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Host range of a deleterious rhizobacterium for biological control of downy brome

Published online by Cambridge University Press:  20 January 2017

Ann C. Kennedy ,
Bradley N. Johnson  and
Tami L. Stubbs
Bradley N. Johnson
Affiliation:
Land Management and Water Conservation Research Unit, USDA Agricultural Research Service, 215 Johnson Hall, Washington State University, Pullman, WA, 99164-6421
Tami L. Stubbs
Affiliation:
Land Management and Water Conservation Research Unit, USDA Agricultural Research Service, 215 Johnson Hall, Washington State University, Pullman, WA, 99164-6421
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Abstract

Pseudomonas fluorescens strain D7 (P. f. D7; NRRL B-18293) is a root-colonizing bacterium that inhibits downy brome (Bromus tectorum L. BROTE) growth. Before commercialization as a biological control agent, strain D7 must be tested for host plant specificity. Agar plate bioassays in the laboratory and plant–soil bioassays in a growth chamber were used to determine the influence of P. f. D7 on germination and root growth of 42 selected weed, cultivated or native plant species common in the western and midwestern United States. In the agar plate bioassay, all accessions of downy brome were inhibited by P. f. D7. Root growth of seven Bromus spp. was inhibited an average of 87% compared with that of controls in the agar plate bioassay. Root growth of non-Bromus monocots was reduced by 0 to 86%, and only 6 out of 17 plant species were inhibited 40% or greater. Among all plant species, only downy brome root growth from two accessions was significantly inhibited by P. f. D7 in plant–soil bioassays (42 and 64%). P. f. D7 inhibited root growth and germination in agar plate bioassays more than in plant–soil bioassays. Inhibition in plant–soil bioassays was limited to downy brome, indicating promise for P. f. D7 as a biocontrol agent that will not harm nontarget species.

