Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-22T19:26:23.596Z Has data issue: false hasContentIssue false

Metabolism of Food Reserves in Germinating Velvetleaf

Published online by Cambridge University Press:  12 June 2017

J. A. Mulliken
Affiliation:
Department of Agronomy, University of Wisconsin, Madison, Department of Biology, Texas A & M University, College Station, Texas
C. A. Kust
Affiliation:
, University of Wisconsin, Madison
L. E. Schrader
Affiliation:
, University of Wisconsin, Madison

Abstract

Endosperm dry weight, protein, and fat losses accompanied rapid radicle growth of velvetleaf (Abutilon theophrasti Medic.) between 12 and 36 hr of germination at 31 C. Cotyledonary reserves were mobilized after 36 hr. Isocitrate lyase activity sedimented with a particulate fraction in varying degrees, but maximal activity developed at times coincident with fat mobilization. Respiration of excised endosperms reached maximal rates shortly after radicle emergence. The actions of hydrogen cyanide, carbon monoxide, and 2,4-dinitrolphenol indicated that respiration of endosperms excised from imbibed and germinated seed was due to cytochrome oxidase activity, and was coupled to phosphorylation.

Type
Research Article
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Beevers, H. 1961. Metabolic production of sucrose from fat. Nature 191:433436.Google Scholar
2. Carpenter, D. W. and Beevers, H. 1959. Distribution and properties of isocitratase in plants. Plant Physiol. 34:403409.CrossRefGoogle Scholar
3. Christiansen, M. N. 1967. Periods of sensitivity to chilling in germinating cotton. Plant Physiol. 42:431433.Google Scholar
4. Dole, V. P. and Meinertz, H. 1960. Microdetermination of long-chain fatty acids in plasma and tissues. J. Biol. Chem. 235:25952599.Google Scholar
5. Earle, F. R. and Jones, Q. 1962. Analyses of seed samples from 113 plant families. Econ. Bot. 16:221250.Google Scholar
6. Gambhir, I. R. and Joshi, S. S. 1952. Chemical examination of the seeds of Abutilon indicum . J. Ind. Chem. Soc. 29: 451454.Google Scholar
7. Hackett, D. P. 1964. Enzymes of terminal respiration, p. 653. In Linskens, H. F., Sanwal, B. D. and Tracey, M. V. (ed.) Modern methods of plant analysis, Vol. 7. Springer-Verlag, Berlin.Google Scholar
8. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265276.Google Scholar
9. Jacks, T. J. and Alldridge, N. A. 1967. Use of mercuric acetate to remove cysteine interference in the isocitrate lyase assay Anal. Biochem. 18:378381.Google Scholar
10. LaCroix, L. J. and Staniforth, D. W. 1964. Seed dormancy in velvetleaf. Weeds 12:171174.Google Scholar
11. Mulliken, J. A. and Kust, C. A. 1970. Germination of velvetleaf. Weed Sci. 18: (In Press).Google Scholar
12. Reeves, R. G. 1936. Comparative anatomy of the seeds of cotton and other Malvaceous plants. I. Malveae and Ureneae. Am. J. Bot. 23:291296.Google Scholar
13. Robbie, W. A. 1944. An improved phenolphthalin technique for the microdetermination of cyanide. Arch. Biochem. 5:4958.Google Scholar
14. Rossi-Fanelli, A., Antonini, E., Brunori, H., Bruggesi, M. R., Caputo, A. and Satariani, F. 1964. Isolation of a monodisperse protein fraction from cottonseeds. Biochem. Biophys. Res. Comm. 15:110115.Google Scholar
15. Umbreit, W. W., Burris, R. H. and Stauffer, J. F. 1964. Manometric Techniques, 4th ed. Burgess Pub., Minneapolis. 338 p.Google Scholar
16. White, H. B. Jr. 1958. Fat utilization and composition in germinating cotton seeds. Plant Physiol. 33:218226.CrossRefGoogle ScholarPubMed
17. Winter, D. M. 1960. The development of the seed of Abutilon theophrasti. I. Ovule and embryo. Am. J. Bot. 47:814.CrossRefGoogle Scholar
18. Winter, D. M. 1960. The development of the seed of Abutilon theophrasti. II. Seed coat. Am. J. Bot. 47:157162.Google Scholar