Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-22T19:57:21.917Z Has data issue: false hasContentIssue false
  • English
  • Français

Role of Antioxidative System in Paraquat Resistance of Tall Fleabane (Conyza sumatrensis)

Published online by Cambridge University Press:  20 January 2017

Yeong-Jene Chiang ,
Yi-Xuan Wu ,
Mou-Yen Chiang  and
Ching-Yuh Wang
Yeong-Jene Chiang
Affiliation:
Division of Plant Toxicology, Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, 11 Kuangming Road, Wufeng, Taichung County, Taiwan
Yi-Xuan Wu
Affiliation:
Department of Agronomy, National Chung-Hsing University, 250 Kuokuang Road, Taichung City, Taiwan
Mou-Yen Chiang
Affiliation:
Division of Plant Toxicology, Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, 11 Kuangming Road, Wufeng, Taichung County, Taiwan
Ching-Yuh Wang*
Affiliation:
Department of Agronomy, National Chung-Hsing University, 250 Kuokuang Road, Taichung City, Taiwan
*
Corresponding author's E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

In order to explore the physiological mechanism of paraquat resistance in tall fleabane, a widespread weed in Taiwan where resistance to this herbicide has been observed since 1980, the role of the antioxidative system was assessed. The susceptible (S) and resistant (R) biotypes of tall fleabane were distinguished clearly by the relative distribution frequency of injury index caused by 78 µM paraquat. Although malondialdehyde, an indicator for peroxidation damage to the plant, in the the R-biotype was not changed, in the S-biotype malondialdehyde increased within 2 h after treatment of 50 µM paraquat. Analysis of several antioxidants and pertinent enzymes revealed that ascorbate peroxidase activity was decreased by paraquat treatment in the S-biotype; and a lower basal level of ascorbate was present in the S-biotype as well. The maintenance of a high ratio of reduced glutathione to total glutathione, coupled with a pronounced and rapid increase of glutathione reductase (GR) activity in the the R-biotype, suggests that an active reduced glutathione/oxidized glutathione (GSH/GSSG) cycle is critical to paraquat resistance of tall fleabane. The decisive contribution of a functional GSH/GSSG cycle to paraquat resistance through an enhancement of GR activity in this weed was further confirmed by an experiment of exogenous application of ascorbate.

