Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-22T06:58:28.706Z Has data issue: false hasContentIssue false

A Mist Chamber for the Culture of Plants or Explant Segments

Published online by Cambridge University Press:  12 June 2017

C. A. Beasley
Affiliation:
Stanford Research Institute, Southern California Laboratories, South Pasadena, California
W. P. Fox
Affiliation:
Stanford Research Institute, Southern California Laboratories, South Pasadena, California

Abstract

Versatile mist chambers, framed with aluminum and glass and having access from top and sides, permit culture of an extensive number of, and different types of, vegetative plant organs as well as seeds and plant pathogens. Pneumatic atomizing nozzles are staggered to permit division of the chamber, and the spray emitted can be regulated from each nozzle. The amount of water vapor emitted is regulated by changing the air pressure or siphon height. Gauge-monitored pressure regulators and solenoid valves control the air and spray system. Timing devices can be used to inject water vapor at desired intervals. Air passing through a condensation tube is recycled past cone heaters, providing temperature from ambient to 90 F, and a duct and baffle system provides uniformity of temperature throughout. A combination recorder and controller maintains the desired temperature.

Type
Research Article
Copyright
Copyright © 1970 Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Johnson, B. G. and Buchholtz, K. P. 1961. An in vitro method of evaluating the activity of buds on the rhizomes of quackgrass (Agropyron repens [L.] Beauv.). Weeds 9:600606.Google Scholar
2. Beasley, C. A. 1970. Development of axillary buds from johnsongrass rhizomes. Weed Sci. 18:218222.Google Scholar