Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-24T22:47:59.956Z Has data issue: false hasContentIssue false
  • English
  • Français

A Multistate Study of the Association Between Glyphosate Resistance and EPSPS Gene Amplification in Waterhemp (Amaranthus tuberculatus)

Published online by Cambridge University Press:  20 January 2017

Laura A. Chatham ,
Kevin W. Bradley ,
Greg R. Kruger ,
James R. Martin ,
Micheal D. K. Owen ,
Dallas E. Peterson ,
Jugulam Mithila  and
Patrick J. Tranel
Laura A. Chatham
Affiliation:
Department of Crop Science, University of Illinois, Urbana, IL 61801
Kevin W. Bradley
Affiliation:
Division of Plant Sciences, University of Missouri, Columbia, MO 65211
Greg R. Kruger
Affiliation:
West Central Research and Extension Center, University of Nebraska-Lincoln, North Platte, NE 69101
James R. Martin
Affiliation:
Department of Plant and Soil Sciences, Research and Education Center, Princeton, KY
Micheal D. K. Owen
Affiliation:
Agronomy Department, Iowa State University, Ames IA 50011
Dallas E. Peterson
Affiliation:
Department of Agronomy, Kansas State University, Manhattan KS 66506
Jugulam Mithila
Affiliation:
Department of Agronomy, Kansas State University, Manhattan KS 66506
Patrick J. Tranel*
Affiliation:
Department of Crop Science, University of Illinois, Urbana, IL 61801
*
Corresponding author's E-mail: [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Waterhemp is an increasingly problematic weed in the U.S. Midwest, having now evolved resistances to herbicides from six different site-of-action groups. Glyphosate-resistant waterhemp in the Midwest is especially concerning given the economic importance of glyphosate in corn and soybean production. Amplification of the target-site gene, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was found to be the mechanism of glyphosate resistance in Palmer amaranth, a species closely related to waterhemp. Here, the relationship between glyphosate resistance and EPSPS gene amplification in waterhemp was investigated. Glyphosate dose response studies were performed at field sites with glyphosate-resistant waterhemp in Illinois, Kansas, Kentucky, Missouri, and Nebraska, and relative EPSPS copy number of survivors was determined via quantitative real-time polymerase chain reaction (qPCR). Waterhemp control increased with increasing glyphosate rate at all locations, but no population was completely controlled even at the highest rate (3,360 g ae ha−1). EPSPS gene amplification was present in plants from four of five locations (Illinois, Kansas, Missouri, and Nebraska) and the proportion of plants with elevated copy number was generally higher in survivors from glyphosate-treated plots than in plants from the untreated control plots. Copy number magnitude varied by site, but an overall trend of increasing copy number with increasing rate was observed in populations with gene amplification, suggesting that waterhemp plants with more EPSPS copies are more resistant. Survivors from the Kentucky population did not have elevated EPSPS copy number. Instead, resistance in this population was attributed to the EPSPS Pro106Ser mutation. Results herein show a quantitative relationship between glyphosate resistance and EPSPS gene amplification in some waterhemp populations, while highlighting that other mechanisms also confer glyphosate resistance in waterhemp.

Keywords

Glyphosatecommon waterhemp, Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea and TardifPalmer amaranth, Amaranthus palmeri S. Wats AMAPAcorn, Zea mays L.soybean, Glycine max (L.) Merr.Dose responseEPSPS gene amplificationglyphosate-resistant waterhempPro106Ser mutation
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 63 , Issue 3 , September 2015 , pp. 569 - 577
Copyright
Copyright © Weed Science Society of America 