Keywords

Paraquattall fleabaneConyza sumatrensis (Retz.) WalkerResistant biotypeantioxidantglutathione cycle
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 56 , Issue 3 , June 2008 , pp. 350 - 355
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Alizadeh, H. M., Preston, C., and Powles, S. B. 1998. Paraquat-resistance biotypes of Hordeum glaucum from zero-tillage wheat. Weed Res. 38:139–142.CrossRefGoogle Scholar
Bielawski, W. and Joy, K. W. 1986. Reduced and oxidized glutathione and glutathione-reductase activity in tissues of Pisum sativum . Planta. 169:267–272.Google Scholar
Chase, C. A., Bewick, T. A., and Shilling, D. G. 1998. Characterization of paraquat resistance in Solanum americanum Mill. II. Evidence for a chloroplast mechanism. Pestic. Biochem. Physiol. 60:23–30.Google Scholar
Chiang, Y. J. and Chiang, M. Y. 2006. Handbook on Herbicides and Farmland Weeds in Taiwan. Wufeng, Taichung, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, Executive Yuan. 259. (in Chinese).Google Scholar
Chun, J. C., Ma, S. Y., Kim, S. E., and Lee, H. J. 1997. Physiological responses of Rehmannia glutinosa to paraquat and its tolerance mechanisms. Pestic. Biochem. Physiol. 59:51–63.CrossRefGoogle Scholar
Ekmekci, Y. and Terzioglu, S. 2005. Effects of oxidative stress induced by paraquat on wild and cultivated wheats. Pestic. Biochem. Physiol. 83:69–81.Google Scholar
Foster, J. G. and Hess, J. L. 1980. Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen. Plant Physiol. 66:482–487.Google Scholar
Foyer, C. H., Descourviéres, P., and Kunert, K. J. 1994. Protection against oxygen radicals: an important defence mechanism studied in transgenic plants. Plant Cell Environ. 17:507–523.CrossRefGoogle Scholar
Foyer, C. H., Lopez-Delgado, H., Dat, J. F., and Scott, I. M. 1997. Hydrogen peroxide- and glutathione-associated mechanisms of acclimatory stress tolerance and signalling. Physiol. Plant. 100:241–254.Google Scholar
Fuerst, E. P. and Vaughn, K. C. 1990. Mechanisms of paraquat resistance. Weed Technol. 4:150–156.Google Scholar
Giannopolitis, C. N. and Ries, S. K. 1977. Superoxide dismutase: I. Occurrence in higher plants. Plant Physiol. 59:309–314.Google Scholar
Halliwell, B. 1987. Oxidative damage, lipid peroxidation and antioxidant protection in chloroplasts. Chem. Phys. Lipids. 44:327–340.Google Scholar
Hart, J. J. and Tomaso, J. M. 1994. Sequestration and oxygen radical detoxification as mechanisms of paraquat resistance. Weed Sci. 42:277–284.Google Scholar
Heap, I. M. 2007. International Survey of Herbicide-Resistant Weeds. http://www.weedscience.com. Accessed: April 4, 2007.Google Scholar
Heath, R. L. and Packer, L. 1968. Photoperoxidation in isolated chloroplasts I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125:189–198.Google Scholar
Hidayat, I., Baker, J., and Preston, C. 2006. Pollen-mediated gene flow between paraquat-resistant and susceptible hare barley (Hordeum leporinum). Weed Sci. 54:685–689.Google Scholar
Hung, K. T., Chang, C. J., and Kao, C. H. 2002. Paraquat toxicity is reduced by nitric oxide in rice leaves. J. Plant Physiol. 159:159–166.Google Scholar
Iturbe-Ormaetxe, I., Escuredo, P. R., Arrese-lgor, C., and Becana, M. 1998. Oxidative damage in pea plants exposed to water deficit or paraquat. Plant Physiol. 116:173–181.Google Scholar
Kuk, Y. I., Shin, J. S., Jung, H. I., Guh, J. O., Jung, S., and Burgos, N. R. 2006. Mechanism of paraquat tolerance in cucumber leaves of various ages. Weed Sci. 54:6–15.Google Scholar
Larson, R. A. 1988. The antioxidants of higher plants. Photochem. 27:969–978.Google Scholar
Lascano, H. R., Melchiorre, M. N., Luna, C. M., and Trippi, V. S. 2003. Effect of photooxidative stress induced by paraquat in two wheat cultivars with differential tolerance to water stress. Plant Sci. 164:841–848.Google Scholar
Law, M. Y., Charles, S. A., and Halliwell, B. 1983. Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts: the effect of hydrogen peroxide and of paraquat. Biochem. J. 210:899–903.Google Scholar
Lewinsohn, E. and Gressel, J. 1984. Benzyl viologen-mediated counteraction of diquat and paraquat phytotoxicities. Plant Physiol. 76:125–130.Google Scholar
Mano, J., Ushimaru, T., and Asada, K. 1997. Ascorbate in thylakoid lumen as an endogenous electron donor to photosystem II: Protection of thylakoids from photoinhibition and regeneration of ascorbate in stroma by dehydroascorbate reductase. Photosynth. Res. 53:197–204.CrossRefGoogle Scholar
