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A Rapid Microtitre Plate Assay for Determining Sensitivity to Photosystem II Herbicides

Published online by Cambridge University Press:  12 June 2017

Michael P. Anderson ,
Curtis N. Bensch  and
Jimmy F. Stritzke
Michael P. Anderson
Affiliation:
Dep. Agron. Oklahoma State Univ., Stillwater, OK 74078
Curtis N. Bensch
Affiliation:
Dep. Agron. Oklahoma State Univ., Stillwater, OK 74078
Jimmy F. Stritzke
Affiliation:
Dep. Agron. Oklahoma State Univ., Stillwater, OK 74078
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Abstract

This paper reports on a microtitre plate version of the 2,6-dichlorophenol-indophenol (DCPIP) reduction assay. DCPIP reduction rates of alfalfa thylakoid membranes were determined by measuring absorbance at 600 nm before and after a 1 min illumination period. The membranes were near light-saturated at intensities above 600 μE m–2 s–1. Saturation of thylakoid membranes with DCPIP occurred above 120 μM. DCPIP reduction rates increased linearly with chlorophyll concentrations from 0.25 to 2 μg. The rate of DCPIP reduction was linear throughout a 2 min illumination period (R2 = 0.99). The assay was sensitive enough to terbacil to differentiate between a 20% change in the I50 concentration. DCPIP reduction rates were sensitive to concentrations of terbacil as low as 100 nM. It only takes 13 minutes to load and read 96 samples using the microtitre plate assay compared to 5.5 h using the conventional procedure.

Keywords

Terbacil, 5-chloro-3-(1,1-dimethylethyl)-6-methyl-2,4-(1H,3H)-pyrimidinedionealfalfa, Medicago sativa L. # MEDSATerbacilatrazineMedicago sativa L.
Type
Physiology, Chemistry, and Biochemostry
Information
Weed Science , Volume 42 , Issue 4 , December 1994 , pp. 517 - 522
Copyright
Copyright © 1994 by the Weed Science Society of America 

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