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The in Vitro Culture of Excised Ovules from Velvetleaf (Abutilon theophrasti)

Published online by Cambridge University Press:  12 June 2017

Laura K. Thompson ,
Gerald R. Leather  and
Maynard G. Hale
Laura K. Thompson
Affiliation:
Dep. Plant Pathol. and Physiol., Virginia Polytech. Inst. and State Univ., Blacksburg, VA 24061
Gerald R. Leather
Affiliation:
Weed Sci. Res., Agric. Res. Serv., U.S. Dep. Agric., Bldg. 1301, Ft. Detrick, Frederick, MD 21701
Maynard G. Hale
Affiliation:
Dep. Plant Pathol. and Physiol., Virginia Polytech. Inst. and State Univ., Blacksburg, VA 24061
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Abstract

A culture medium and environmental conditions were selected for ovule culture of velvetleaf (Abutilon theophrasti Medic. ♯4 ABUTH), which allowed development parallel to that occurring in vivo. Of the six nutrient media evaluated, the most suitable one contained an organic nitrogen source and was of high osmotic potential. A liquid medium with a pH of 5 to 7, and culture for 14 days at 25 C under green light (1.3 μmole·m-2·-1) encouraged good embryo and ovule development. No difference was recorded between polyethylene glycol 20000 and mannitol in the medium used for dehydration and subsequent imbibition of cultured mature ovules. To investigate the effects of maternal tissues, surgical alterations were performed on the testa and funiculus of immature velvetleaf ovules. The intact testa appears to be a barrier to nutrient absorption and reduces the fresh and dry weights and embryo length. Ovule and embryo development was significantly better if either the testa was nicked or the funiculus removed.

Keywords

Ovule cultureembryo developmentseed developmentseed dormancyABUTH
Type
Weed Biology and Ecology
Information
Weed Science , Volume 32 , Issue 6 , November 1984 , pp. 792 - 797
Copyright
Copyright © 1984 by the Weed Science Society of America 

