Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-05T04:28:43.710Z Has data issue: false hasContentIssue false
  • English
  • Français

Resistance Mechanisms to an Acetolactate Synthase (ALS) Inhibitor in Water Starwort (Myosoton aquaticum) Populations from China

Published online by Cambridge University Press:  20 January 2017

Weitang Liu ,
Cuixia Wu ,
Wenlei Guo ,
Long Du ,
Guohui Yuan  and
Jinxin Wang
Weitang Liu
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai'an 271018, PR China
Cuixia Wu
Affiliation:
Taian Academy of Agricultural Sciences, Tai'an 271000, Shandong, PR China, PR China
Wenlei Guo
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai'an 271018, PR China
Long Du
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai'an 271018, PR China
Guohui Yuan
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai'an 271018, PR China
Jinxin Wang*
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai'an 271018, PR China
*
Corresponding author's E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Overreliance on tribenuron has resulted in resistance evolution in water starwort. This study investigates the resistance mechanisms to tribenuron in water starwort populations from China. The cytochrome P450 monooxygenase (P450) inhibitor malathion increased tribenuron sensitivity in all populations. The decrease in the amount of herbicide dose that causes 50% growth reduction (GR50) for the sensitive (S) population JS24 and the resistant (R) populations JS16 and JS17 were 2.3-, 2.5-, and 4.1-fold, respectively. However, the GR50 values for the R populations were still much higher than those of the S population. This observation indicates that P450-mediated enhanced metabolism is one mechanism for resistance in water starwort. The glutathione-S-transferase (GST) activity could be induced by tribenuron for all tested populations. In particular, the GST activity of JS16 is inherently greater and is more rapidly induced than that of JS17 or JS24. Resistance attributed to mutant acetolactate synthase (ALS) alleles was identified by sequence analysis for each population. Pro197Ser substitution was detected in JS16 and JS17. Molecular markers were also developed to rapidly identify resistance as well as individuals carrying the specific Pro197Ser mutation in water starwort populations. The resistance patterns experiment revealed that the R populations exhibited different levels of resistance to pyrithiobac sodium salt, florasulam, pyroxsulam, and flucarbazone-Na; however, R populations were sensitive to imazethapyr, fluroxypyr-meptyl, 2,4-D butylate, isoproturon, and diflufenican. This study establishes that either one or at least two resistance mechanisms are involved in herbicide resistance in water starwort. Moreover, these mechanisms might contribute to the different levels of resistance to tribenuron among water starwort populations.

