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Herbicidal Influence on Amylase in Barley and Squash Seedlings

Published online by Cambridge University Press:  12 June 2017

Donald Penner
Donald Penner*
Affiliation:
Department of Crop Science, Michigan State University, East Lansing, Michigan
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Abstract

The development of amylase activity controlled by the embryo in the distal halves of intact barley (Hordeum vulgare L., var. Larker) seeds during the first 2 days of germination was prevented by the presence in the culture solution of 10−4 M 7-oxabicyclo-(2.2.1)heptane-2,3-dicarboxylic acid (endothall), 3,5-dibromo-4-hydroxybenzonitrile (bromoxynil), 2,6-dichlorobenzonitrile (dichlobenil), or 3 amino-2,5-dichlorobenzoic acid (amiben) and was not overcome by the simultaneous addition of gibberellic acid. The aforementioned herbicides did not inhibit gibberellic acid-induced amylase synthesis in de-embryonated halved barley seeds. The amiben and bromoxynil inhibition of barley germination was slightly reduced by the simultaneous addition of 10−2 M glucose to the culture solution. Amiben and bromoxynil also inhibited the development of low levels amylase activity found in 2-day-old squash (Cucurbita maxima Duchesne, var. Chicago Warted Hubbard) cotyledons; however, added benzyladenine overcame the inhibition. In contrast to barley, benzyladenine was shown to induce the development of amylase activity in squash. Tolerance of squash and other seeds low in carbohydrates to amiben and bromoxynil during germination may be related to their independence from herbicide-inhibited carbohydrate degradation for energy and carbon source required for anabolic processes.

Type
Research Article
Information
Weed Science , Volume 16 , Issue 4 , October 1968 , pp. 519 - 522
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

1. Bernfeld, P. 1951. Enzymes of starch degradation and synthesis, p. 379–395. Nord, F. F. (Ed.), Advances in Enzymology.CrossRefGoogle Scholar
2. Black, H. S. and Altschul, A. M. 1965. Gibberellic acid-induced lipase and α-amylase formation and their inhibition by aflatoxin. Biochem. Biophys. Res. Commun. 19:661–664.CrossRefGoogle ScholarPubMed
3. Briggs, D. E. 1963. Biochemistry of barley germination. Action of gibberellic acid on barley endosperm. J. Inst. Brew. 69:13–19.CrossRefGoogle Scholar
4. Chrispeels, M. J. and Varner, J. E. 1966. Inhibition of gibberellic acid induced formation of α-amylase by abscisin II. Nature 212:1066–1067.CrossRefGoogle Scholar
5. Cohen, D. and Paleg, L. G. 1967. Physiological effects of gibberellic acid. X. The release of gibberellin-like substances by germinating barley embryos. Plant Physiol. 42:1288–1296.CrossRefGoogle ScholarPubMed
6. Duffus, J. H. 1967. A cell-free system for the study of α-amylase synthesis in barley aleurone layers. Biochem. J. 103:215–217.CrossRefGoogle Scholar
7. Jacobsen, J. V. and Varner, J. E. 1967. Gibberellic acid-induced synthesis of protease by isolated aluerone layers of barley. Plant Physiol. 42:1596–1600.CrossRefGoogle ScholarPubMed
8. MacLeod, A. M., Duffus, J. H., and Johnston, C. S. 1964. Development of hydrolytic enzymes in germinating grain. J. Inst. Brew. 70:521–528.CrossRefGoogle Scholar
9. Mann, J. E., Cota-Robles, E., Yung, K. H., Pu, M., and Haid, H. 1967. Phenylurethane herbicides: inhibitors of changes in metabolic state. I. Botanic aspects. Biochem. Biophys. Acta 138:133–139.Google Scholar
10. Paleg, L. G. 1960. Physiological effects of gibberellic acid. I. On carbohydrate metabolism and amylase activity of barley endosperm. Plant Physiol. 35:293–299.CrossRefGoogle ScholarPubMed
11. Penner, D. and Ashton, F. M. 1966. Proteolytic enzyme control in squash cotyledons. Nature 212:935–936.CrossRefGoogle ScholarPubMed
12. Penner, D. and Ashton, F. M. 1968. Influence of dichlobenil, endothall, and bromoxynil on kinin control of proteolytic activity. Weed Sci. 16:323–326.CrossRefGoogle Scholar
13. Ram Chandra, G. and Varner, J. E. 1965. Gibberellic acid-controlled metabolism of RNA in aleurone cells of barley. Biochem. Biophys. Acta 108:583–592.Google Scholar
14. Shuster, L. and Gifford, R. H. 1962. Changes in 3′nucleoti-dase during germination of wheat embryos. Arch. Biochem. Biophys. 96:534–540.CrossRefGoogle Scholar
15. Varner, J. E. 1964. Gibberellic acid controlled synthesis of α-amylase in barley endosperm. Plant Physiol. 39:413–415.CrossRefGoogle ScholarPubMed
16. Varner, J. E. and Ram Chandra, G. 1964. Hormonal control of enzyme synthesis in barley endosperm. Proc. Nat. Acad. Sci. U.S. 52:100–106.CrossRefGoogle ScholarPubMed
17. Yung, K. H. and Mann, J. D. 1967. Inhibition of early steps in the gibberellin-activated synthesis of α-amylase. Plant Physiol. 42:195–200.CrossRefGoogle ScholarPubMed