Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-25T07:08:56.988Z Has data issue: false hasContentIssue false
  • English
  • Français

RAPD analysis of genetic variation among propanil-resistant and -susceptible Echinochloa crus-galli populations in Arkansas

Published online by Cambridge University Press:  20 January 2017

Jeff Rutledge ,
Ronald E. Talbert  and
Clay H. Sneller
Jeff Rutledge
Affiliation:
Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701
Clay H. Sneller
Affiliation:
Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701
Get access Rights & Permissions [Opens in a new window]

Abstract

The extensive use of propanil for weed control in Oryza sativa over the past 30 yr has led to the development of propanil-resistant Echinochloa crus-galli. This problem was first identified in one Arkansas county in 1990. Since that time, 171 resistant populations have been identified in 20 counties. Random amplified polymorphic DNA (RAPD) analysis was used to assess genetic variation among populations of propanil-resistant and -susceptible E. crus-galli in Arkansas and to elucidate the origin of resistance and its dispersal. Sixteen E. crus-galli populations from seven Arkansas counties and one population from Mississippi were analyzed using 13 primers, which amplified a total of 159 repeatable fragments. Genetic distance was calculated among the populations, and the populations were clustered. The analysis revealed two distinct clusters with an average between-cluster genetic distance of 0.436 and average within-cluster distances of 0.012 and 0.029. These clusters may represent different Echinochloa species. Each cluster contained at least one resistant population and populations from different regions of the state. In addition, several resistant populations from different regions of the state were genetically nearly identical. Thus, it is likely that resistant biotypes have spread in Arkansas by seed dispersal and by independent mutation events. This suggests that there are multiple origins of the resistance, and simple control of weed seed dispersal will not stop the spread of resistance.

Keywords

PropanilEchinochloa crus-galli (L.) Beauv. ECHCG, barnyardgrassOryza sativa L., riceGenetic diversitygenetic markersherbicide resistanceweed resistanceECHCG
Type
Research Article
Information
Weed Science , Volume 48 , Issue 6 , December 2000 , pp. 669 - 674
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

