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Morphology, Development, and Recrystallization of Epicuticular Waxes of Johnsongrass (Sorghum halepense)

Published online by Cambridge University Press:  12 June 2017

Chester G. McWhorter ,
Rex N. Paul  and
William L. Barrentine
Chester G. McWhorter
Affiliation:
Plant Physiol. and Biol., South. Weed Sci. Lab., U.S. Dep. Agric., Agric. Res. Serv., and Plant Physiol. Delta Branch Mississippi Agric. and For. Exp. Stn., respectively, Stoneville, MS 38776
Rex N. Paul
Affiliation:
Plant Physiol. and Biol., South. Weed Sci. Lab., U.S. Dep. Agric., Agric. Res. Serv., and Plant Physiol. Delta Branch Mississippi Agric. and For. Exp. Stn., respectively, Stoneville, MS 38776
William L. Barrentine
Affiliation:
Plant Physiol. and Biol., South. Weed Sci. Lab., U.S. Dep. Agric., Agric. Res. Serv., and Plant Physiol. Delta Branch Mississippi Agric. and For. Exp. Stn., respectively, Stoneville, MS 38776
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Abstract

Johnsongrass leaves were covered with epicuticular wax that varied from 16 to 25 μg/cm2 on leaf blades and 56 to 206 μg/cm2 on leaf sheaths. At emergence, leaves were covered with a layer of smooth amorphous wax, but crystalline wax (wax plates) began to form on the amorphous wax within 1 or 2 days. This continued until all leaf surfaces were covered with wax plates. At 3 to 4 weeks of age, a smooth layer of coalescence wax was deposited over the wax plates. Formation of coalescence wax continued until nearly all leaf surfaces were covered with a smooth wax layer. Production of wax filaments began when plants were 3 to 4 weeks old and these tubular structures extended 100 to 200 μm above all other wax formations. Deposition of amorphous wax continued after stomata closed in the darkness, sealing over stomata, but the wax layer was broken when stomata opened again in the light. A capillary method was devised that was used to evaporate chloroform containing leaf waxes through 0.1- to 1.2-μm pores in inert filters to recrystallize amorphous wax and wax plates similar to that produced on johnsongrass leaves. Recrystallization of wax from wax filaments dissolved in chloroform produced the same structures of amorphous wax and wax plates as when only wax from leaves with amorphous wax and wax plates was used. Wax washed from leaves also produced wax plates and a variety of crystalline structures on the walls of glass vials after chloroform solutions were evaporated. This result indicated that the morphology of epicuticular waxes is influenced more by their inherent chemical and physical properties than by underlying cells or the cuticular membrane.

Keywords

Johnsongrass, Sorghum halepense (L.) Pers. ♯3 SORHAWax crystalswax plateswax morphologywax filamentswax bloomelectron microscopySORHA
Type
Weed Biology and Ecology
Information
Weed Science , Volume 38 , Issue 1 , January 1990 , pp. 22 - 33
Copyright
Copyright © 1990 by the Weed Science Society of America 

