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Phytotoxicity of 2,4-D and 2,4-Dichlorophenol to Red Clover (Trifolium pratense)

Published online by Cambridge University Press:  12 June 2017

S. G. Taylor ,
D. G. Shilling ,
K. H. Quesenberry  and
G. R. Chaudhry
S. G. Taylor
Affiliation:
Agron. Dep., Univ. Florida, Gainesville, FL 32611
D. G. Shilling
Affiliation:
Agron. Dep., Univ. Florida, Gainesville, FL 32611
K. H. Quesenberry
Affiliation:
Agron. Dep., Univ. Florida, Gainesville, FL 32611
G. R. Chaudhry
Affiliation:
Soil Sci. Dep., Univ. Florida, Gainesville, FL 32611
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Abstract

Whole plant and tissue culture experiments were conducted to determine the difference in phytotoxicity of 2,4-D and its metabolite, 2,4-DCP, to red clover. At the whole plant level, the mean concentration of 2,4-DCP (10 mM) required to cause 50% growth inhibition (I50) of shoot dry weight was 24 times greater than for 2,4-D (0.42 mM). Using callus tissue, the I50 value for 2,4-DCP (0.28 mM) was 22 times greater than for 2,4-D (0.013 mM) based on dry weights. The callus tissue was 36 and 32 times more sensitive to 2,4-DCP and 2,4-D than shoot tissue based on dry weights, respectively. These data indicate that 2,4-DCP was less phytotoxic than 2,4-D to red clover both in vitro and in vivo.

Keywords

2,4-D (2,4-dichlorophenoxy)acetic acid2,4-DCP, 2,4-dichlorophenolred clover, Trifolium pratense L.Herbicide toleranceherbicide metabolitetissue culturecallus
Type
Special Topics
Information
Weed Science , Volume 37 , Issue 6 , November 1989 , pp. 825 - 829
Copyright
Copyright © 1989 by the Weed Science Society of America 