References

Literature Cited

Baerson, SR, Rodriguez, DJ, Tran, M, Feng, Y, Viest, NA, Dill, GM (2002) Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol. 129:1265–1275 Google Scholar
Bell, MS, Hager, AG, Tranel, PJ (2013) Multiple resistance to herbicides from four site-of-action groups in waterhemp (Amaranthus tuberculatus). Weed Sci. 61:460–468 Google Scholar
Bell, MS, Tranel, PJ, Riggins, CW (2009) Glyphosate resistance in waterhemp: inheritance and EPSPS copy number. North Central Weed Science Society Abstract 64. [CD-ROM Computer File]. Las Cruces, NM North Central Weed Science Society Google Scholar
Chatham, LA, Riggins, C, Owen, MD, Tranel, P (2012) Association of EPSPS gene amplification with glyphosate resistance in waterhemp. Pages Page 41 in Proceedings of the North Central Weed Science Society. Las Cruces, NM North Central Weed Science Society Google Scholar
Chatham, LA, Wu, C, Riggins, CW, Hager, AG, Young, BG, Roskamp, GK, Tranel, PJ (2015) EPSPS gene amplification is present in the majority of glyphosate-resistant Illinois waterhemp (Amaranthus tuberculatus) populations. Weed Technol. 29:48–55 Google Scholar
Délye, C, Duhoux, A, Pernin, F, Riggins, CW, Tranel, PJ (2015) Molecular mechanisms of herbicide resistance. Weed Sci. 63: 91–115 Google Scholar
Doyle, JJ, Doyle, JL (1990) Isolation of plant DNA from fresh tissue. Focus. 12:13–15 Google Scholar
Duke, SO, Powles, SB (2008) Glyphosate: a once-in-a-century herbicide. Pest Manag Sci. 64:319–325 Google Scholar
Gaines, TA, Wright, AA, Molin, WT, Lorentz, L, Riggins, CW, Tranel, PJ, Beffa, R, Westra, P, Powles, SB (2013) Identification of genetic elements associated with EPSPS gene amplification. PloS one. 8:e65819 Google Scholar
Gaines, TA, Zhang, W, Wang, D, Bukun, B, Chisholm, ST, Shaner, DL, Nissen, SJ, Patzoldt, WL, Tranel, PJ, Culpepper, AS, Grey, TL, Webster, TM, Vencill, WK, Sammons, RD, Jiang, J, Preston, C, Leach, JE, Westra, P (2010) Gene amplification confers glyphosate resistance in Amaranthus palmeri . Proc Natl Acad Sci U S A. 107:1029–1034 Google Scholar
Ge, X, d'Avignon, DA, Ackerman, JJH, Sammons, RD (2010) Rapid vacuolar sequestration: the horseweed glyphosate resistance mechanism. Pest Manag Sci. 66:345–348 Google Scholar
Hager, AG, Wax, LM, Stoller, EW, Bollero, GA (2002) Common waterhemp (Amaranthus rudis) interference in soybean. Weed Sci. 50:607–610 Google Scholar
Heap, IM (2014) International Survey of Herbicide Resistant Weeds. www.weedscience.org. Accessed October 3, 2014Google Scholar
Legleiter, TR, Bradley, KW (2008) Glyphosate and multiple herbicide resistance in common waterhemp (Amaranthus rudis) populations from Missouri. Weed Sci. 56:582–587 Google Scholar
Lorraine-Colwill, DF, Powles, SB, Hawkes, TR, Hollinshead, PH, Warner, SAJ, Preston, C (2003) Investigations into the mechanism of glyphosate resistance in Lolium rigidum . Pestic Biochem Physiol. 74:62–72 Google Scholar
Ma, R, Kaundun, SS, Tranel, PJ, Riggins, CW, McGinness, DL, Hager, AG, Hawkes, T, McIndoes, E, Riechers, DE (2013) Distinct detoxification mechanisms confer resistance to mesotrione and atrazine in a population of waterhemp. Plant Physiol. 163:363–377 Google Scholar