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References

Literature Cited

1. Akhavein, A. A. and Linscott, D. L. 1968. The dipyridylium herbicides, paraquat and diquat. Residue Rev. 23:97–145.Google ScholarPubMed
2. Amelunxen, F., Morgenroth, K., and Picksak, T. 1967. Untersuchungen an der epidermis mit dem Stereoscan-elektronenmikroskop. Z. Pflanzenphysiol. 57:79–95.Google Scholar
3. Atkin, D.S.J. and Hamilton, R. J. 1982. Surface of Sorghum bicolor . Pages 231–236 in Cutler, D. F., Alvin, K. L., and Price, C. E., eds. The Plant Cuticle. Academic Press, London.Google Scholar
4. Baker, E. A. 1982. Chemistry and morphology of plant epicuticular waxes. Pages 139–166 in Cutler, D. F., Alvin, K. L., and Price, C. E., eds. The Plant Cuticle. Academic Press, London.Google Scholar
5. Baker, E. A. and Bukovac, M. J. 1971. Characterization of the components of plant cuticles in relation to the penetration of 2,4-D. Ann. Appl. Biol. 67:243–253.Google Scholar
6. Baker, E. A., Bukovac, M. J., and Hunt, G. M. 1982. Composition of tomato fruit cuticle as related to fruit growth and development. Pages 33–44 in Cutler, D. F., Alvin, K. L., and Price, C. E., eds. The Plant Cuticle. Academic Press, London.Google Scholar
7. Baker, E. A. and Martin, J. T. 1967. Studies on plant cuticle. X. The cuticles of plants of related families. Ann. Appl. Biol. 60:313–319.CrossRefGoogle Scholar
8. Baker, E. A. and Procopiou, J. 1980. Effect of soil moisture status on leaf surface wax yields of some drought resistant species. J. Hortic. Sci. 55:85–87.Google Scholar
9. Bikales, N. M. 1972. Waxes. Pages 768–779 in Mark, H. F. and Gaylord, N. G., eds. Encyclopedia of Polymer Science and Technology. Vol. 14. Interscience Publ., New York.Google Scholar
10. Bystrom, B. G., Glater, R. B., Scott, F. M., and Bowler, E.S.C. 1968. Leaf surface of Beta vulgaris-Electron microscope study. Bot. Gaz. 129:133–138.Google Scholar
11. Darlington, W. A. and Barry, J. B. 1965. Effects of chloroform and surfactants on permeability of apricot leaf cuticle. J. Agric. Food Chem. 13:76–78.Google Scholar
12. Freeman, B., Albrigo, L. G., and Biggs, J. H. 1979. Cuticular waxes of developing leaves and fruit of blueberry, Vaccinium ashei Reade c v. Bluegem. J. Hortic. Sci. 104:398–403.Google Scholar
13. Hall, D. M. 1967. Wax microchannels in the epidermis of white clover. Science 158:505–506.Google Scholar
14. Hall, D. M. 1967. The ultrastructure of wax deposits on plant leaf surfaces. II. Cuticular pores and wax formation. J. Ultrastruct. Res. 17: 34–44.Google Scholar
15. Hall, D. M. and Donaldson, L. A. 1962. Secretion from pores of surface wax on plant leaves. Nature, London 194:1196.CrossRefGoogle Scholar
16. Hall, D. M. and Donaldson, L. A. 1963. The ultrastructure of wax deposits on plant leaf surfaces. I. Growth of wax on leaves of Trifolium repens . J. Ultrastruct. Res. 9:259–267.Google Scholar
17. Hallam, N. D. 1964. Sectioning and electron microscopy of Eucalypt leaf waxes. Aust. J. Biol. Sci. 17:587–590.Google Scholar
18. Hallam, N. D. 1970. Growth and regeneration of waxes on the leaves of Eucalyptus . Planta 93:257–268.CrossRefGoogle ScholarPubMed
19. Hallam, N. D. 1970. Leaf wax fine structure and ontogeny in Eucalyptus demonstrated by means of a specialized fixation technique. J. Microscopy 92:137–144.Google Scholar
20. Hallam, N. D. 1982. Fine structure of the leaf cuticle and the origin of leaf waxes. Pages 197–214 in Cutler, D. F., Alvin, K. L., and Price, C. E., eds. The Plant Cuticle. Academic Press, London.Google Scholar
21. Hallman, N. D. and Juniper, B. E. 1971. The anatomy of the leaf surface. Pages 3–37 in Preece, T. F. and Dickinson, C. H., eds. Ecology of Leaf Surface Micro-organisms. Academic Press, London.Google Scholar
22. Hamilton, R. J., McCann, A. W., Sewell, P. A., and Merrall, G. T. 1982. Foliar uptake of the wild oat herbicide flamprop-methyl by wheat. Pages 303–313 in Cutler, D. F., Alvin, K. L., and Price, C. E., eds. The Plant Cuticle. Academic Press, London.Google Scholar
23. Holloway, P. J. and Baker, E. A. 1970. The cuticles of some Angiosperm leaves and fruits. Ann. Appl. Biol. 66:145–154.Google Scholar
24. Hull, H. M., Davis, D. G., and Stolzenberg, G. E. 1982. Action of adjuvants on plant surfaces. Pages 26–67 in Hodgson, R. H., ed. Adjuvants for Herbicides. Weed Sci. Soc. Am., Champaign, IL.Google Scholar
25. Jarvis, L. R. and Waldrop, A. B. 1974. The development of the cuticle in Phormium tenax . Planta 119:101–112.Google Scholar
26. Jeffree, C. E. 1974. Method for recrystallizing selected components of plant epicuticular waxes as surfaces for the growth of micro-organisms. Trans. Br. Mycol. Soc. 63:626–628.Google Scholar
27. Jeffree, C. E., Baker, E. A., and Holloway, P. J. 1975. Ultrastructure and recrystallization of plant epicuticular waxes. New Phytol. 75: 539–549.Google Scholar
28. Jeffree, C. E., Baker, E. A., and Holloway, P. J. 1976. Origins of the fine structure of plant epicuticular waxes. Pages 119–158 in Dickinson, C. H. and Preece, T. F., eds. Microbiology of Aerial Plant Surfaces. Academic Press, London.Google Scholar
29. Kolattukudy, P. E. 1965. Biosynthesis of wax in Brassica oleracea . Biochemistry 4:1844–1855.Google Scholar
30. McWhorter, C. G. and Barrentine, W. L. 1988. Spread of paraffinic oil on leaf surfaces of johnsongrass (Sorghum halepense). Weed Sci. 36: 111–117.Google Scholar
31. McWhorter, C. G. and Paul, R. N. 1989. The involvement of silica cells in the production of wax filaments in johnsongrass (Sorghum halepense) leaves. Weed Sci. 37:458–470.Google Scholar
32. Norris, R. F. and Bukovac, M. J. 1972. Influence of cuticular waxes on penetration of pear leaf cuticle by 1-naphthalene-acetic acid. Pestic. Sci. 3:705–708.Google Scholar
33. Sargent, C. 1976. The in situ assembly of cuticular waxes. Planta 129: 123–126.CrossRefGoogle Scholar
34. Smith, L. C., Pownall, H., and Gotto, A. M. Jr. 1978. The plasma lipoproteins: structure and metabolism. Annu. Rev. Biochem. 47: 751–777.CrossRefGoogle ScholarPubMed
35. Von Wettstein-Knowles, P. 1974. The ultrastructure and origin of epicuticular wax tubes. J. Ultrastruct. Res. 46:483–498.Google Scholar
36. Winstel, R. and Rentschler, I. 1975. Elektronenmikroskopische Untersuchungen der Blattoberflache verschiedener Futterpflanzen. Micron 6:1–9.Google Scholar
37. Whitehouse, P. and Holloway, P. J. 1982. The epicuticular wax of wild oats in relation to foliar entry of the herbicides dichlofop-methyl and difenzoquat. Pages 315–330 in Cutler, D. F., Alvin, K. L., and Price, C. E., eds. The Plant Cuticle. Academic Press, London.Google Scholar
38. Zelitch, I. 1971. Photosynthesis, Photorespiration, and Plant Productivity. Academic Press, New York. 347 pp.Google Scholar