Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-22T13:52:57.129Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification of Two Mechanisms of Sulfonylurea Resistance Within One Population of Rigid Ryegrass (Lolium rigidum) Using a Selective Germination Medium

Published online by Cambridge University Press:  12 June 2017

Michael W. M. Burnet ,
John T. Christopher ,
Joseph A. M. Holtum  and
Stephen B. Powles
Michael W. M. Burnet
Affiliation:
Dep. Crop Prot., Waite Agric. Res. Inst., Univ. Adelaide, Glen Osmond, South Australia 5064
John T. Christopher
Affiliation:
Dep. Crop Prot., Waite Agric. Res. Inst., Univ. Adelaide, Glen Osmond, South Australia 5064
Joseph A. M. Holtum
Affiliation:
Dep. Crop Prot., Waite Agric. Res. Inst., Univ. Adelaide, Glen Osmond, South Australia 5064
Stephen B. Powles
Affiliation:
Dep. Crop Prot., Waite Agric. Res. Inst., Univ. Adelaide, Glen Osmond, South Australia 5064
Get access Rights & Permissions [Opens in a new window]

Abstract

A biotype of rigid ryegrass (Lolium rigidum Gaudin biotype VLR69) resistant to some ALS inhibitors was characterized to determine the mechanisms of resistance to the sulfonylurea herbicides. The biotype had a high level of resistance to chlorsulfuron (20×) and triasulfuron (25×), and an intermediate level of resistance to imazaquin (7×) and sulfometuron (7.5×) but exhibited a low level of resistance to imazapyr (2.5×). At 60 to 90 g ai ha-1 sulfometuron, 4% of the population survived without apparent herbicidal effect The same response to sulfometuron was also observed when seeds of the resistant biotype VLR69 were germinated on agar containing sulfometuron. At 27 nM sulfometuron, 4% of the seeds germinated and grew normally while the growth of the bulk of the population was retarded. This differential response to sulfometuron at the seedling stage allowed selection of sulfometuron-resistant individuals from the population. Activity of ALS extracted from these sulfometuron-resistant plants was less sensitive to inhibition by chlorsulfuron than ALS extracted from plants considered sulfometuron susceptible in the same system. Unselected plants from the VLR69 population were able to detoxify chlorsulfuron more rapidly than susceptible VLR1 plants. It is apparent that there are at least two mechanisms of sulfonylurea resistance in the resistant biotype VLR69 which occur at different frequencies within the population. These data show that more than one mechanism or resistance may develop in response to herbicide selection pressure and that the resulting populations are not necessarily homogeneous.