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References

Literature Cited

1. Adams, C. A. and Rinne, R. W. 1981. Seed maturation in soybeans (Glycine max L. Merr.) is independent of seed mass and of the parent plant, yet is necessary for production of viable seeds. J. Exp. Bot. 32:615–620.Google Scholar
2. Beasley, C. A. and Ting, I. P. 1973. The effects of plant growth substances on in vitro fiber development from fertilized cotton ovules. Am. J. Bot. 60:130–139.Google Scholar
3. Brown, R. H. 1974. Biology and control of velvetleaf (Abutilon theophrasti Medic). Pages 46–48 in Minutes of the 28th Meeting, Canada Weed Committee, Eastern Section.Google Scholar
4. Cameron-Mills, V. and Duffus, C. M. 1980. The influence of nutrition on embryo development and germination. Cereal Res. Commun. 8:143–149.Google Scholar
5. Delouche, J. C. 1980. Environmental effects on seed development and seed quality. HortScience 15:13–18.Google Scholar
6. Delouche, J. C., Still, T. W., Raspt, M., Lienhard, M. 1962. The tetrazolium test for seed viability. Miss. State Univ. Agric. Exp. Stn. Tech. Bull. 51.Google Scholar
7. Dietrick, K. 1924. Ueber die kultur von embryonene ausserhalb des samens. Flora 117:379–417.Google Scholar
8. Gutterman, Y. 1974. The influence of the photoperiod regime and red-far red light treatments of Portulaca oleracea L. plants on the germinability of their seeds. Oecologia 17:27–38.CrossRefGoogle ScholarPubMed
9. Hsu, F. C. and Obendorf, R. L. 1982. Compositional analysis of in vitro matured soybean seeds. Plant Sci. Lett. 27:129–135.Google Scholar
10. Ihle, J. N. and Dure, L. 1969. Synthesis of a protease in germinating cotton cotyledons catalyzed by mRNA synthesized during embryogenesis. Biochem. Biophys. Res. Commun. 36:205–210.Google Scholar
11. Ihle, J. N. and Dure, L. 1970. Hormonal regulation of translation, inhibition requiring RNA synthesis. Biochem. Biophys. Res. Commun. 38:995–1001.Google Scholar
12. Karssen, C. M. 1970. The light promoted germination of the seeds of Chenopodium album L. III. Effect of the photoperiod during growth and development of the plants on the dormancy of the produced seeds. Acta Bot. Neerl. 19:81–94.Google Scholar
13. LaCroix, L. J. and Staniforth, D. W. 1964. Seed dormancy in velvetleaf. Weeds 12:171–174.Google Scholar
14. Linsmaier, E. M. and Skoog, F. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18:100–127.Google Scholar
15. Millerd, A., Spencer, D., Dudman, W. F., and Stiller, M. 1975. Growth of immature pea cotyledons in culture. Aust. J. Plant Physiol. 2:51–59.Google Scholar
16. Matsuzawa, Y. 1978. Studies on the interspecific hybridization in the genus Brassica. I. Effects of temperature on the development of hybrid embryos and the improvement of crossability of ovary culture in interspecific cross, Brassica campestris X Brassica oleracea . Jpn. J. Breed. 28:186–196.Google Scholar
17. Nitsch, J. P. 1951. Growth and development in vitro of excised ovaries. Am. J. Bot. 38:566–577.Google Scholar
18. Norstog, K. 1966. Influence of nutritional and physical factors on growth of cultured barley embryos. Am. J. Bot. 53:613–614.Google Scholar
19. Obendorf, R. L. and Wettiaufer, S. H. 1983. Precocious germination during in vitro growth of soybean seeds. Plant Physiol. 72(Suppl.): 88.Google Scholar
20. Rijven, A. H. G. C. 1952. In vitro studies on the embryo of Capsella bursa-pastoris . Acta Bot. Neerl. 1:157–200.Google Scholar
21. Sanchez, R. A., Eyherabide, G., and deMiguel, L. 1981. The influence of irradiance and water deficit during fruit development on seed dormancy of Datura ferox L. Weed Res. 21:127–132.Google Scholar
22. Srivastava, P. S., Varga, A., and Bruinsma, J. 1980. Growth in vitro of fertilized ovules of pea, Pisum sativum, L., with and without pods. Z. Pflanzenphysiol. 98:347–355.Google Scholar
23. Stewart, J. M. D. 1979. Use of ovule cultures to obtain interspecific hybrids of Gossypium . Pages 44–56 in Barber, J. J., ed. Plant Tissue Culture. New Orleans, LA, Symposium: South. Sec. Am. Soc. Plant Physiol.Google Scholar
24. Stewart, J. M. and Hsu, C. L. 1977. In vitro embryo culture and seedling development of cotton (Gossypium hirsutum L.). Planta 137:113–117.Google Scholar
25. Thomas, J. F. and Raper, C. 1979. Germinability of tobacco seed as affected by culture of the mother plant. Agron. J. 71:694–695.Google Scholar
26. Thompson, J. F., Madison, J. T., and Muenster, A. M. 1977. In vitro culture of immature cotyledons of soybean (Glycine max L. Merr. J. Ann. Bot. 41:29–39.Google Scholar
27. Tomar, D. P. S., Deshmukh, P. S., and Sinha, S. K. 1979. Importance of sepals in fruit and seed development in linseed (Linum usitatissimum L.). Euphytica 28:739–745.Google Scholar
28. Van Overbeek, J., Siu, R., and Haagen-Smith, A. J. 1944. Factors affecting the growth of Datura embryos in vitro . Am. J. Bot. 31:219–224.CrossRefGoogle Scholar
29. Werker, E. 1980. Seed dormancy as explained by the anatomy of embryo envelopes. Isr. J. Bot. 29:22–44.Google Scholar
30. Winter, D. 1960. The development of the seed of Abutilon theophrasti. I. Ovule and Embryo. Am. J. Bot. 47:8–14.Google Scholar
31. Winter, D. 1960. The development of the seed of Abutilon theophrasti. II. Seed Coat. Am. J. Bot. 47:157–162.Google Scholar