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Interspecific hybridization and gene flow of ALS resistance in Amaranthus species

Published online by Cambridge University Press:  20 January 2017

Aaron S. Franssen ,
Daniel Z. Skinner ,
Kassim Al-Khatib ,
Michael J. Horak  and
Peter A. Kulakow
Aaron S. Franssen
Affiliation:
Department of Agronomy, Kansas State University, Manhattan KS 66502
Daniel Z. Skinner
Affiliation:
USDA-ARS, Department of Agronomy, Kansas State University, Manhattan, KS 66502
Michael J. Horak
Affiliation:
Monsanto Co., St. Louis, MO 63141
Peter A. Kulakow
Affiliation:
Department of Agronomy, Kansas State University, Manhattan KS 66502
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Abstract

Several inbred lines of acetolactate synthase (ALS)-inhibiting herbicide-resistant (ALS-R) Palmer amaranth and ALS-susceptible (ALS-S) common waterhemp were developed in the greenhouse. Interspecific hybrids were obtained by allowing several ALS-S common waterhemp females to be pollinated only by ALS-R Palmer amaranth in a growth chamber. Putative hybrid progeny were treated with an ALS-inhibiting herbicide, and the hybrid nature verified using a polymorphism found in the parental ALS gene. Polymerase chain reaction (PCR) was used to amplify a region of the ALS gene in both parental plants and putative hybrids. Restriction enzyme digestion of the ALS-R Palmer amaranth PCR fragment resulted in two smaller fragments, whereas the PCR fragment in the ALS-S common waterhemp was not cut. Restriction digestion of the putative hybrid PCR fragment showed a combination of ALS-R Palmer amaranth double fragments and an ALS-S common waterhemp single fragment. Approximately 4 million flowers were present on 11 common waterhemp females and produced about 44,000 seeds that appeared viable. From the approximately 3,500 putative hybrid seedlings that were screened, 35 were confirmed as hybrids using herbicide resistance as a phenotypic and molecular marker. The data collected here verify that interspecific hybridization does occur between these two species, and even at a low rate, it could contribute to the rapid spread of ALS resistance in these species.