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7:405–410.Google Scholar
Murgia, I., Tarantino, D., Vannini, C., Bracale, M., Carravier, S., and Soave, C. 2004. Arabidopsis thaliana plants overexpressing thylakoidal ascorbate peroxidase show increased resistance to paraquat-induced photooxidative stress and to nitric oxide-induced cell death. Plant J. 38:940–953.Google Scholar
Nakano, Y. and Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22:867–880.Google Scholar
Noctor, G. and Foyer, C. H. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49:249–279.Google Scholar
Norman, M. A. and Fuerst, E. P. 1997. Interactions of cations with paraquat in leaf sections of resistant and sensitive biotypes of Conyza bonariensis . Pestic. Biochem. Physiol. 57:181–191.Google Scholar
Pallanca, J. E. and Smirnoff, N. 2000. The control of ascorbic acid synthesis and turnover in pea seedlings. J. Exp. Bot. 51:669–674.CrossRefGoogle ScholarPubMed
Polle, A. 2001. Dissecting the superoxide dismutase-ascorbate-glutathione pathway in chloroplasts by metabolic modeling. Computer simulations as a step towards flux analysis. Plant Physiol. 126:445–462.Google Scholar
Pölös, E., Mikulás, J., Szigeti, Z., Matkovics, B., Hai, D. Q., ducz, P., and Lehoczki, E. 1988. Paraquat and atrazine co-resistance in Conyza canadensis (L.) Cronq. Pestic. Biochem. Physiol. 30:142–154.Google Scholar
Powles, S. B. and Cornic, G. 1987. Mechanism of paraquat resistance in Hordeum glaucum. I. Studies with isolated organelles and enzymes. Aust. J. Plant Physiol. 14:81–89.Google Scholar
Pyon, J. Y., Piao, R. Z., Roh, S. W., Shin, S. Y., and Kwak, S. S. 2004. Differential levels of antioxidants in paraquat-resistant and -susceptible Erigeron canadensis biotypes in Korea. Weed Biol. Manag. 4:75–80.Google Scholar
Scandalios, J. G. 1993. Oxygen stress and superoxide dismutases. Plant Physiol. 101:7–12.Google Scholar
Schmmitz-Eiberger, M. and Noga, G. 2001. Reduction of paraquat-induced oxidative stress in Phaseolus vulgaris and Malus domestica leaves by α-tocopherol. Sci. Hortic. 91:153–167.Google Scholar
Shaaltiel, Y. and Gressel, J. 1986. Multienzyme oxygen radical detoxifying system correlated with paraquat resistance in Conyza bonariensis . Pestic. Biochem. Physiol. 26:22–28.Google Scholar
Smith, I. K. 1985. Stimulation of glutathione synthesis in photorespiring plants by catalase inhibitors. Plant Physiol. 79:1044–1047.CrossRefGoogle ScholarPubMed
Srivastava, M. K. and Dwived, U. N. 1998. Salicylic acid modulates glutathione metabolism in pea seedlings. J. Plant Physiol. 153:409–414.Google Scholar
Suntres, Z. E. 2002. Role of antioxidants in paraquat toxicity. Toxicology. 180:65–77.Google Scholar
Szigeti, Z. and Lehoczki, E. 2003. A review of physiological and biochemical aspects of resistance to atrazine and paraquat in Hungarian weeds. Pest Manag. Sci. 59:451–458.Google Scholar
Szigeti, Z., Rácz, I., and Lásztity, D. 2001. Paraquat resistance of weeds- the case of Conyza canadensis (L.) Cronq. Z. Naturforsch. 56c:319–328.Google Scholar
Turcsányi, E., Surányi, G., Lehoczki, E., and Borbély, G. 1994. Superoxide dismutase activity in responses to paraquat resistance in Conyza canadensis (L.) Cronq. J. Plant Physiol. 144:599–606.Google Scholar
Váradi, G., Dark, E., and Lehoczki, E. 2000. Changes in the xanthophyll cycle and fluorescence quenching indicate light-dependent early events in the action of paraquat and the mechanism of resistance to paraquat in Erigeron canadensis (L.) Cronq. Plant Physiol. 123:1459–1469.Google Scholar
Vartak, V. and Bhargava, S. 1999. Photosynthetic performance and antioxidant metabolism in a paraquat-resistant mutant of Chlamydomonas reinhardtii L. Pestic. Biochem. Physiol. 64:9–15.Google Scholar
Vaughn, K. C. 2003. Herbicide resistance work in the United States Department of Agriculture–Agricultural Research Service. Pest Manag. Sci. 59:764–769.CrossRefGoogle ScholarPubMed
Vaughn, K. C. and Fuerst, E. P. 1985. Structural and physiological studies of paraquat-resistant Conyza . Pestic. Biochem. Physiol. 24:86–94.Google Scholar
Ye, B. and Gressel, J. 2000. Transient, oxidant-induced antioxidant transcript and enzyme levels correlate with greater oxidant-resistance in paraquat-resistant Conyza bonariensis . Planta. 211:50–61.Google Scholar
Youngman, R. J. and Dodge, A. D. 1980. Paraquat resistance in Conyza . Plant Physiol. 65:6 (Suppl.): 12.Google Scholar