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Inhibition of Nitrite Reduction with Photosynthetic Inhibitors

Published online by Cambridge University Press:  12 June 2017

Lowell A. Klepper*
Affiliation:
Dep. of Agron., Univ. of Nebraska, Lincoln, NE 68503

Abstract

Using a modified in vivo nitrate reductase assay, all photosynthetic inhibitors tested were shown to effectively block light-dependent nitrite reduction in green leaf tissue. Nitrate reduction continued so that nitrite accumulated. Nitrite, vacuum infiltrated into leaf tissue, disappeared linearly with time when incubated in the light. Nitrite, vacuum infiltrated with a photosynthetic inhibitor, did not disappear but accumulated to even higher levels. The degree of inhibition of nitrite reduction was a function of herbicide concentration and time of incubation.

Type
Research Article
Copyright
Copyright © 1975 by the Weed Science Society of America 

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References

Literature Cited

1. Joy, K.W. and Hageman, R.H. 1966. The purification and properties of nitrite reductase from higher plants and its dependence on ferredoxin. Biochem. J. 100:263273.Google Scholar
2. Klepper, L. 1974. A mode of action of herbicides: Inhibition of the normal process of nitrite reduction. Nebraska Exp. Sta. Res. Bull. 259. 42 pp.Google Scholar
3. Klepper, L., Flesher, D., and Hageman, R.H. 1971. Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves. Plant Physiol. 48:580590.CrossRefGoogle ScholarPubMed
4. Moreland, D.E. and Hill, K.L. 1962. Interference of herbicides with the Hill reaction of isolated chloroplasts. Weeds 10:229236.Google Scholar
5. Moreland, D.E., Gentner, W.A., Hilton, J.L., and Hill, K.L. 1959. Studies on the mechanism of herbicidal action of 2-chloro-4,6-bis(ethylamino)-s-triazine. Plant Physiol. 34:432435.CrossRefGoogle ScholarPubMed
6. Neyra, C.A. and Hageman, R.H. 1975. Dependence of nitrite reduction on electron transport in chloroplasts. Plant Physiol. 54: (in press).Google Scholar
7. Pyne, W.J., Szabo, S.S., and Holm, R.E. 1975. Synthesis and herbicidal activity of pyrrolidincarboxanilides. J. Agr. Food Chem. 23: (in press).Google Scholar
8. Ritenour, G.L., Joy, K.W., Bunning, J.J., and Hageman, R.H. 1967. Intracellular location of nitrate reductase, nitrite reductase and glutamic dehydrogenase in green leaf tissue. Plant Physiol. 42:233237.Google Scholar
9. Vanecko, S. and Varner, J. 1955. Studies of nitrite metabolism in higher plants. Plant Physiol. 30:388390.Google Scholar