Keywords

Imazethapyrcommon waterhemp, Amaranthus rudis Sauer AMATAPalmer amaranth, Amaranthus palmeri S. Wats AMAPAMolecular markerspolymerase chain reactionrestriction endonuclease
Type
Research Article
Information
Weed Science , Volume 49 , Issue 5 , October 2001 , pp. 598 - 606
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Barkely, T. M., ed. 1986. Flora of the Great Plains. Lawrence, KS: Great Plains Flora Association, University Press of Kansas. pp. 179–184.Google Scholar
Beyer, E. M. Jr., Duffy, M. J., Hays, J. V., and Schlueter, D. D. 1988. Sulfonylureas. Pages 117–189 In Kearney, P. C. and Kaufman, D. D., eds. Herbicides: Chemistry, Degradation, and Mode of Action. New York: Marcel Dekker.Google Scholar
Dellaporta, S., Woods, J., and Hicks, J. 1983. A plant DNA minipreparation, version II. Plant Mol. Biol. Rep. 1:19–21.Google Scholar
Foes, M. J., Liu, L., Tranel, P. J., Wax, L. W., and Stoller, E. W. 1998. A biotype of common waterhemp (Amaranthus rudis) resistant to triazine and ALS herbicides. Weed Sci. 46:514–520.CrossRefGoogle Scholar
Gossett, B. J., Murdock, E. C., and Toler, J. E. 1992. Resistance of Palmer amaranth (Amaranthus palmeri) to dinitroaniline herbicides. Weed Technol. 6:587–591.Google Scholar
Guttieri, M. J., Eberlein, C. V., Mallory-Smith, C. A., Thill, D. C., and Hoffman, D. L. 1992. DNA sequence variation in domain A of the acetolactate synthase genes of herbicide-resistant and -susceptible weed biotypes. Weed Sci. 40:670–676.Google Scholar
Heap, I. 2000. International survey of herbicide resistant weeds. Available online at http://www.weedscience.com. Last accessed January 2001.Google Scholar
Horak, M. J. and Peterson, D. E. 1995. Biotypes of Palmer amaranth (Amaranthus palmeri) and common waterhemp (Amaranthus rudis) are resistant to imazethapyr and thifensulfuron. Weed Technol. 9:192–195.Google Scholar
Horak, M. J., Peterson, D. E., Chessman, D. J., and Wax, L. M. 1994. Pigweed identification: A pictorial guide to the common pigweeds of the Great Plains. Manhattan, KS: Kansas State University Cooperative Extension Service Publication S80. 11 p.Google Scholar
Keeley, P. E., Carter, C. H., and Thullen, R. M. 1987. Influence of planting date on growth of Palmer amaranth (Amaranthus palmeri). Weed Sci. 35:199–204.Google Scholar
Kerr, L. A. and Kelch, W. J. 1998. Pigweed (Amaranthus retroflexus) toxicosis in cattle. Vet. Human Toxicol. 40:216–218.Google Scholar
Klingaman, T. E. and Oliver, L. R. 1994. Palmer amaranth (Amaranthus palmeri) interference in soybean (Glycine max). Weed Sci. 42:523–527.Google Scholar
Knezevik, S. Z., Horak, M. J., and Vanderlip, R. L. 1997. Relative time of redroot pigweed (Amaranthus retroflexus) emergence is critical in pigweed-sorghum [Sorghum bicolor (L.) Moench] competition. Weed Sci. 45:502–508.Google Scholar
Kulakow, P. A. 1987. Genetics of grain amaranths. II. The inheritance of determinance, panicle orientation, dwarfism, and embryo color in Amaranthus caudatus . J. Hered. 78:293–297.Google Scholar
Kulakow, P. A., Hauptli, H., and Jain, S. K. 1985. Genetics of grain amaranths. I. Mendelian analysis of six color characteristics. J. Hered. 76:27–30.Google Scholar
Mallory-Smith, C. A., Thill, D. C., and Dial, M. J. 1990. Identification of sulfonylurea herbicide-resistant prickly lettuce (Lactuca serriola). Weed Technol. 4:163–168.Google Scholar
Menges, R. M. 1987. Allelopathic effects of Palmer amaranth (Amaranthus palmeri) and other plant residues in soil. Weed Sci. 35:339–347.Google Scholar
Menges, R. M. 1988. Allelopathic effects of Palmer amaranth (Amaranthus palmeri) on seedling growth. Weed Sci. 36:325–328.Google Scholar
Murphy, S. D., Yankubu, Y., Weise, S. F., and Stanton, C. J. 1996. Effect on planting patterns and inter-row cultivation on competition between corn (Zea mays) and late emerging weeds. Weed Sci. 44:856–870.CrossRefGoogle Scholar
Pal, M. and Khoshoo, T. N. 1972. Evolution and improvement of cultivated amaranth. V. Inviability, weakness and sterility in hybrids. J. Hered. 63:78–82.Google Scholar
Poehlman, J. M. and Sleper, D. A. 1995. Breeding hybrid cultivars. Pages 200–215 In Breeding Field Crops. Ames, IA: Iowa State University Press.Google Scholar
Saari, L. L., Cotterman, J. C., and Thill, D. C. 1994. Resistance to acetolactate synthase inhibiting herbicides. Pages 83–139 In Powles, S. B. and Holtum, J.A.M., eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Roca, FL: CRC Press.Google Scholar
Sprague, C. L., Stoller, E. W., Wax, L. M., and Horak, M. J. 1997. Palmer amaranth (Amaranthus palmeri) and common waterhemp (Amaranthus rudis) resistance to selected ALS-inhibiting herbicides. Weed Sci. 45:192–197.CrossRefGoogle Scholar
Vizantinopoulos, S. N. and Katranis, N. 1998. Weed management of Amaranthus spp. in corn (Zea mays). Weed Technol. 12:145–150.Google Scholar
Wax, L. M. 1995. Pigweeds of the Midwest—distribution, importance and management. Proc. Integ. Crop Manag. 7:239–242.Google Scholar
Wetzel, D. K., Horak, M. J., Skinner, D. Z., and Kulakow, P. A. 1999. Transferal of herbicide resistance traits from Amaranthus palmeri to Amaranthus rudis . Weed Sci. 47:538–543.Google Scholar
Woodsworth, A. R., Rosen, B. A., and Bernasconi, P. 1996. Broadrange resistance to herbicides targeting acetolactate synthase (ALS) in a field isolate of Amaranthus sp. is conferred by a Trp to Leu mutation in ALS gene. Plant Physiol. 111:1353.Google Scholar