Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-23T08:26:44.517Z Has data issue: false hasContentIssue false
  • English
  • Français

Resistance to glyphosate in Lolium rigidum. I. Bioevaluation

Published online by Cambridge University Press:  12 June 2017

James Pratley ,
Nigel Urwin ,
Rex Stanton ,
Peter Baines ,
John Broster ,
Kerrie Cullis ,
David Schafer ,
Joseph Bohn  and
Roger Krueger
Nigel Urwin
Affiliation:
Farrer Centre, Charles Sturt University, Wagga Wagga, New South Wales 2678, Australia
Rex Stanton
Affiliation:
Farrer Centre, Charles Sturt University, Wagga Wagga, New South Wales 2678, Australia
Peter Baines
Affiliation:
Farrer Centre, Charles Sturt University, Wagga Wagga, New South Wales 2678, Australia
John Broster
Affiliation:
Farrer Centre, Charles Sturt University, Wagga Wagga, New South Wales 2678, Australia
Kerrie Cullis
Affiliation:
School of Information Studies, Charles Sturt University, Wagga Wigga, New South Wales 2678, Australia
David Schafer
Affiliation:
Monsanto, Life Sciences, St Louis, MO 63167
Joseph Bohn
Affiliation:
Monsanto, Life Sciences, St Louis, MO 63167
Roger Krueger
Affiliation:
Monsanto, Life Sciences, St Louis, MO 63167
Get access Rights & Permissions [Opens in a new window]

Abstract

Glyphosate is the world's most widely used herbicide. It is nonselective and has been used to control a broad range of weed species for the past 20 yr, without the appearance of resistant weed biotypes. However, a biotype of Lolium rigidum from a field in Northern Victoria, Australia, in which glyphosate had been used for the past 15 yr, failed to be controlled by label recommended rates. Based on LD50 values from pot dose-response experiments, this biotype exhibited resistance to glyphosate and was nearly 10-fold more resistant compared to the susceptible biotypes tested. The biotype was resistant to three different salts of glyphosate. The biotype was also nearly threefold more resistant to diclofop-methyl but was susceptible to other commonly used selective and broad-spectrum herbicides. Between the two-leaf and tillering stages of development, a susceptible biotype exhibited a small but significant decrease in tolerance to glyphosate, whereas tolerance of the resistant biotype remained unchanged with age. The resistant phenotype was verified in experiments in which seed was germinated in the presence of glyphosate. Observations on shoot and root growth of seedlings in these experiments suggested that the resistance mechanism might be associated more with the shoot than with the root.

