Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-22T19:31:49.288Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular Analysis of Hexazinone-Resistant Shepherd's-Purse (Capsella bursa-pastoris) Reveals a Novel psbA Mutation

Published online by Cambridge University Press:  20 January 2017

Alejandro Perez-Jones ,
Suphannika Intanon  and
Carol Mallory-Smith
Alejandro Perez-Jones
Affiliation:
Department of Crop and Soil Science, Oregon State University, 107 Crop Science Building, Corvallis, OR 97331
Suphannika Intanon
Affiliation:
Department of Crop and Soil Science, Oregon State University, 107 Crop Science Building, Corvallis, OR 97331
Carol Mallory-Smith*
Affiliation:
Department of Crop and Soil Science, Oregon State University, 107 Crop Science Building, Corvallis, OR 97331
*
Corresponding author's E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

A population of shepherd's-purse suspected to be resistant to the triazinone herbicide hexazinone, a photosystem II (PS II) inhibitor, was collected from an alfalfa field in 2007 in Oregon. A whole-plant, dose–response assay confirmed that the putative-resistant population was highly resistant to hexazinone. The resistant population was 22-fold more resistant to hexazinone than the susceptible population. However, the hexazinone-resistant population was susceptible to other PS II-inhibiting herbicides, including atrazine, diuron, and terbacil. DNA sequence analysis of the chloroplast psbA gene encoding the D1 protein of PS II, the target site of PS II inhibitors, identified a point mutation from Phe to Ile at position 255 in the hexazinone-resistant population. Single- and double-point mutations at position 255, which is located in the QB binding niche of the D1 protein, were previously reported in Chlamydomonas reinhardtii, Synechococcus species, and Synechocystis species after site-directed mutagenesis and were associated with decreased binding of PS II inhibitors. To our knowledge, this is the first report of a mutation of the psbA gene at Phe255 in a field-selected, herbicide-resistant plant.

