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Glyphosate-Resistant Kochia (Kochia scoparia) in Kansas: EPSPS Gene Copy Number in Relation to Resistance Levels

Published online by Cambridge University Press:  20 January 2017

Amar S. Godar ,
Phillip W. Stahlman ,
Mithila Jugulam  and
J. Anita Dille
Amar S. Godar
Affiliation:
Department of Agronomy, Kansas State University, Manhattan, KS 66506
Phillip W. Stahlman*
Affiliation:
Kansas State University Agricultural Research Center, Hays, KS 67601
Mithila Jugulam
Affiliation:
Department of Agronomy, Kansas State University, Manhattan, KS 66506
J. Anita Dille
Affiliation:
Department of Agronomy, Kansas State University, Manhattan, KS 66506
*
Corresponding author's E-mail: [email protected]
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Abstract

Reports of kochia control failure with glyphosate in western Kansas increased dramatically in the years following confirmed presence of glyphosate-resistant (GR) populations in 2007. In this study, progeny from 8 of 16 geographically dispersed kochia populations in western Kansas (seed collected in 2010) were confirmed to be resistant to glyphosate by conducting whole-plant dose-response (in greenhouse and/or outdoor environments) and shikimate-accumulation assays. Additionally, the relationship between 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene copy number and glyphosate resistance levels was investigated. A known glyphosate-susceptible (GS) kochia population from Ellis County, Kansas was used for comparison in all studies. Based on the herbicide rate that caused 50% reduction in biomass compared to untreated control (GR50) values, the 8 GR kochia populations were 4 to 11 times more resistant to glyphosate compared to the GS population. The GR50 values of kochia populations were 1.58 to 1.85 times higher under an outdoor environment compared to when grown in the greenhouse. Glyphosate-treated leaf discs of the GS kochia plants accumulated consistently higher amounts of shikimate than those of the GR plants. Additionally, the GR plants with higher levels of resistance to glyphosate had higher EPSPS : acetolactate synthase (ALS) relative gene copy number compared to those with low levels of resistance.