Nandula, VK, Ray, JD, Ribeiro, DN, Pand, Z, Reddy, KN (2013) Glyphosate resistance in tall waterhemp (Amaranthus tuberculatus) from Mississippi is due to both altered target-site and nontarget-site mechanisms. Weed Sci. 61:374–383 Google Scholar
Patton, BP, Witt, W, Martin, JR (2012) Multiple resistance issues within Kentucky waterhemp populations. in Proceedings of the Weed Science Society of America. Lawrence, KS: Weed Science Society of America. http://wssaabstracts.com/public/9/abstract-176.html. Accessed June 25, 2014 [Abstract 176]Google Scholar
Powles, SB (2010) Gene amplification delivers glyphosate-resistant weed evolution. Proc Natl Acad Sci U S A. 107:955–956 Google Scholar
R Development Core Team. 2014. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. http://www.R-project.org. Accessed March 26, 2014Google Scholar
Ribeiro, DN, Pan, Z, Duke, SO, Nandula, VK, Baldwin, BS, Shaw, DR, Dayan, FE (2014) Involvement of facultative apomixes in inheritance of EPSPS gene amplification in glyphosate-resistant Amaranthus palmeri . Planta. 239:199–212 Google Scholar
Salas, RA, Dayan, FE, Pan, Z, Watson, SB, Dickson, JW, Scott, RC, Burgos, NR (2012) EPSPS gene amplification in glyphosate-resistant Italian Ryegrass (Lolium perenne ssp. multiflorum) from Arkansas, USA. Pest Manag Sci. 68:1223–1230 Google Scholar
Schultz, JL, Chatham, LA, Riggins, CW, Tranel, PJ, Bradley, KW (2015) Distribution of herbicide resistances and molecular mechanisms conferring resistance in Missouri waterhemp (Amaranthus rudis Sauer) populations. Weed Sci. 63:336–345 Google Scholar
Shaner, DL, Lindenmeyer, RB, Ostlie, MH (2011) What have the mechanisms of resistance to glyphosate taught us? Pest Manag Sci. 68:3–9 Google Scholar
Steckel, LE, Sprague, CL (2004) Common waterhemp (Amaranthus rudis) interference in corn. Weed Sci. 52:359–364 Google Scholar
Steinrücken, HC, Amrbein, N (1980) The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase. Biochem Biophys Res Commun. 94:1207–1212 Google Scholar
Tamura, K, Stecher, G, Peterson, D, Filipski, A, Kumar, S (2013) MEGA6: molecular evolutionary genetics analysis. Version 6.0. Mol Biol Evol. 30:2725–2729 Google Scholar
Tranel, PJ, Riggins, CW, Bell, MS, Hager, AG (2010) Herbicide resistances in Amaranthus tuberculatus: a call for new options. J Agric Food Chem. 59:5808–5812 Google Scholar
Tranel, PJ, Trucco, F (2009) 21st-century weed science: a call for Amaranthus genomics. Pp 53–81 in Stewart CN Jr, ed. Weedy and Invasive Plant Genomics. Ames, IA Blackwell Google Scholar
Wait, JD, Johnson, WG, Massey, RE (1999) Weed management with reduced rates of glyphosate in no-till, narrow-row, glyphosate-resistant soybean (Glycine max). Weed Technol. 13:478–483 Google Scholar
Wakelin, AM, Preston, C (2006) A target-site mutation is present in a glyphosate-resistant Lolium rigidum population. Weed Res. 46:432–440 Google Scholar
Wiersma, AT, Chisholm, ST, Godar, AS, Stahlman, PW, Leach, J, Westra, P (2012) Gene amplification of EPSP synthase in glyphosate resistant Kochia scoparia . Pages Page 97 in Proceedings of the Western Society of Weed Science. Las Cruces, NM Western Society of Weed Science Google Scholar
Young, BG, Young, JM, Gonzini, LC, Hart, SE, Wax, LE, Kapusta, G (2001) Weed management in narrow- and wide-row glyphosate-resistant soybean (Glycine max). Weed Technol. 15:112–121 Google Scholar