the wild mustard biotype. Phylogenetic analysis of wild mustard and canola ALS sequences confirmed that the Trp574 mutation arose within wild mustard and was not derived via introgression from imidazolinone-resistant canola. The results of this research indicate a naturally occurring target-site point mutation responsible for conferring cross-resistance to ALS-inhibiting herbicides in this wild mustard biotype.

Keywords

Cloransulam, ethametsulfuron, flucarbazone, flumetsulam, imazamethabenz, imazamox, imazethapyr, thifensulfuron, triflusulfuron, Arabidopsis, Arabidopsis thaliana (L.) Heynh., ARBTHwild mustard, Sinapis arvensis L., SINARcanola, Brassica napus L.soybean, Glycine max (L.) MerrAcetohydroxyacid synthaseALS sequencesAHASherbicide resistancetarget-site mutation
Type
Physiology, Chemistry, Biochemistry
Information
Weed Science , Volume 54 , Issue 2 , April 2006 , pp. 191 - 197
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Current address: Delta Research and Extension Center, Mississippi State University, Stoneville, MS 38776
Current address: Cargill Inc., Minneapolis, MN 55440

References

Literature Cited

Bekkaoui, F., Condie, J. A., Neustaedter, D. A., Moloney, M. M., and Crosby, W. L. 1991. Isolation, structure and expression of a cDNA for acetolactate synthase from Brassica napus . Plant Mol. Biol 16:741–744.CrossRefGoogle ScholarPubMed
Berglund, D. R. 2000. 1999 Canola Variety Trials. Fargo, ND: North Dakota State University Extension Service. 22 p.Google Scholar
Bing, D. J., Downey, R. K., and Rakow, G. F. W. 1996. Hybridizations among Brassica napus, B. rapa and B. juncea and their two weedy relatives B. nigra and Sinapis arvensis under open pollination conditions in the field. Plant Breed. 115:470–473.CrossRefGoogle Scholar
Duggleby, R. G. and Pang, S. S. 2000. Acetohydroxyacid synthase. J. Biochem. Mol. Biol 33:1–36.Google Scholar
Eber, F., Chèvre, A. M., Baranger, A., Vallée, P., Tanguy, X., and Renard, M. 1994. Spontaneous hybridization between a male-sterile oilseed rape and two weeds. Theor. Appl. Genet 88:362–368.Google Scholar
Fang, L. Y., Gross, P. R., Chen, C-H., and Lillis, M. 1992. Sequence of two acetohydroxyacid synthase genes from Zea mays . Plant Mol. Biol 18:1185–1187.Google Scholar
Felsenstein, J. 1995. PHYLIP (Phylogeny Inference Package). Version 3.57c. Seattle, WA: Department of Genetics, University of Washington.Google Scholar
Heap, I. International Survey of Herbicide Resistant Weeds. www.weedscience.com. Accessed March 14, 2005.Google Scholar
Jeffers, G. M., O'Donovan, J. T., and Hall, L. M. 1996. Wild mustard (Brassica kaber) resistance to ethametsulfuron but not to other herbicides. Weed Technol 10:847–850.Google Scholar
Lefol, E., Danielou, V., and Darmency, H. 1996. Predicting hybridization between transgenic oilseed rape and wild mustard. Field Crops Res 45:153–161.CrossRefGoogle Scholar
Moodie, M., Finch, R. P., and Marshall, G. 1997. Analysis of genetic variation in wild mustard (Sinapis arvensis) using molecular markers. Weed Sci 45:102–107.Google Scholar
Morrison, I. N. and Devine, M. D. 1994. Herbicide resistance in the Canadian prairie provinces: five years after the fact. Phytoprotection 75:(Suppl.). 5–16.Google Scholar
Ouellet, T., Rutledge, R. G., and Miki, B. L. 1992. Members of the acetohydroxyacid synthase multigene family of Brassica napus have divergent patterns of expression. Plant J 2:321–330.Google Scholar
Ray, T. B. 1984. Site of action of chlorsulfuron: inhibition of valine and isoleucine biosynthesis in plants. Plant Physiol 75:827–831.CrossRefGoogle ScholarPubMed
Rutledge, R. G., Quellet, T., Hattori, J., and Miki, B. L. 1991. Molecular characterization and genetic origin of the Brassica napus acetohydroxyacid synthase multigene family. Mol. Gen. Genet 229:31–40.CrossRefGoogle ScholarPubMed
Saari, L. L., Cotterman, J. C., and Thill, D. C. 1994. Resistance to acetolactate synthase inhibiting herbicides. Pages 83–139 in Powles, S. B. and Holtum, J.A.M. eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL: Lewis.Google Scholar
Shaner, D. L., Bascomb, N. F., and Smith, W. 1996. Imidazolinone-resistant crops: selection, characterization, and management. Pages 143–157 in Duke, S. O. ed. Herbicide Resistant Crops. Boca Raton, FL: Lewis.Google Scholar
Tranel, P. J. and Wright, T. R. 2002. Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci 50:700–712.CrossRefGoogle Scholar
Veldhuis, L. J., Hall, L. M., O'Donovan, J. T., Dyer, W., and Hall, J. C. 2000. Metabolism-based resistance of a wild mustard (Sinapis arvensis L.) biotype to ethametsulfuron-methyl. J. Agric. Food Chem 48:2986–2990.CrossRefGoogle ScholarPubMed
Warwick, S. I., Beckie, H. J., Thomas, A. G., and McDonald, T. 2000. The biology of Canadian weeds. 8. Sinapis arvensis L. (updated). Can. J. Plant Sci 80:939–961.CrossRefGoogle Scholar
Warwick, S. I., Sauder, C., and Beckie, H. J. 2005. Resistance to Canadian biotypes of wild mustard (Sinapis arvensis) to acetolatate synthase inhibiting herbicides. Weed Sci 53:631–639.Google Scholar
Warwick, S. I., Simard, M-J., Légère, A., Beckie, H. J., Braun, L., Zhu, B., Mason, P., Séguin-Swartz, G., and Stewart, C. N. 2003. Hybridization between transgenic Brassica napus L. and its wild relatives: Brassica rapa L., Raphanus raphanistrum L., Sinapis arvensis L., and Erucastrum gallicum (Willd.) O.E. Schulz. Theor. Appl. Genet 107:528–539.CrossRefGoogle ScholarPubMed
Whaley, C. M., Wilson, H. P., and Westwood, J. H. 2004. Characterization of a new ALS-inhibitor resistance mutation from the ALS gene of smooth pigweed (Amaranthus hybridus). Abstr. Weed Sci. Soc. Am 44:46.Google Scholar
Wiersma, P. A., Schmiemann, M. G., Condie, J. A., Crosby, W. L., and Moloney, M. M. 1989. Isolation, expression and phylogenetic inheritance of an acetolactate synthase gene from Brassica napus . Mol. Gen. Genet 219:413–420.Google Scholar
Zollinger, R. K., Ries, J. L., and Hammond, J. J. 2003. Survey of Weeds in North Dakota—2000. Fargo, ND: North Dakota State University Extension Service. 97 p.Google Scholar