Keywords

Glyphosate, kochia, Kochia scoparia (L.) SchradDose responseEPSPS gene amplificationherbicide resistanceshikimate accumulation
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 63 , Issue 3 , September 2015 , pp. 587 - 595
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Anderson, R (1994) Characterizing weed community seedling emergence for a semiarid site in Colorado. Weed Technol. 8:245–249 Google Scholar
Anderson, R, Nielsen, D (1996) Emergence pattern of five weeds in the central Great Plains. Weed Technol. 10:744–749 Google Scholar
Beckie, HJ, Blackshaw, RE, Low, R, Hall, LM, Sauder, CA, Martin, S, Brandt, RN, Shirriff, SW (2013) Glyphosate- and acetolactate synthase inhibitor-resistant kochia (Kochia scoparia) in western Canada. Weed Sci. 61:310–318 Google Scholar
Cranston, HJ, Kern, AJ, Hackett, JL, Miller, EK, Maxwell, BD, Dyer, WE (2001) Dicamba resistance in kochia. Weed Sci. 49:164–170 Google Scholar
Crespo, RJ, Bernards, ML, Sbatella, GM, Kruger, GR, Lee, DJ, Wilson, RG (2014) Response of Nebraska kochia (Kochia scoparia) accessions to dicamba. Weed Technol. 28:151–162 Google Scholar
Dahl, GK, Dexter, AG, Nalewaja, JD (1982) Kochia competition and control in wheat. Pages 15–16 in Proceedings of the 37th Annual Meeting. Indianapolis, IN North Central Weed Science Society Google Scholar
Dexter, AG, Nalewaja, JD, Rasmusson, DD, Buchli, J (1981) Survey of wild oats and other weeds in North Dakota 1978 and 1979. N D Agric Exp Stn Res Rep. 79:1–8 Google Scholar
Dille, JA, Stahlman, PW, Geier, PW, Riffel, J, Currie, RS, Wilson, RG, Sbatella, GM, Westra, P, Kniss, AR, Moechnig, MJ, Cole, RM (2012) Kochia emergence profiles across the Central Great Plains. Abstract# 122 in Proceedings of 2012 Annual Meeting. Lawrence, KS Weed Science Society of America Google Scholar
Durgan, BR, Dexter, AG, Miller, SD (1990) Kochia (Kochia scoparia) interference in sunflower (Helianthus annuus). Weed Technol. 4:52–56 Google Scholar
Durham, RM, Durham, JW (1979) Kochia: its potential for forage production. Pages 444–450 in Goodin JR, Northington DK, eds. Arid Land Plant Resources. Lubbock, TX Texas Tech University Google Scholar
Eberlein, C, Fore, Z (1984) Kochia biology. Weeds Today. 15:5–6 Google Scholar
Everitt, J, Alaniz, M, Lee, J (1983) Seed germination characteristics of Kochia scoparia . J Range Manag. 36:646–648 Google Scholar
Fay, PK, Mulugeta, DM, Dyer, WE (1992) The role of seed dispersal in the spread of sulfonylurea-resistant Kochia scoparia . Pages 49 page in Proceedings of the 32th Annual Meeting. Lawrence, KS Weed Science Society of America Google Scholar
Foes, MJ, Liu, L, Vigue, G, Stoller, EW, Wax, LM, Tranel, PJ (1999) A kochia (Kochia scoparia) biotype resistant to triazine and ALS-inhibiting herbicides. Weed Sci. 47:21–27 Google Scholar
Forcella, F (1985) Final distribution is related to rate of spread in alien weeds. Weed Res. 25:181–191 Google Scholar
Friesen, LF, Beckie, HJ, Warwick, SI, Van Acker, RC (2009) The Biology of Canadian weeds. 138. Kochia scoparia (L.) Schrad. Can J Plant Sci. 89:141–167 Google Scholar
Gaines, TA, Zhang, W, Wang, D, Bukun, B, Chisholm, ST, Shaner, DL, Nissen, SJ, Patzoldt, WL, Tranel, PJ, Culpepper, AS, Grey, TL, Webster, TM, Vencill, WK, Sammons, RD, Jiang, J, Preston, C, Leach, JE, Westra, P (2010) Gene amplification confers glyphosate resistance in Amaranthus palmeri . Proc Natl Acad Sci U S A. 107:1029–1034 Google Scholar
Godar, AS, Stahlman, PW (2015) Consultant's perspective on the evolution and management of glyphosate-resistant kochia (Kochia scoparia) in western Kansas. Weed Technol. 29 318–328 Google Scholar
Heap, I (2014) The International Survey of Herbicide Resistant Weeds. http://www.weedscience.org/Summary/Species.aspx?WeedID=101. Accessed August 27, 2014Google Scholar
Jasieniuk, M, Maxwell, BD (1994) Population genetics and the evolution of herbicide resistance in weeds. Phytoprotection. 75:25–35 Google Scholar
Jugulam, M, Niehues, K, Godar, AS, Koo, DH, Danilova, T, Friebe, B, Sehgal, S, Varanasi, VK, Wiersma, A, Westra, P, Stahlman, PW, Gill, BS (2014) Tandem amplification of a chromosomal segment harboring 5-enolpyruvylshikimate-3-phosphate synthase locus confers glyphosate resistance in Kochia scoparia . Plant Physiol. 166:1200–1207 Google Scholar
Knezevic, SZ, Streibig, JC, Ritz, C (2007) Utilizing R software package for dose-response studies: the concept and data analysis. Weed Technol. 21:840–848 Google Scholar
Kocacinar, F, Sage, R (2003) Photosynthetic pathway alters xylem structure and hydraulic function in herbaceous plants. Plant Cell Environ. 26:2015–2026 Google Scholar
Koger, CH, Shaner, DL, Henry, WB, Nadler-Hassar, T, Thomas, WE, Wilcut, JW (2005) Assessment of two nondestructive assays for detecting glyphosate resistance in horseweed (Conyza canadensis). Weed Sci. 53:438–445 Google Scholar
Kumar, V, Jha, P, Reichard, R (2014) Occurrence and characterization of kochia (Kochia scoparia) accessions with resistance to glyphosate in Montana. Weed Technol. 28:122–130 Google Scholar
Lewis, DW, Gulden, RH (2014) Effect of kochia (Kochia scoparia) interference on sunflower (Helianthus annuus) yield. Weed Sci. 62:158–165 Google Scholar
Mengistu, LW, Messersmith, CG (2002) Genetic diversity of kochia. Weed Sci. 50:498–503 Google Scholar
Mueller, TC, Massey, JH, Hayes, RM, Main, CL, Stewart, CN (2003) Shikimate accumulates in both glyphosate-sensitive and glyphosate-resistant horseweed (Conyza canadensis L. Cronq.). J Agric Food Chem. 51:680–684 Google Scholar
Peterson, DE (1999) The impact of herbicide-resistant weeds on Kansas agriculture. Weed Technol. 13:632–635 Google Scholar
R Core Team. (2014) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org. Accessed July 08, 2014Google Scholar
Ritz, C, Streibig, JC (2005) Bioassay analysis using R. J Stat Softw. 12:1–22 Google Scholar
Ritz, C, Streibig, JC (2014) drc: Analysis of Dose-Response Curve Data. Version 2.3-96. http://cran.r-project.org/web/packages/drc/drc.pdf. Accessed July 08, 2014Google Scholar
Schwinghamer, TD, Van Acker, RC (2008) Emergence timing and persistence of kochia (Kochia scoparia). Weed Sci. 56:37–41 Google Scholar
Seefeldt, SS, Jensen, JE, Fuerst, P (1995) Log-logistic analysis of herbicide dose-response relationship. Weed Technol. 9:218–227 Google Scholar
Shaner, DL, Nadler-Hasser, T, Koger, CH (2005) A rapid in vivo shikimate accumulation assay with excised leaf discs. Weed Sci. 53:769–774 Google Scholar
Simarmata, M, Kaufmann, JE, Penner, D (2003) Potential basis of glyphosate resistance in California rigid ryegrass (Lolium rigidum). Weed Sci. 51:678–682 Google Scholar
Stallings, GP, Thill, DC, Mallory-Smith, CA, Shafii, B (1995) Pollen-mediated gene flow of sulfonylurea-resistant kochia (Kochia scoparia). Weed Sci. 43:95–102 Google Scholar
Thompson, C, Thill, D, Shafi, B (1994) Germination characteristics of sulfonylurea-resistant and -susceptible Kochia (Kochia scoparia). Weed Sci. 42:50–56 Google Scholar
Waite, J, Thompson, CR, Peterson, DE, Currie, RS, Olson, BLS, Stahlman, PW, Al-Khatib, K (2013) Differential kochia (Kochia scoparia) populations response to glyphosate. Weed Sci. 61:193–200 Google Scholar
Wicks, GA, Martin, AR, Haack, AE, Mahnken, GW (1994) Control of triazine-resistant kochia (Kochia scoparia) in sorghum (Sorghum bicolor). Weed Technol. 8:748–753 Google Scholar
Wiersma, A (2012) Regional whole plant and molecular response of Kochia scoparia to glyphosate. . Fort Collins, CO Colorado State University. 63 pGoogle Scholar