Keywords

Atrazinediuronhexazinonemetribuzinterbacilalfalfa, Medicago sativa L. MEDSAshepherd's-purse, Capsella bursa-pastoris (L.) Medik CAPBP.PS II inhibitorsphotosynthesispsbAhexazinone-resistance
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 57 , Issue 6 , December 2009 , pp. 574 - 578
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Ajlani, G., Kirilovsky, D., Picaud, M., and Astier, C. 1989. Molecular analysis of psbA mutations responsible for various herbicide resistant phenotypes in Synechocystis . Plant Mol. Biol. 13:469–479.Google Scholar
Devine, M. D. and Eberlein, C. V. 1997. Physiological, biochemical and molecular aspects of herbicide resistance based on altered target sites. Pages 159–185. In Roe, R. M., Burton, J. D., and Kuhr, R. J. Herbicide Activity: Toxicology, Biochemistry and Molecular Biology. Amsterdam, The Netherlands IOS.Google Scholar
Dumont, M. and Tardif, F. J. 2002. Resistance to linuron in a Powell's amaranth population. Weed Sci. Soc. Am. Abstr. 42:49–50.Google Scholar
Eberlein, C. V., Al-Khatib, K., Guttieri, M. J., and Fuerst, P. 1992. Distribution and characteristics of triazine-resistant Powell amaranth (Amaranthus powellii) in Idaho. Weed Sci. 40:507–512.Google Scholar
Eleftherohorinos, I. G., Vasilakoglou, I. B., and Dhima, K. V. 2000. Metribuzin resistance in Amaranthus retroflexus and Chenopodium album in Greece. Weed Sci. 48:69–74.Google Scholar
Erickson, J. M., Pfister, K., Rahire, M., Togasaki, R., Mets, L., and Rochaix, J. D. 1989. Molecular and biophysical analysis of herbicide-resistant mutants of Chlamydomonas reinhardtii: structure-function relationship of the photosystem II D1 polypeptide. Plant Cell. 1:361–371.Google Scholar
Fuerst, E. P. and Norman, M. A. 1991. Interaction of herbicides with photosynthetic electron transport. Weed Sci. 39:458–464.Google Scholar
Gressel, J. 2002. Molecular Biology of Weed Control. London Taylor and Francis.Google Scholar
Gronwald, J. W. 1994. Resistance to photosystem II inhibiting herbicides. Pages 27–60. In Powles, S. B. and Holtum, J. A. M. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL Lewis.Google Scholar
Heap, I. 2009. International Survey of Herbicide Resistant Weeds. http://www.weedscience.org. Accessed: April 6, 2009.Google Scholar
Hess, F. D. 2000. Light dependent herbicides: an overview. Weed Sci. 48:160–170.Google Scholar
Hirschberg, J. and McIntosh, L. 1983. Molecular basis of herbicide resistance in Amaranthus hybridus L. Science. 222:1346–1349.Google Scholar
Holt, J. S. and Thill, D. C. 1994. Growth and productivity of resistant plants. Pages 299–316. In Powles, S. B. and Holtum, J. A. M. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL Lewis.Google Scholar
Jasieniuk, M., Brule-Babel, A. L., and Morrison, I. N. 1996. The evolution and genetics of herbicide resistance in weeds. Weed Sci. 44:176–193.Google Scholar
Knezevic, S. Z., Streibig, J. C., and Ritz, C. 2007. Utilizing R software package for dose–response studies: the concept and data analysis. Weed Technol. 21:840–848.Google Scholar
Masabni, J. G. and Zandstra, B. H. 1999. A serine-to-threonine mutation in linuron-resistant Portulaca oleracea . Weed Sci. 47:393–400.Google Scholar
Mechant, E., Demarez, T., Hermann, O., and Bulcke, R. 2008. An alanine-to-valine mutation in triazinone-resistant Chenopodium album L. Pages 92. in. Proceedings of the 5th International Weed Science Congress. Vancouver, BC, Canada International Weed Science Society.Google Scholar
Mengistu, L. W., Christoffers, M. J., and Lym, R. G. 2005. A psbA mutation in Kochia scoparia (L) Schrad from railroad rights-of-way with resistance to diuron, tebuthiuron and metribuzin. Pest Manag. Sci. 61:1035–1042.Google Scholar
Mengistu, L. W., Mueller-Warrant, G. W., Liston, A., and Barker, R. E. 2000. psbA mutation (valine219 to isoleucine) in Poa annua resistant to metribuzin and diuron. Pest Manag. Sci. 56:209–217.Google Scholar
Oettmeier, W. 1999. Herbicide resistance and supersensitivity in photosystem II. Cell. Mol. Life Sci. 55:1255–1277.Google Scholar
Oettmeier, W. and Hilp, U. 1991. Structure–activity relationship of triazinone herbicides on resistant weeds and resistant Chlamydomonas reinhardtii . Pestic. Sci. 33:399–409.Google Scholar
Ohad, N. and Hirschberg, J. 1992. Mutations in the D1 subunit of photosystem II distinguish between quinone and herbicide binding sites. Plant Cell. 4:273–282.Google Scholar
Park, K. W. and Mallory-Smith, C. A. 2006a. psbA mutation (Asn266 to Thr) in Senecio vulgaris L. confers resistance to several PS II-inhibiting herbicides. Pest Manag. Sci. 62:880–885.Google Scholar
Park, K. W. and Mallory-Smith, C. A. 2006b. psbA mutation in Amaranthus retroflexus confers resistance to several PS II–inhibiting herbicides. GenBank DQ8873759.Google Scholar
Preston, C. and Mallory-Smith, C. A. 2001. Biochemical mechanisms, inheritance, and molecular genetics of herbicide resistance in weeds. Pages 23–60. In Powles, S. B. and Shaner, D. L. Herbicide Resistance and World Grains. Boca Raton, FL CRC.Google Scholar
Rochaix, J. D. and Erickson, J. 1988. Function and assembly of photosystem II: genetic and molecular analysis. Trends Biochem. Sci. 13:56–60.Google Scholar
Ryan, G. F. 1970. Resistance of common groundsel to simazine and atrazine. Weed Sci. 18:614–616.Google Scholar
Seefeldt, S., Jensen, J. E., and Fuerst, E. P. 1995. Log–logistic analysis of herbicide dose–response relationships. Weed Technol. 9:218–225.Google Scholar
Smeda, R. and Vaughn, K. C. 1997. Mechanisms of resistance to herbicides. Pages 79–123. In Sjut, V. Molecular Mechanisms of Resistance to Agrochemicals. Berlin Springer.Google Scholar
Stanek, R. and Lipecki, J. 1991. Resistance to triazines of Capsella bursa-pastoris (L.) Med. is located in chloroplast. Resist. Pest Manag. 3:27.Google Scholar
Streibig, J. C., Rudemo, M., and Jensen, J. E. 1993. Dose–response curves and statistical models. Pages 29–56. In Streibig, J. C. and Kudsk, P. Herbicide Bioassays. Boca Raton, FL CRC.Google Scholar
Trebst, A. 1991. The molecular basis of resistance of photosystem II herbicides. Pages 145–164. In Casely, J. C., Cussans, G. W., and Atkin, R. K. Herbicide Resistance in Weeds and Crops. Oxford Butterworth-Heinemann.Google Scholar
Trebst, A. 1996. The molecular basis of plant resistance to photosystem II herbicides. Pages 44–51. In Brown, T. M. Molecular Genetics and Evolution of Pesticide Resistance. Washington, DC American Chemical Society.Google Scholar