Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-26T05:18:52.343Z Has data issue: false hasContentIssue false
  • English
  • Français

Glyphosate Does Not Inhibit Photosynthetic Electron Transport and Phosphorylation in Pea (Pisum sativum) Chloroplasts

Published online by Cambridge University Press:  12 June 2017

E. P. Richard Jr. ,
J. R. Goss  and
C. J. Arntzen
E. P. Richard Jr.
Affiliation:
Dep. Agron., Univ. of Illinois
J. R. Goss
Affiliation:
Dep. Agron., Univ. of Illinois
C. J. Arntzen
Affiliation:
U. S. Dep. Agric., Sci. Ed. Admin., and Dep. Botany, Univ. of Illinois, Urbana, IL 61801
Get access Rights & Permissions [Opens in a new window]

Abstract

The activity of glyphosate [N-(phosphonomethyl)glycine], formulated as the isopropylamine salt, on in vitro photosynthesis was investigated. When pH 4.7 glyphosate solutions were titrated to a pH equal to that of the reaction media (pH 7.8), glyphosate additions had no effect on whole chain electron transport between coupled photosystem II (PS II) and photosystem I (PS I) in stroma-free chloroplast thylakoids from peas (Pisum sativum L. ‘Morse's Progress No. 9′). Inhibition did not occur even after a 2-h dark incubation of lamellae in a 5-mM solution of glyphosate. Fluorescence studies failed to detect an effect of glyphosate on PS-II mediated electron transport processes or upon light harvesting properties of PS II even after a 2-h glyphosate/chloroplast preincubation. Glyphosate had no effect on cyclic and noncyclic photophosphorylation even after a 100-min dark incubation of chloroplast membranes in a 5-mM solution of glyphosate. Based on these assays it is concluded that glyphosate has no direct effect on the photochemical reactions of photosynthesis when the pH of the active compound is adjusted to that of the reaction mixture prior to addition to a chloroplast suspension.

Keywords

Mehler reactionHill reactionfluorescencepH adjustment
Type
Research Article
Information
Weed Science , Volume 27 , Issue 6 , November 1979 , pp. 684 - 688
Copyright
Copyright © 1979 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Arnon, D. J. 1949. Copper enzymes in isolated chloroplasts, polyphenoloxidase in Beta vulgaris L. Plant Physiol. 24:1–15.Google Scholar
2. Arntzen, C. J. 1978. Dynamic structural features of chloroplast lamellae. Curr. Top. Bioenerg. 8:111–160.Google Scholar
3. Avron, M. 1977. Energy translocation in chloroplasts. Annu. Rev. Biochem. 46:143–155.Google Scholar
4. Baird, D. D., Upchurch, R. P., Homesley, W. B., and Franz, J. E. 1971. Introduction of a new broad spectrum postemergence herbicide class with utility for herbaceous perennial weed control. Proc. North Cent. Weed Control Conf. 26:64–68.Google Scholar
5. Barber, J. 1976. Ionic regulation in intact chloroplasts and its effect on primary photosynthetic processes. Pages 89–134 in Barber, J. The Intact Chloroplast. Elsevier Scientific Pub. Co. Amsterdam.Google Scholar
6. Boyle, W. S. and Evans, J. O. 1974. Effect of glyphosate and ethephon on meiotic chromosomes. J. Hered. 65:250.Google Scholar
7. Brecke, B. J. and Duke, W. B. 1977. Effect of glyphosate on intact bean plants, leaf discs, and isolated cells. Abstr. Weed Sci. Soc. Am. pp. 87–88.Google Scholar
8. Burke, J. J., Ditto, C. L., and Arntzen, C. J. 1978. Involvement of the light-harvesting complex in cation regulation of excitation energy distribution in chloroplasts. Arch. Biochem. Biophys. 187:252–263.Google Scholar
9. Campbell, W. F., Evans, J. O., and Reed, S. C. 1976. Effects of glyphosate on chloroplast ultrastructure of quackgrass mesophyll cells. Weed Sci. 24:22–25.Google Scholar
10. Croft, S. M., Arntzen, C. J., Vanderhoff, L. N., and Zettinger, C. S. 1974. Inhibition of chloroplast ribosome formation by N, N-bis (phosphonomethyl)glycine. Biochim. Biophys. Acta 335:211–217.Google Scholar
11. Duke, S. O. and Hoagland, R. E. 1978. Effects of glyphosate on metabolism of phenolic compounds I. Induction of phenylalanine ammonia-lyase activity in dark-grown maize roots. Plant Sci. Lett. 11:185–190.Google Scholar
12. Good, N. E., Izawa, S., and Hind, G. 1966. Uncoupling and energy transfer inhibition in photophosphorylation. Curr. Top. Bioenerg. 1:75–112.CrossRefGoogle Scholar
13. Hoagland, R. E. and Paul, R. 1978. Ultrastructural effects of glyphosate on Lemma gibba L. Abstr. Weed Sci. Soc. Am. p. 78.Google Scholar
14. Hoagland, R. E., Duke, S. O., and Elmore, D. 1978. Effects of glyphosate on metabolism of phenolic compounds. II. Influence of soluble hydroxyphenolic compound, free amino acid, and soluble protein levels in dark-grown maize roots. Plant Sci. Lett. 13:291–299.Google Scholar
15. Jaworski, E. G. 1972. Mode of action of N-phosphonomethylglycine: Inhibition of aromatic amino acid biosynthesis. J. Agric. Food Chem. 20:1195–1198.Google Scholar
16. Nishimura, M. T. I. and Chance, B. 1962. Studies of bacterial photophosphorylation. III. A sensitive and rapid method of determination of phosphorylation. Biochim. Biophys. Acta. 59:177–82.Google Scholar
17. Papageorgiou, G. 1975. Chlorophyll fluorescence: an intrinsic probe of photosynthesis. Pages 320–373 in Govindjee, , ed. Bioenergetics of Photosynthesis. Academic Press, New York.Google Scholar
18. Sprankle, P., Meggitt, W. F., and Penner, D. 1975. Absorption, mobility and microbial degradation of glyphosate in the soil. Weed Sci. 23:229–234.Google Scholar
19. Sprankle, P., Meggitt, W. F., and Penner, D. 1975. Absorption, action and translocation of glyphosate. Weed Sci. 23:235–240.Google Scholar
20. Van Rensen, J. J. S. 1974. Effects of N-(phosphonomethyl)glycine on photosynthetic reactions in Scenedesmus and in isolated spinach chloroplasts. Pages 683–687 in Avron, M., ed. Proceedings of the Third International Congress on Photosynthesis. Elsevier Scientific Pub. Co., Amsterdam.Google Scholar