Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-05T01:01:56.600Z Has data issue: false hasContentIssue false
  • English
  • Français

Growth Inhibition and Disruption of Mitosis by DCPA in Oat (Avena sativa) Roots

Published online by Cambridge University Press:  12 June 2017

Jeffrey D. Holmsen  and
F. Dan Hess
Jeffrey D. Holmsen
Affiliation:
Dep. Bot. and Plant Pathol., Purdue Univ., West Lafayette, IN 47907
F. Dan Hess
Affiliation:
Dep. Bot. and Plant Pathol., Purdue Univ., West Lafayette, IN 47907
Get access Rights & Permissions [Opens in a new window]

Abstract

One to 5.6 μM DCPA (dimethyl tetrachloroterephthalate) inhibited oat (Avena sativa L. ‘Victory’) root growth within 12 to 18 h. Treated roots were severely stunted and swollen. An analysis of cell division in roots treated with DCPA revealed a disruption of normal mitosis after prophase. Metaphase, anaphase, and telophase division figures were absent 8 to 10 h after treatment with 5.6 μM DCPA. In contrast, a 24-h treatment with 5.6 μM DCPA was necessary to eliminate prophase division figures. The number of aberrant division figures increased concomitantly with the reduction in normal division figures. The predominant type of aberrant division figure was a condensed prophase. When the aberrant division cycle was completed and cells entered interphase, the dispersed chromosomes coalesced to form large, polymorphic nuclei and, occasionally, micronuclei. Approximately 60% of the outer four tiers of cells in roots treated with 5.6 μM DCPA developed abnormal cell walls. These data suggest that DCPA causes root growth inhibition by disrupting several processes involving organized microtubules.

Keywords

Cell divisioncytokinesiscell platechlorthal dimethyl
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 32 , Issue 6 , November 1984 , pp. 732 - 738
Copyright
Copyright © 1984 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Anderson, J. L. and Shaybany, B. 1972. Effects of DCPA on tomato hypocotyl tissue. Weed Sci. 20:434–439.CrossRefGoogle Scholar
2. Bajer, A. S. and Mole-Bajer, J. 1972. Spindle dynamics and chromosome movements. Int. Rev. Cytol., Suppl. 3:1–271.Google Scholar
3. Barlow, P. W. 1969. Differences in response to colchicine by differentiating xylem cells in roots of Pisum . Protoplasma 68:79–83.CrossRefGoogle ScholarPubMed
4. Bayer, D. E., Foy, C. L., Mallory, T. E., and Cutter, E. G. 1967. Morphological and histological effects of trifluralin on root development. Am. J. Bot. 54:945–952.Google Scholar
5. Bingham, S. W. 1967. Influence of herbicides on root development of bermudagrass. Weeds 15:363–365.Google Scholar
6. Bingham, S. W. 1968. Effect of DCPA on anatomy and cytology of roots. Weed Sci. 16:449–452.CrossRefGoogle Scholar
7. Chang, C. T. and Smith, D. 1972. Effect of DCPA on ultrastructure of foxtail millet cells. Weed Sci. 20:220–225.CrossRefGoogle Scholar
8. Doxey, D. 1949. The effect of isopropyl phenyl carbamate on mitosis in rye (Secale cereale) and onion (Allium cepa). Ann. Bot. (Lond.) 13:329–336.CrossRefGoogle Scholar
9. Ennis, W. B. Jr. 1948. Some cytological effects of O-isopropyl-N-phenyl carbamate upon Avena . Am. J. Bot. 35:15–21.Google Scholar
10. Gunning, B. E. S. and Steer, M. W. 1975. Pages 157–171 in Ultrastructure and the Biology of Plant Cells. Edward Arnold, Ltd., London.Google Scholar
11. Hepler, P. K. and Jackson, W. T. 1969. Isopropyl-N-phenyl carbamate affects spindle microtubule orientation in dividing endosperm cells of Haemanthus katherinae Baker. J. Cell Sci. 5:727–743.Google Scholar
12. Hepler, P. K. and Palevitz, B. A. 1974. Microtubules and microfilaments. Annu. Rev. Plant Physiol. 25:309–362.CrossRefGoogle Scholar
13. Hillmann, G. and Ruthmann, A. 1982. Effect of mitotic inhibitors on the ultrastructure of root meristem cells. Planta 155:124–132.Google Scholar
14. Hoagland, D. R. and Arnon, D. I. 1950. The water culture method for growing plants without soil. Calif. Agric. Exp. Stn. Circ. 347.Google Scholar
15. Humphrey, C. D. and Pittman, F. E. 1974. A simple methylene blue-azure II-basic fuchsin stain for epoxy-embedded tissue sections. Stain Technol. 49:9–14.Google Scholar
16. Jensen, W. A. 1962. Botanical Histochemistry. W. H. Freeman and Co., San Francisco. 408 pp.Google Scholar
17. Jones, M. G. K. and Payne, H. L. 1977. Cytokinesis in Impatiens balsamina and the effect of caffeine. Cytobios 20:79–91.Google Scholar
18. Kihlman, B. A. 1966. Pages 106–110 in Actions of Chemicals on Dividing Cells. Prentice-Hall, Inc., Englewood Cliffs, NJ.Google Scholar
19. Lignowski, E. M. and Scott, E. G. 1971. Trifluralin and root growth. Plant Cell Physiol. 12:701–708.Google Scholar
20. Nishimoto, R. K. and Warren, G. F. 1971. Site of uptake, movement, and activity of DCPA. Weed Sci. 19:152–155.CrossRefGoogle Scholar
21. Nishimoto, R. K. and Warren, G. F. 1971. Stem abnormality induced by DCPA. Weed Sci. 19:343–346.Google Scholar
22. Osgood, R. V. and Romanowski, R. R. Jr. 1967. The phytotoxicity, site of uptake and translocation of DCPA in resistant and susceptible cotyledon-stage weed species. Pages 123–126 in Proc. 1st Asian-Pac. Weed Contr. Interchange.Google Scholar
23. Parker, C. 1966. The importance of shoot entry in the action of herbicides applied to the soil. Weeds 14:117–121.Google Scholar
24. Paul, D. C. and Goff, C. W. 1973. Comparative effects of caffeine, its analogues and calcium deficiency on cytokinesis. Exp. Cell Res. 78:399–413.Google Scholar
25. Pickett-Heaps, J. D. 1967. The effects of colchicine on the ultrastructure of dividing plant cells, xylem wall differentiation and distribution of cytoplasmic microtubules. Dev. Biol. 15:206–236.Google Scholar
26. Scott, M. A. and Struckmeyer, B. E. 1955. Morphology and root anatomy of squash and cucumber seedlings treated with isopropyl N-(3-chlorophenyl) carbamate (CIPC). Bot. Gaz. 117:37–45.Google Scholar
27. Shaybany, B. and Anderson, J. L. 1972. Effect of chlorthal dimethyl on oat and foxtail seedling anatomy. Weed Res. 12:164–168.CrossRefGoogle Scholar
28. Weed Science Society of America. 1983. DCPA. Pages 144–146 in Herbicide Handbook, 5th ed. Weed Sci. Soc. Am., Champaign, IL.Google Scholar