Laboratory and field experiments were conducted to describe the root system of honeyvine milkweed [Cynanchum laeve (Michx.) Pers.], and to study factors affecting the growth of plants from root fragments of shallow, ascending roots. The root system of a typical plant was composed of lateral roots radiating from a sparsely-branched, vertical taproot. Laterals tended to concentrate in a boundary area between a loamy surface soil and a zone of clay accumulation, this boundary area being below the zone of cultivation. Vertical taproots were found to a depth of 2 m, with sections collected at this and shallower depths demonstrating the ability to produce aerial shoots. In laboratory studies, root sections were killed either by freezing for 2 h or by drying at either 20 or 30 C for 24 h. The optimum temperature for shoot development from root sections was between 20 and 30 C. Shoot emergence and growth from buried root sections were generally unaffected by either depth of planting or root length. As many as 45 daughter shoots were produced from a single plant originating from seed, and 27 daughter shoots were developed from a root section 131 days after planting. The maximum distance daughter shoots were observed from original plants 131 days after planting was 111 cm.