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References

Literature Cited

1. Ashton, F. M. and Crafts, A. S. 1981. Phenoxys. Pages 272–302 in Mode of Action of Herbicides. John Wiley and Sons, New York.Google Scholar
2. Burton, J. D. and Balke, N. E. 1988. Glyphosate uptake by suspension-cultured potato (Solanum tuberosum) and S. brevidens cells. Weed Sci. 36:146–153.Google Scholar
3. Chaleff, R. S. and Parsons, M. F. 1978. Direct selection in vitro for herbicide-resistant mutants of Nicotiana tabacum . Proc. Nat. Acad. Sci. U.S.A. 75:5104–5107.Google Scholar
4. Chaudhry, G. R. and Huang, G. H. 1988. Isolation and characterization of a new plasmid from a Flavobacterium sp. which carries the gene for degradation of 2,4-dichlorophenoxy acetate. J. Bacteriol. 170:3897–3902.CrossRefGoogle Scholar
5. Comai, L., Sen, L., and Stalker, D. 1983. An altered aroA gene product confers resistance to the herbicide glyphosate. Science 221:370–371.Google Scholar
6. Comai, L., Facciotti, D., Hiatt, W., Thompson, G., Rose, R., and Stalker, D. 1985. Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317:741–744.Google Scholar
7. Devine, T. E., Seaney, R. R., Linseoll, D. L., Hagin, R. D., and Brace, N. 1975. Results of breeding for tolerance to 2,4-D in birdsfoot trefoil. Crop Sci. 15:721–724.Google Scholar
8. Eisinger, W. R. and Moore, D. J. 1971. Growth-regulating properties of picloram, 4-amino-3,5,6-trichloropicolinic acid. Can. J. Bot. 49:889–897.Google Scholar
9. Faulkner, J. S. 1975. A paraquat tolerant line in Lolium perenne . Pages 349–359 in Proc. 1st European Weed Res. Soc. Symp. Paris Biol. Cont. Grassweeds Eur., Paris, France.Google Scholar
10. Faulkner, J. S. 1982. Breeding herbicide-tolerant crop cultivars by conventional methods. Pages 235–256 in Herbicide Resistance in Plants, LeBaron, H. M. and Gressel, J., eds. John Wiley and Sons, New York.Google Scholar
11. Fraley, R. T., Rogers, S. G., and Horsch, R. B. 1986. Genetic transformation in higher plants. CRC Critical Rev. in Plant Sci. 4(1):1–46.Google Scholar
12. Henderson, J. D. and Claydon, R. B. 1983. Screening red clover for tolerance to 2,4-D. Proc. New Zealand Weed Pest Control Conf. 36:21–24.CrossRefGoogle Scholar
13. Jensen, K.I.N. 1982. The role of uptake, translocation and metabolism in the differential intraspecific responses to herbicides. Pages 133–162 in Herbicide Resistance in Plants, LeBaron, H. M. and Gressel, J., eds. John Wiley and Sons, New York.Google Scholar
14. Meredith, C. P. and Carlson, P. S. 1982. Herbicide resistance in plant cell cultures. Pages 275–291 in Herbicide Resistance in Plants, LeBaron, H. M. and Gressel, J., eds. John Wiley and Sons, New York.Google Scholar
15. Moreland, D. E. 1980. Mechanisms of action of herbicides. Ann. Rev. Plant Physiol. 31:597–638.Google Scholar
16. Phillips, G. C. and Collins, G. B. 1979. In vitro tissue culture of selected legumes and plant regeneration from callus cultures of red clover. Crop Sci. 19:59–64.CrossRefGoogle Scholar
17. Phillips, G. C. and Collins, G. B. 1980. Somatic embryogenesis from cell suspension cultures of red clover. Crop Sci. 20:323–326.Google Scholar
18. Shah, D. M., Horsch, R. B., Klee, H. J., Kishore, G. M., Winter, J. A., Tumer, N. E., Hironaka, C. M., Sanders, P. R., Gasser, C. S., Aykent, S., Siegel, N. R., Rogers, S. G., and Fraley, R. T. 1986. Engineering herbicide tolerance in transgenic plants. Science 233:478–481.Google Scholar
19. Stalker, D., Hiatt, W., and Comai, L. 1985. A single amino acid substitution in the enzyme enolpyruvylshikimate-3-phosphate synthase confers resistance to the herbicide glyphosate. J. Biol. Chem. 260: 4724–4728.Google Scholar
20. Swanson, E. B. and Tomes, D. T. 1979. Plant regeneration from callus cultures of Lotus corniculatus and the selection and characterization of 2,4-D tolerant cell lines. Can. J. Bot. 58:1205–1209.Google Scholar
21. Swanson, E. B. and Tomes, D. T. 1983. Evaluation of birdsfoot trefoil regenerated plants and their progeny after in vitro selection for 2,4-dichlorophenoxy acetic acid tolerance. Plant Sci. Lett. 29:19–24.Google Scholar
22. Taylor, R. W., Griffin, J. L., and Meche, G. A. 1982. Evaluation of annual and perennial clovers for tolerance to 2,4-D. Lotus Newsl. 14:13–14.Google Scholar
23. Taylor, S. G. 1987. In vitro and in vivo methods of selecting for tolerance to the herbicide 2,4-D in red clover (Trifolium pratense L.). Ph.D. Thesis, Univ. Florida, Gainesville. 82 pp.Google Scholar
24. Wright, C. E. and Faulkner, J. S. 1982. Effective selection for tolerance to grass-killing herbicides in perennial ryegrass (Lolium perenne L.). Pages 210–212 in Proc. XIV Int. Grassland Congress, Smith, J. A. and Hayes, V. W., eds. Lexington, KY Westview Press, Boulder, CO.Google Scholar
25. Zilkah, S. and Gressel, J. 1977. Cell cultures vs. whole plants for measuring phytotoxicity. Ill. Correlations between phytotoxicities in cell suspension cultures, calli and seedlings. Plant Cell Physiol. 18: 815–820.Google Scholar