Keywords

2,4-D butylatediflufenicanflorasulamflucarbazone-Nafluroxypyr-meptylimazethapyrisoproturonpyrithiobac sodium saltpyroxsulamtribenuronwater starwort, Myosoton aquaticum (L.) Moench.ALSdCAPSherbicide resistanceresistance mechanismsresistance pattern
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 63 , Issue 4 , December 2015 , pp. 770 - 780
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Baerg, RJ, Barrett, M, Polge, ND (1996) Insecticide and insecticide metabolite interactions with cytochrome P450 mediated activities in maize. Pestic Biochem Physiol 55:10–20 Google Scholar
Beckie, HJ, Warwick, SI, Sauder, CA (2012) Basis for herbicide resistance in Canadian populations of wild oat (Avena fatua). Weed Sci 60:10–18 Google Scholar
Bradford, MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254 Google Scholar
Busi, R, Gaines, TA, Vila-Aiub, MM, Powles, SB (2014) Inheritance of evolved resistance to a novel herbicide (pyroxasulfone). Plant Sci 217–218:127–134 Google Scholar
Busi, R, Vila-Aiub, MM, Beckie, HJ, Gaines, TA, Goggin, DE, Kaundun, SS, Lacoste, M, Neve, P, Nissen, SJ, Norsworthy, JK, Renton, M, Shaner, DL, Tranel, PJ, Wright, T, Yu, Q, Powles, SB (2013) Herbicide-resistant weeds: from research and knowledge to future needs. Evol Appl 6:1218–1221 Google Scholar
Christopher, JT, Powles, SB, Holtum, JA (1992) Resistance to acetolactate synthase-inhibiting herbicides in annual ryegrass (Lolium rigidum) involves at least two mechanisms. Plant Physiol 100:1909–1913 Google Scholar
Corbett, CA, Tardif, FJ (2006) Detection of resistance to acetolactate synthase inhibitors in weeds with emphasis on DNA-based techniques: a review. Pest Manag Sci 62:584–597 Google Scholar
Délye, C (2005) Weed resistance to acetyl coenzyme A carboxylase inhibitors: an update. Weed Sci 53:728–746 Google Scholar
Délye, C (2013) Unravelling the genetic bases of non-target-site-based resistance (NTSR) to herbicides: a major challenge for weed science in the forthcoming decade. Pest Manag Sci 69:176–187 Google Scholar
Délye, C, Gardin, J, Boucansaud, K, Chauvel, B, Petit, C (2011a) Non-target-site-based resistance should be the centre of attention for herbicide resistance research: Alopecurus myosuroides as an illustration. Weed Res 51:433–437 Google Scholar
Délye, C, Jasieniuk, M, Le Corre, V (2013) Deciphering the evolution of herbicide resistance in weeds. Trends Genet 29:649–658 Google Scholar
Délye, C, Pernin, F, Scarabel, L (2011b) Evolution and diversity of the mechanisms endowing resistance to herbicides inhibiting acetolactate-synthase (ALS) in corn poppy (Papaver rhoeas L.). Plant Sci 180:333–342 Google Scholar
De Prado, RA, Franco, AR (2004) Cross-resistance and herbicide metabolism in grass weeds in Europe: biochemical and physiological aspects. Weed Sci 52:441–447 Google Scholar
Doyle, JJ, Doyle, JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15 Google Scholar
Edwards, R, Owen, W (1986) Comparison of glutathione-S-transferases of Zea mays responsible for herbicide detoxification in plants and suspension-cultured cells. Planta 169:208–215 Google Scholar
Habig, WH, Pabst, MJ, Jakoby, WB (1974) Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139 Google Scholar
Hamouzova, K, Kosnarova, P, Salava, J, Soukup, J, Hamouz, P (2014) Mechanisms of resistance to acetolactate synthase-inhibiting herbicides in populations of Apera spica-venti from the Czech Republic. Pest Manag Sci 70:541–548 Google Scholar
Hatton, PJ, Dixon, D, Cole, DJ, Edwards, R (1996) Glutathione transferase activities and herbicide selectivity in maize and associated weed species. Pestic Sci 46:267–275 Google Scholar
Irzyk, GP, Fuerst, EP (1993) Purification and characterization of a glutathione-S-transferase from benoxacor-treated maize (Zea mays). Plant Physiol 102:803–810 Google Scholar
Lamoureux, GL, Rusness, DG (1993) Glutathione in the metabolism and detoxification of xenobiotics in plants. Pages 221–237 in Dekok, LJ ed. Sulfur Nutrition and Assimilation in Higher Plants. The Hague, The Netherlands SPB Academic Publishing Google Scholar
Liu, W, Bi, Y, Li, L, Yuan, G, Du, L, Wang, J (2013a) Target-site basis for resistance to acetolactate synthase inhibitor in water chickweed (Myosoton aquaticum L.). Pestic Biochem Physiol 107:50–54 Google Scholar