Keywords

Diclofop-methylglyphosateLolium rigidum Gaud. LOLRIGermination inhibitionvisual injury ratingmultiple resistanceresistance phenotypeherbicide toleranceglyphosate ammoniumglyphosate isopropylamineglyphosate trimesiumbiotypeLOLRI
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 47 , Issue 4 , August 1999 , pp. 405 - 411
Copyright
Copyright © 1999 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Atkinson, D. 1985. Toxicological properties of glyphosate—a summary. Pages 127–134 and 210–216 in Grossbord, E. and Atkinson, D., eds. The Herbicide Glyphosate. Toronto: Butterworth.Google Scholar
Boerboom, C. M., Wyse, D. L., and Somers, D. A. 1990. Mechanism of glyphosate tolerance in birdsfoot trefoil (Lotus corniculatus). Weed Sci. 38:463–467.Google Scholar
Bradshaw, L. D., Padgette, S. R., Kimball, S. I., and Wells, B. H. 1997. Perspectives on glyphosate resistance. Weed Technol. 11:189–198.CrossRefGoogle Scholar
DeGennaro, F. P. and Weller, S. C. 1984. Differential sensitivity of field bindweed (Convolvulus arvensis) biotypes to glyphosate. Weed Sci. 32:472–476.CrossRefGoogle Scholar
della-Cioppa, G. and Kishore, G. M. 1988. Import of a precursor protein into chloroplasts is inhibited by the herbicide glyphosate. EMBO (Eur. Mol. Biol. Organ.) J. 7:1299–1305.Google Scholar
Denis, M. and Delrot, S. 1997. Effects of salts and surfactants on foliar uptake and long distance transport of glyphosate. Plant Physiol, Biochem. 35:291–301.Google Scholar
Forlani, G., Nielsen, E., and Racchi, M. L. 1992. A glyphosate-resistant 5-enolpyruvyl-shikimate-3-phosphate synthase confers tolerance to a maize cell line. Plant Sci. 85:9–15.CrossRefGoogle Scholar
Gill, G. 1995. Development of herbicide resistance in annual ryegrass populations (Lolium rigidum Gaud.) in the cropping belt of Western Australia, Aust. J. Exp. Agric. 35:67–72.Google Scholar
Gressel, J. 1996. Fewer constraints than proclaimed to the evolution of glyphosate-resistant weeds. Res. Pestic. Manag. 8:2–5.Google Scholar
Hall, L. M., Holtum, J.A.M., and Powles, S. B. 1994. Mechanisms responsible for cross resistance and multiple resistance. Pages 243–261 in Powles, S. B. and Holtum, J.A.M., eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL: Lewis.Google Scholar
Haughn, G. W. and Somerville, C. R. 1986. Sulfonylurea-resistant mutants of Arabidopsis thaliana . Mol. Gen. Genet. 204:430–434.Google Scholar
Haughn, G. W. and Somerville, C. R. 1990. A mutation causing imidazolinone resistance maps to the Csr1 locus of Arabidopsis thaliana . Plant Physiol. 92:1081–1085.Google Scholar
Heap, J. and Knight, R. 1982. A population of ryegrass tolerant to the herbicide diclofop methyl. J. Aust. Inst. Agric. Sci. 48:156–157.Google Scholar
Heap, J. and Knight, R. 1986. The occurrence of herbicide cross-resistance in a population of annual ryegrass Lolium rigidum, resistant to diclofop methyl. Aust. J. Agric. Res. 37:149–156.Google Scholar
Heap, J. and Knight, R. 1990. Variation in herbicide cross resistance among populations of annual ryegrass (Lolium rigidum) resistant to diclofop methyl. Aust. J. Agric. Res. 41:121–128.Google Scholar
Hollander-Czytko, H., Sommer, I., and Amrhein, N. 1992. Glyphosate tolerance of cultured Corydalis sempervirens cells is acquired by an increased rate of transcription of 5-enolpyruvylshikimate-3-phosphate synthase as well as by a reduced turnover of the enzyme. Plant Mol. Biol. 20:1029–1036.Google Scholar
Kloot, P. M. 1983. The genus Lolium in Australia. Aust. J. Bot. 31:421–435.CrossRefGoogle Scholar
Malik, J., Barry, G., and Kishore, G. 1989. The herbicide glyphosate. Bio-factors 2:17–25.Google Scholar
Matthews, J. M. and Powles, S. B. 1996. Managing herbicide resistant annual ryegrass, southern Australian research. Pages 537–541 in Proceedings of the 11th Australian Weeds Conference. Melbourne: Weed Science Society of Victoria.Google Scholar
Maxwell, B. D. and Mortimer, A. M. 1994. Selection for herbicide resistance. Pages 1–25 in Powles, S. B. and Holtum, J.A.M., eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL: Lewis.Google Scholar
