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Spectrophotometric and Spectrofluorometric Methods in Weed Science

Published online by Cambridge University Press:  12 June 2017

Stephen O. Duke ,
Mary V. Duke ,
Timothy D. Sherman  and
Ujjana B. Nandihalli
Stephen O. Duke
Affiliation:
South. Weed Sci. Lab., Agric. Res. Serv., U.S. Dep. Agric., P.O. Box 350, Stoneville, MS 38776
Mary V. Duke
Affiliation:
South. Weed Sci. Lab., Agric. Res. Serv., U.S. Dep. Agric., P.O. Box 350, Stoneville, MS 38776
Timothy D. Sherman
Affiliation:
South. Weed Sci. Lab., Agric. Res. Serv., U.S. Dep. Agric., P.O. Box 350, Stoneville, MS 38776
Ujjana B. Nandihalli
Affiliation:
South. Weed Sci. Lab., Agric. Res. Serv., U.S. Dep. Agric., P.O. Box 350, Stoneville, MS 38776
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Abstract

The utility, advantages, and disadvantages of several spectrofluorometric and in vivo spectrophotometric methods in plant science are reviewed, with emphasis on their use in weed science. Examples of the use of in vivo, dual-wavelength spectrophotometry to probe the effects of herbicides on cytochrome f oxidation/reduction, the P515 chromatic shift, phytochrome synthesis, and other processes are discussed. Use of in vivo spectrophotometry to examine effects of herbicides on phytylation of chlorophyllide, protochlorophyllide photoconversion, porphyrin accumulation, and other processes that result in spectral changes is discussed and examples are provided. Spectrofluorometric methods for pigment identification and quantification and for enzyme assays are illustrated with examples. Spectrofluorometric methods used in conjunction with high-pressure liquid chromatography (HPLC) and thin-layer chromatography (TLC) are discussed.

Keywords

Acifluorfen, 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acidclomazone, 2-[(2-chlorophenyl)methyl]-4,4-dimethyl-3-isoxazolidinonediuron, N′-(3,4-dichlorophenyl)-N,N-dimethylureanorflurazon, 4-chloro-5-(methylamino)-2-(3-(trifluoromethyl)phenyl)-3 (2H)-pyridazinoneoxadiazon, 3-[2,4-dichloro-5-(1-methylethoxy)phenyl]-5-(1,1-dimethylethyl)-1,3,4-oxadiazol-2-(3H)-oneparaquat, 1,1′-dimethyl-4,4′-bipyridinium ioncowpea, Vigna unguiculata L.johnsongrass, Sorghum halepense (L.) Pers. ♯ SORHAmung bean, Vigna radiata (L.)pea, Pisum sativum L.Acifluorfenchlorophyllcytochrome fgreeningherbicidesP515phytochromeporphyrinsprotoporphyrin IXShibata shift
Type
Special Topics
Information
Weed Science , Volume 39 , Issue 3 , September 1991 , pp. 505 - 513
Copyright
Copyright © 1991 by the Weed Science Society of America 

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References

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