Keywords

Downy bromeBromus tectorum L. BROTErhizobacteriumPseudomonas fluorescensBioassaygerminationrhizosphere
Type
Research Article
Information
Weed Science , Volume 49 , Issue 6 , December 2001 , pp. 792 - 797
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Alexander, M., ed. 1977. Microbiology of the rhizosphere. Pages 423–437 In Introduction to Soil Microbiology. 2nd ed. New York: J. Wiley.Google Scholar
Alstrom, S. 1987. Factors associated with detrimental effects of rhizobacteria on plant growth. Plant Soil 102:3–9.CrossRefGoogle Scholar
Bolton, H. Jr., Elliott, L. F., Gurusiddaiah, S., and Fredrickson, J. K. 1989. Characterization of a toxin produced by a rhizobacterial Pseudomonas sp. that inhibits wheat growth. Plant Soil 114:279–287.Google Scholar
Boyetchko, S. M. 1997. Principles of biological weed control with microorganisms. HortScience 32:201–205.Google Scholar
Brock, T. D. 1985. Prokaryotic population ecology. Pages 176–179 In Halvorson, H. O., Palmer, D., and Rogul, M., eds. Engineered Organisms in the Environment: Scientific Issues. Washington: American Society of Microbiologists.Google Scholar
Caesar, A. J., Campobasso, G., and Terragitti, G. 1999. Effects of European and U.S. strains of Fusarium spp. pathogenic to leafy spurge on North American grasses and cultivated species. Biol. Control 15:130–136.Google Scholar
Cherrington, C. A. and Elliott, L. F. 1987. Incidence of inhibitory pseudomonads in the Pacific Northwest. Plant Soil 101:159–165.CrossRefGoogle Scholar
Compeau, G., Al-Achi, B. J., Platsouka, E., and Levy, S. B. 1988. Survival of rifampicin-resistant mutants of Pseudomonas fluorescens and Pseudomonas putida in soil systems. Appl. Environ. Microbiol. 54:2432–2438.CrossRefGoogle Scholar
Fredrickson, J. K. and Elliott, L. F. 1985. Effects on winter wheat seedling growth by toxin-producing rhizobacteria. Plant Soil 83:399–409.CrossRefGoogle Scholar
Gurusiddaiah, S., Gealy, D. R., Kennedy, A. C., and Ogg, A. G. Jr. 1994. Isolation and characterization of metabolites from Pseudomonas fluorescens-D7 for control of downy brome (Bromus tectorum). Weed Sci. 42:492–501.CrossRefGoogle Scholar
Harper, S.H.T. and Lynch, J. M. 1980. Microbial effects on the germination and seedling growth of barley. New Phytol. 84:473–481.CrossRefGoogle Scholar
Kennedy, A. C., Elliott, L. F., Young, F. L., and Douglas, C. L. 1991. Rhizobacteria suppressive to the weed downy brome. Soil Sci. Soc. Am. J. 55:722–727.Google Scholar
Kennedy, A. C. and Kremer, R. J. 1996. Microorganisms in weed control strategies. J. Prod. Agric. 9:480–485.CrossRefGoogle Scholar
Klemmedson, J. O. and Smith, J. G. 1964. Cheatgrass (Bromus tectorum L.). Bot. Rev. 30:226–262.Google Scholar
Kloepper, J. W. and Schroth, M. N. 1981. Relationship of in vitro antibiosis of plant growth-promoting rhizobacteria to plant growth and the displacement of root microflora. Phytopathology 71:1020–1024.Google Scholar
Kremer, R. J. and Kennedy, A. C. 1996. Rhizobacteria as biocontrol agents of weeds. Weed Technol. 10:601–609.CrossRefGoogle Scholar
Krieg, N. R., ed. 1984. Pseudomonads. Pages 142–199 In Bergey's Manual of Systematic Bacteriology. Volume 1. Baltimore, MD: Williams and Wilkens.Google Scholar
Loper, J. E., Haack, E., and Schroth, M. N. 1985. Population dynamics of soil pseudomonads in the rhizosphere of potato (Solanum tuberosum L.). Appl. Environ. Microbiol. 49:416–422.CrossRefGoogle ScholarPubMed
Lynch, J. M. 1979. Microorganisms in their natural environments. I. The terrestrial environment. Pages 67–91 In Microbial Ecology: A Conceptual Approach. Oxford, Great Britain: Blackwell Scientific.Google Scholar
Madison, J. H. 1971. Turfgrass varieties. Pages 18–59 In Madison, J. H., ed. Practical Turfgrass Management. New York: Van Nostrand.Google Scholar
Marumoto, T., Anderson, J.P.E., and Domsch, K. H. 1982. Decomposition of 14C- and 15N-labelled microbial cells in soil. Soil Biol. Biochem. 14:461–467.Google Scholar
McVicker, M. H., ed. 1974. Important pasture grasses and legumes. Pages 51–92 In Approved Practices in Pasture Management. Danville, IL: Interstate.Google Scholar
Morrow, L. A. and Stahlman, P. W. 1984. The history and distribution of downy brome (Bromus tectorum) in North America. Weed Sci. 32 (Suppl. 1): 2–6.CrossRefGoogle Scholar
Peeper, T. F. 1984. Chemical and biological control of downy brome (Bromus tectorum) in wheat and alfalfa in North America. Weed Sci. 32 (Suppl. 1): 18–25.CrossRefGoogle Scholar
Sands, D. C. and Rovira, A. D. 1970. Isolation of fluorescent pseudomonads with a selective medium. Appl. Microbiol. 20:513–514.CrossRefGoogle ScholarPubMed
Scher, R. M., Kloepper, J. W., Singleton, C., Zaleska, I., and Laliberte, M. 1988. Colonization of soybean roots by Pseudomonas and Serratia sp.: relationship to bacterial motility, chemotaxis and generation time. Phytopathology 78:1055–1059.Google Scholar
Stanley, J. N. and Julien, M. H. 1999. The host range of Eccritotarsus catarinensis, a potential agent for the biological control of waterhyacinth (Eichhornia crassipes). Biol. Control 14:134–140.Google Scholar
Steel, R.G.D., Torrie, J. H., and Dickey, D. A., eds. 1997. Multiple comparisons. Pages 178–203 In Principles and Procedures of Statistics: A Biometrical Approach. New York: McGraw Hill.Google Scholar
Suslow, T. V. and Schroth, M. N. 1982. Role of deleterious rhizobacteria as minor pathogens in reducing crop growth. Phytopathology 72:111–115.CrossRefGoogle Scholar
TeBeest, D. O. and Templeton, G. E. 1985. Mycoherbicides: progress in the biological control of weeds. Plant Dis. 69:6–10.Google Scholar
Thill, D. C., Beck, K. G., and Callihan, R. H. 1984. The biology of downy brome (Bromus tectorum). Weed Sci. 32 (Suppl. 1): 7–12.Google Scholar
Tranel, P. J., Gealy, D. R., and Kennedy, A. C. 1993. Inhibition of downy brome (Bromus tectorum) root growth by a phytotoxin from Pseudomonas fluorescens strain D7. Weed Technol. 7:134–139.Google Scholar
Turgeon, A. J., ed. 1980. Turfgrass species. Pages 41–94 In Turfgrass Management. Reston, VA: Reston Publishing.Google Scholar
Vallentine, J. F., ed. 1971. Noxious plant problems and plant control. Pages 31–70 In Range Development and Improvement. Provo, UT: Brigham Young University Press.Google Scholar
VanPeer, R. and Schippers, B. 1989. Plant growth responses to bacterization with selected Pseudomonas spp. strains and rhizosphere microbial development in hydroponics cultures. Can. J. Microbiol. 35:456–463.Google Scholar
Vogelgsang, S., Watson, A. K., DiTommaso, A., and Hurle, K. 1999. Susceptibility of various accessions of Convolvulus arvensis to Phomopsis convolvulus . Biol. Control 15:25–32.CrossRefGoogle Scholar
Wapshere, A. J. 1974. A strategy for evaluating the safety of organisms for biological weed control. Ann. Appl. Biol. 77:201–211.CrossRefGoogle Scholar
Watrud, L. S., Perlak, F. J., Tran, M. T., Kusano, K., and Meyer, J. 1985. Cloning of the Bacillus thuringiensis subsp. kurstaki delta-endotoxin gene into Pseudomonas fluorescens: molecular biology and ecology of an engineered microbial pesticide. Pages 40–46 In Halvorson, H. O., Palmer, D. and Rogul, M., eds. Engineered Organisms in the Environment: Scientific Issues. Washington: American Society of Microbiologists.Google Scholar
Weller, D. M. 1988. Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annu. Rev. Phytopathol. 26:379–407.Google Scholar
Willis, A. J. 1990. Ecological consequences of modern weed control systems. Pages 501–520 In Hance, R. J. and Holly, K., eds. Weed Control Handbook: Principles. 8th ed. London: Blackwell Scientific.Google Scholar