McCullagh, P. and Nelder, J. A. 1989. Generalized Linear Models. London; New York: Chapman and Hall, pp. 98–148.Google Scholar
McGowan, A. A. 1970. Comparative germination patterns of annual grasses in north-eastern Victoria. Aust. J. Agric. Anim. Husb. 10:401–404.CrossRefGoogle Scholar
Murata, M., Ryu, J., Caretto, S., Rao, D., Song, H., and Widholm, J. M. 1998. Stability and culture medium limitations of gene amplification in glyphosate resistant carrot cell lines. J. Plant Physiol. 152:112–117.CrossRefGoogle Scholar
Naylor, B. 1960. Species differentiation in the genus Lolium . Heredity 15:219–233.Google Scholar
Nietschke, B. S., Llewellyn, R. S., Reeves, T. G., Matthews, J. M., and Powles, S. B. 1996. Herbicide resistance in wild oats and annual ryegrass. Page 691 in Proceedings of the 8th Australian Agronomy Conference, Toowoomba. Melbourne: The Australian Society of Agronomy.Google Scholar
Padgette, S. R., Re, D. B., Gasser, C. S., et al. 1991. Site-directed mutagenesis of a conserved region of the 5-enolpyruvylshikimate-3-phosphate synthase active site. J. Biol. Chem. 266:22364–22369.CrossRefGoogle ScholarPubMed
Powles, S., Lorraine-Colwill, D., Dellow, J. D., and Preston, C. 1998. Evolved resistance to glyphosate in L. rigidum (Lolium rigidum) in Australia. Weed Sci. 46:604–607.CrossRefGoogle Scholar
Powles, S. and Matthews, J. 1992. Multiple herbicide resistance in annual ryegrass (Lolium rigidum): a driving force for the adoption of integrated weed management. Pages 75–87 in Denholm, I., Devonshire, A., and Holloman, D., eds. Achievements and Developments in Combating Pest Resistance. London: Elsevier.Google Scholar
Powles, S. B., Preston, C., Bryan, I. B., and Jutsum, A. R. 1997. Herbicide resistance: impact and management. Adv. Agron. 58:57–93.Google Scholar
Pratley, J., Baines, P., Eberbach, P., Incerti, M., and Broster, J. 1996. Glyphosate resistance in annual ryegrass. Page 126 in Virgona, J. and Michalk, D., eds. Proceedings of the 11th Annual Conference of the Grasslands Society of New South Wales. Wagga Wagga, Australia: The Grasslands Society of NSW.Google Scholar
Pratley, J., Ingrey, J., and Graham, R. 1993. Spread of herbicide resistance through seed retail outlets. Pages 151–153 in Proceedings of the 7th Australian Agronomy Conference, Adelaide.Google Scholar
Preston, C., Tardif, F. J., Christopher, J. T., and Powles, S. B. 1996. Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pestic. Biochem. Physiol. 54:123–134.CrossRefGoogle Scholar
Preston, C., Tardif, F. J., Christopher, J. T., and Powles, S. B. 1996. Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pest. Biochem. Physiol. 54:123–134.CrossRefGoogle Scholar
Racchi, M. L., Rebecchi, M., Todesco, G., Neilsen, E., and Forlani, G. 1995. Glyphosate tolerance in maize (Zea mays L.). 2. Selection and characterization of a tolerant somaclone. Euphytica 82:165–173.Google Scholar
Sellin, C., Forlani, G., Dubois, J., Nielsen, E., and Vasseur, J. 1992. Glyphosate tolerance in Cichorium intybus L. var. Magdebourg. Plant Sci. 85:223–231.CrossRefGoogle Scholar
Shyr, Y-Y.J., Hepburn, A. G., and Widholm, J. M. 1992. Glyphosate selected amplification of the 5-enolpyruvylshikimate-3-phosphate synthase gene in cultured carrot cells. Mol. Gen. Genet. 232:377–382.Google Scholar
Steinrucken, H. and Amrhein, N. 1980. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimate-3-phosphate synthase. Biochem. Biophys. Res. Commun. 94:1207–1212.Google Scholar
Tardif, F. J., Holtum, J.A.M., and Powles, S. B. 1993. Occurrence of a herbicide-resistant acetyl coenzyme A carboxylase mutant in annual ryegrass (Lolium rigidum) selected by sethoxydim. Planta 190:176–181.CrossRefGoogle Scholar
Tardif, F. J., Preston, C., Holtum, J.A.M., and Powles, S. B. 1996. Resistance to acetyl-coenzyme A carboxylase-inhibiting herbicides endowed by a single major gene encoding a resistant target site in a biotype of Lolium rigidum . Aust. J. Plant Physiol. 23:15–23.Google Scholar
Verbyla, A. P., Cullis, B. R., Kenwood, M. J., and Welham, S. J. 1999. The analysis of designed experiments using smoothed splines. Appl. Stat. 48:269–312.Google Scholar