Keywords

Chlorsulfuron, 2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl]benzenesulfonamideimazapyr (±)-2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-3-pyridinecarboxylic acidimazaquin, 2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-3-quinolinecarboxylic acidsulfometuron, 2-[[[[(4,6-dimethyl-2-pyrimidinyl)amino]carbonyl]amino]-sulfonyl]benzoic acidtriasulfuron, 2-(2-chloroethoxy)-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)-amino]carboxyl]benzenesulfonamiderigid ryegrass, Lolium rigidum G. # LOLRIHerbicide resistancemultiple mechanismsALSchlorsulfuron metabolismimidazolinonesLOLRI
Type
Special Topics
Information
Weed Science , Volume 42 , Issue 3 , September 1994 , pp. 468 - 473
Copyright
Copyright © 1994 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Anderson, J. J., Priester, T. M., and Shalaby, L. M. 1989. Metabolism of metsulfuron-methyl in wheat and barley. J. Agric. Food Chem. 37:1429–1434.CrossRefGoogle Scholar
2. Beyer, E. M., Duffy, M. J., Hay, J. V., and Schlueter, D. D. 1988. Sulfonylureas. Pages 118–169 in Kearney, P. C. and Kaufman, D. D., eds. Herbicides: Their Chemistry, Degradation and Mode of Action. Vol. 3. Marcel-Dekker, New York.Google Scholar
3. Burnet, M. W. M., Hart, Q., Holtum, J. A. M., and Powles, S. B. 1994. Resistance to nine herbicide classes in a biotype of rigid ryegrass (Lolium rigidum). Weed Sci. (in press).CrossRefGoogle Scholar
4. Burnet, M. W. M., Loveys, B. R., Holtum, J. A. M., and Powles, S. B. 1993. A mechanism of chlorotoluron resistance in Lolium rigidum . Planta 190:182–189.CrossRefGoogle Scholar
5. Burnet, M. W. M., Loveys, B. R., Holtum, J. A. M., and Powles, S. B. 1993. Increased detoxification is a mechanism of simazine resistance in Lolium rigidum . Pestic. Biochem. Physiol. 46:207–218.CrossRefGoogle Scholar
6. Christopher, J. T., Powles, S. B., Holtum, J. A. M., and Liljegren, D. R. 1991. Cross-resistance to herbicides in annual ryegrass (Lolium rigidum): II. Chlorsulfuron resistance involves a wheat-like detoxification system. Plant Physiol. 95:1036–1043.CrossRefGoogle ScholarPubMed
7. Christopher, J. T., Powles, S. B., Liljegren, D. R., and Holtum, J. A. M. 1992. Resistance to acetolactate synthase inhibitors in annual ryegrass (Lolium rigidum) involves at least two mechanisms. Plant Physiol. 100:1909–1913.CrossRefGoogle ScholarPubMed
8. Cotterman, J. C. and Saari, L. L. 1992. Rapid metabolic inactivation is the basis for cross-resistance to chlorsulfuron in diclofop-methyl resistant rigid ryegrass (Lolium rigidum) SR4/84. Pestic. Biochem. Physiol. 43:182–192.CrossRefGoogle Scholar
9. Heap, I. and Knight, R. 1986. The occurrence of herbicide cross-resistance in a population of annual ryegrass, Lolium rigidum, resistant to diclofop-methyl. Aust. J. Agric. Res. 37:149–156.CrossRefGoogle Scholar
10. Heap, I. M. and Knight, R. 1990. Variations in herbicide cross-resistance among populations of annual ryegrass (Lolium rigidum) resistant to diclofop-methyl. Aust. J. Agric. Res. 41:121–128.CrossRefGoogle Scholar
11. Huppatz, J. L. and Casida, J. E. 1985. Acetohydroxyacid synthase inhibitors: N-phthalayl-L-valine anilide and related compounds. Z. Naturforsch. 40:652–656.CrossRefGoogle ScholarPubMed
12. LaRossa, R. A. and Schloss, J. V. 1984. The sulfonylurea herbicide sulfometuron methyl is an extremely potent and selective inhibitor of acetolactate synthase in Salmonella typhimurium . J. Biol. Chem. 259:8753–8757.CrossRefGoogle ScholarPubMed
13. Mallory-Smith, C. A., Thill, D. C., and Dial, M. J. 1990. Identification of herbicide-resistant prickly lettuce (Lactuca serriola). Weed Technol. 4:163–168.CrossRefGoogle Scholar
14. Matthews, J. M., Holtum, J. A. M., Liljegren, D. R., Furness, B., and Powles, S. B. 1990. Cross-resistance to herbicides in annual ryegrass (Lolium rigidum): I. Properties of the herbicide target enzymes acetyl-coenzyme A carboxylase and acetolactate synthase. Plant Physiol. 94:1180–1186.CrossRefGoogle ScholarPubMed
15. Meyer, A. M. and Muller, F. 1989. Triasulfuron and its selective behaviour in wheat and Lolium perenne . Proc. Br. Crop Prot. Conf.-Weeds. 1989. Vol. 3:441–443.Google Scholar
16. Primiani, M. M., Cotterman, J. C., and Saari, L. L. 1990. Resistance of kochia (Kochia scoparia) to sulfonylurea and imidazolinone herbicides. Weed Technol. 4:169–172.CrossRefGoogle Scholar
17. Ray, T. B. 1984. Site of action of chlorsulfuron: inhibition of valine and isoleucine synthesis in plants. Plant Physiol. 75:827–831.CrossRefGoogle Scholar
18. Saari, L. L., Cotterman, J. C., and Primiani, M. M. 1989. Mechanism of sulfonylurea herbicide resistance in the broadleaf weed, Kochia scoparia . Plant Physiol. 93:55–61.CrossRefGoogle Scholar
19. Saari, L. L., Cotterman, J. C., Smith, W. F., and Primiani, M. M. 1989. Sulfonylurea resistance in common chickweed, perennial ryegrass, and russian thistle. Pestic. Biochem. Physiol. 42:110–118.CrossRefGoogle Scholar
20. Shaner, D. L., Anderson, P. C., and Stidham, M. A. 1984. Imidazolinones, potent inhibitors of acetohydroxyacid synthase. Plant Physiol. 76:545–546.CrossRefGoogle ScholarPubMed
21. Shaner, D. L. and Robson, P. L. 1985. Absorption, translocation and metabolism of AC 252 214 in soybean (Glycine max), common cocklebur (Xanthium strumarium) and velvetleaf (Abutilon theophrasti). Weed Sci. 33:469–471.CrossRefGoogle Scholar
22. Sweetser, P. B. 1985. Safening of sulfonylurea herbicides to cereal crops: mode of herbicide antidote action. Proc. Br. Crop Prot. Conf.-Weeds: 1147–1154.Google Scholar
23. Sweetser, P. B., Schow, G. S., and Hutchison, J. M. 1982. Metabolism of chlorsulfuron by plants: biological basis for selectivity of a new herbicide for cereals. Pestic. Biochem. Physiol. 17:18–23.CrossRefGoogle Scholar
24. Westerfield, W. W. 1945. A colorimetric determination of blood acetoin. J. Biol. Chem. 161:495–502.CrossRefGoogle ScholarPubMed