LITERATURE CITED

Baltazar, A. M. and Smith, R. J. 1994. Propanil-resistant barnyardgrass (Echinochloa crus-galli) control in rice (Oryza sativa) . Weed Technol. 8:576–581.Google Scholar
Barrett, S.C.H. 1988. Genetics and evolution of agricultural weeds. Pages 57–75 In Altieri, M. A. and Liebman, M., eds. Weed Management in Agroecosystems: Ecological Approaches. Boca Raton, FL: CRC Press.Google Scholar
Carey, V. F. III, Hoagland, R. E., and Talbert, R. E. 1995. Verification and distribution of propanil-resistant barnyardgrass (Echinochloa crus-galli) in Arkansas. Weed Technol. 9:366–372.Google Scholar
Hilu, K. W. 1994. Evidence from RAPD markers in the evolution of Echinochloa millets (Poaceae). Plant Syst. Evol. 189:247–257.CrossRefGoogle Scholar
Holm, L. G., Pluncknett, D. L., Pancho, J. V., and Herberger, J. P. 1977. The World's Worst Weeds. Honolulu: University Press of Hawaii, pp. 32–40.Google Scholar
Katzir, N., Portnoy, V., Tzuri, G., Castejon-Munoz, M., and Joel, D. M. 1996. Use of random amplified polymorphic DNA (RAPD) markers in the study of the parasitic weed Orobanche . Theor. Appl. Genet. 93:367–372.Google Scholar
Keim, P., Olson, T. C., and Shoemaker, R. C. 1988. A rapid protocol for isolating soybean DNA. Soybean Genet. Newsl. 15:150–152.Google Scholar
Lopez-Martinez, N., De Prado, R., Finch, R. P., and Marshall, G. 1995. A molecular assessment of genetic diversity in Echinochloa spp. Pages 445–450 In Proceedings of the Brighton Crop Protection Conf.— Weeds—1995. Volume 1. Farnham, Great Britain: British Crop Protection Council.Google Scholar
Lopez-Martinez, N., Pujadas Salva, A., Finch, R. P., and DePrado, R. 1999. Molecular markers indicate intraspecific variation in the control of Echinochloa spp. with quinclorac. Weed Sci. 47:310–315.Google Scholar
Moodie, M., Finch, R. P., and Marshall, G. 1997. Analysis of genetic variation in wild mustard (Sinapis arvensis) using molecular markers. Weed Sci. 45:102–107.Google Scholar
Neuffer, B. 1996. RAPD analysis in colonial and ancestral populations of Capsella bursa-pastoris (L.) Med. (Brassicaceae). Biochem. Syst. Ecol. 24:393–403.Google Scholar
Nissen, S. J., Masters, R. A., Lee, D. J., and Rowe, M. L. 1995. DNA-based marker systems to determine genetic diversity of weedy species and their application to biocontrol. Weed Sci. 43:504–513.Google Scholar
Paran, I., Gidoni, D., and Jacobsohn, R. 1997. Variation between and within broomrape (Orobanche) species revealed by RAPD markers. Heredity 78:68–74.Google Scholar
Rowe, M. L., Lee, D. J., Nissen, S. J., Bowditch, B. M., and Masters, R. A. 1997. Genetic variation in North American leafy spurge (Euphorbia esula) determined by DNA markers. Weed Sci. 45:446–454.Google Scholar
Smith, R. J. Jr. 1961. 3,4-Dichloropropionanilide for control of barnyardgrass in rice. Weeds 9:318–322.Google Scholar
Smith, R. J. Jr. 1965. Propanil and mixtures with propanil for weed control in rice. Weeds 13:236–238.CrossRefGoogle Scholar
Smith, R. J. Jr. 1974. Competition of barnyardgrass with rice cultivars. Weed Sci. 22:423–426.Google Scholar
Smith, R. J. Jr. 1988. Weed thresholds in southern U.S. rice, Oryza sativa . Weed Technol. 2:232–241.CrossRefGoogle Scholar
Smith, R. J. Jr. and Khodayari, K. 1985. Herbicide treatments for control of weeds in dry-seeded rice (Oryza sativa). Weed Sci. 33:686–692.Google Scholar
Sneller, C. H., Miles, J. W., and Hoyt, J. M. 1997. Agronomic performance of soybean plant introductions and their genetic similarity to elite lines. Crop Sci. 37:1595–1600.Google Scholar
Sokal, R. and Rohlf, J. F. 1995. Biometry. 3rd ed. New York: W. H. Freeman, pp. New York7.Google Scholar
Sweeney, P. M. and Danneberger, T. K. 1995. RAPD characterization of Poa annua L. populations in golf course greens and fairways. Crop Sci. 35:1676–1680.Google Scholar
Talbert, R. E., Carey, V. F. III, Kitt, M. J., Helms, R. S., and Black, H. L. 1995. Control, biology and ecology of propanil-resistant barnyardgrass. Pages 23–31 In Wells, B. R., ed. Arkansas Rice Experiment Studies 1994. Fayetteville, AR: Arkansas Agricultural Experiment Station Research Ser. 446.Google Scholar
Talbert, R. E., Norsworthy, J. K., Schmidt, L. A., Baines, C. B., Daou, H., and Baldwin, F. L. 1997. Characterization and control of propanil-resistant barnyardgrass. Pages 44–52 In Norman, R. J. and Johnston, T. H., eds. Arkansas Rice Experiment Studies 1996. Fayetteville, AR: Arkansas Agricultural Experiment Station Research Ser. 456.Google Scholar
Talbert, R. E., Schmidt, L. A., Norsworthy, J. K., Rutledge, J. S., and Fox, W. L. 1998. Confirmation of propanil-resistant barnyardgrass and strategies for control. Pages 80–90 In Norman, R. J. and Johnston, T. H., eds. Arkansas Rice Experiment Studies 1997. Fayetteville, AR: Arkansas Agricultural Experiment Station Research Ser. 460.Google Scholar
Vellekoop, P., Buntjer, J. B., Maas, J. W., and van Brederode, J. 1996. Can the spread of agriculture in Europe be followed by tracing the spread of the weed Silene latifolia . A RAPD study. Theor. Appl. Genet. 92:1085–1090.Google Scholar
Welsh, J. and McClelland, M. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18:7213–7218.Google Scholar
Williams, J.G.K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., and Tingey, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531–6535.Google Scholar