Liu, W, Bi, Y, Li, L, Yuan, G, Wang, J (2013b) Molecular basis of resistance to tribenuron in water starwort (Myosoton aquaticum) populations from China. Weed Sci 61:390–395 Google Scholar
Liu, W, Yuan, G, Du, L, Guo, W, Li, L, Bi, Y, Wang, J (2015) A novel Pro197Glu substitution in acetolactate synthase (ALS) confers broad-spectrum resistance across ALS inhibitors. Pestic Biochem Physiol 117:31–38 Google Scholar
Ma, R, Kaundun, SS, Tranel, PJ, Riggins, CW, McGinness, DL, Hager, AG, Hawkes, T, McIndoe, E, Riechers, DE (2013) Distinct detoxification mechanisms confer resistance to mesotrione and atrazine in a population of waterhemp. Plant Physiol 163:363–377 Google Scholar
Marshall, R, Hanley, SJ, Hull, R, Moss, SR (2013) The presence of two different target-site resistance mechanisms in individual plants of Alopecurus myosuroides Huds., identified using a quick molecular test for the characterisation of six ALS and seven ACCase SNPs. Pest Manag Sci 69:727–737 Google Scholar
Massa, D, Krenz, B, Gerhards, R (2011) Target-site resistance to ALS-inhibiting herbicides in Apera spica-venti populations is conferred by documented and previously unknown mutations. Weed Res 51:294–303 Google Scholar
Owen, MJ, Goggin, DE, Powles, SB (2012) Non-target-site-based resistance to ALS-inhibiting herbicides in six Bromus rigidus populations from Western Australian cropping fields. Pest Manag Sci 68:1077–1082 Google Scholar
Powles, SB, Yu, Q (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol 61:317–347 Google Scholar
Preston, C (2004) Herbicide resistance in weeds endowed by enhanced detoxification: complications for management. Weed Sci 52:448–453 Google Scholar
Riechers, DE, Irzyk, GP, Jones, SS, Fuerst, EP (1997) Partial characterization of glutathione-S-transferases from wheat (Triticum spp.) and purification of a safener-induced glutathione-S-transferase from Triticum tauschii . Plant Physiol 114:1461–1470 Google Scholar
Riechers, DE, Kreuz, K, Zhang, Q (2010) Detoxification without intoxication: herbicide safeners activate plant defense gene expression. Plant Physiol 153:3–13 Google Scholar
Riechers, DE, Vaughn, KC, Molin, WT (2005) The role of plant glutathione-S-transferases in herbicide metabolism. Pages 216–232 in Clark, JM, Ohkawa, H, eds. Environmental Fate and Safety Management of Agrochemicals. ACS Symposium Series 899. Washington, DC American Chemical Society Google Scholar
Riechers, DE, Zhang, Q, Xu, F, Vaughn, KC (2003) Tissue-specific expression and localization of safener-induced glutathione-S-transferase proteins in Triticum tauschii . Planta 217:831–840 Google Scholar
Roberts, TR, Hutson, DH (1998) Metabolic Pathways of Agrochemicals: Herbicides and Plant Growth Regulators. Cambridge, UK The Royal Society of Chemistry. 573 pGoogle Scholar
Siminszky, B (2006) Plant cytochrome P450-mediated herbicide metabolism. Phytochem Rev 5:445–458 Google Scholar
Tranel, PJ, Wright, TR (2002) Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci 50:700–712 Google Scholar
Tranel, PJ, Wright, TR, Heap, IM (2015) Mutations in Herbicide-Resistant Weeds to ALS Inhibitors. http://www.weedscience.com. Accessed April 27, 2015Google Scholar
Volenberg, DS, Tranel, PJ, Holt, JF, Simmons, FW, Weller, SC, Sharkhuu, A, Riechers, DE (2007) Assessment of two biotypes of Solanum ptycanthum that differ in resistance levels to imazamox. Weed Res 47:353–363 Google Scholar
Yu, Q, Abdallah, I, Han, H, Owen, M, Powles, S (2009) Distinct non-target site mechanisms endow resistance to glyphosate, ACCase and ALS-inhibiting herbicides in multiple herbicide-resistant Lolium rigidum . Planta 230:713–723 Google Scholar
Yu, Q, Han, H, Powles, SB (2008) Mutations of the ALS gene endowing resistance to ALS-inhibiting herbicides in Lolium rigidum populations. Pest Manag Sci 64:1229–1236 Google Scholar
Yu, Q, Powles, SB (2014a) Metabolism-based herbicide resistance and cross-resistance in crop weeds: a threat to herbicide sustainability and global crop production. Plant Physiol 166:1106–1118 Google Scholar
Yu, Q, Powles, SB (2014b) Resistance to AHAS inhibitor herbicides: current understanding. Pest Manag Sci 70:1340–1350 Google Scholar
Zhang, Z (2003) Development of chemical weed control and integrated weed management in China. Weed Biol Manag 3:197–203 Google Scholar