Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-23T06:07:28.523Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetics of Resistance to Acetohydroxyacid Synthase Inhibitors in Populations of Eastern Black Nightshade (Solanum ptychanthum) from Ontario

Published online by Cambridge University Press:  20 January 2017

Jamshid Ashigh ,
Istvan Rajcan  and
François J. Tardif
Jamshid Ashigh
Affiliation:
Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada. N1G 2W1
Istvan Rajcan
Affiliation:
Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada. N1G 2W1
François J. Tardif*
Affiliation:
Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada. N1G 2W1
*
Corresponding author's E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Resistance to acetohydroxyacid synthase (AHAS) inhibiting herbicides in populations of eastern black nightshade from Ontario has been linked to an Ala205Val substitution in the AHAS enzyme. The aim of this study was to determine the mechanism of inheritance of AHAS inhibitor resistance and the genetic relationships among resistant (R) and susceptible (S) eastern black nightshade populations from Ontario. Homozygous R and S parental populations were crossed and the inheritance was analyzed in F1 (S × R), reciprocal F1 (R × S), F2, and backcross (S × F1) progenies after application of imazethapyr at 150 g ai ha−1. Compared to parental lines, the progenies were rated as R, intermediate (I), and S phenotypes. All the F1 progenies were of the I phenotype. The backcross progenies segregated in a 1:1 (S:I) ratio, and the F2 families segregated in a 1:2:1 (R:I:S) ratio. These results indicate that a single nuclear gene, with incomplete dominance, controls resistance to AHAS-inhibiting herbicides in R population of eastern black nightshade. Random amplified polymorphic DNA (RAPD) markers were screened among 25 R and S populations. The genetic relationship of R and S populations based on RAPD profiles generated from six RAPD primers indicated four groups of populations in which resistance seems to have arisen independently. However, based on similarity coefficients, resistance within three of the groups could have arisen by gene flow. Both similar local selection pressure and gene flow could explain the spread of the Ala205Val substitution in R populations of eastern black nightshade in Ontario.

Keywords

Imazethapyreastern black nightshade, Solanum ptychanthum Dun. SOLPTAcetolactate synthaseALSRAPDherbicide resistanceinheritancegenetic diversity
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 56 , Issue 2 , April 2008 , pp. 210 - 215
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Alex, J. F. 1964. Weeds of tomato and corn fields in two regions of Ontario. Weed Res. 4:308–318.Google Scholar
Anonymous 2007. North American Migration Flyways. http://www.birdnature.com/flyways.html. Accessed: August 2, 2007.Google Scholar
Ashigh, J. 2006. Resistance to acetohydroxyacid synthase-inhibiting herbicides in populations of eastern black nightshade (Solanum ptycanthum Dun.) from Ontario. Guelph, ON, Canada University of Guelph. . 198. p.Google Scholar
Ashigh, J. and Tardif, F. J. 2006. ALS-inhibitor resistance in populations of eastern black nightshade (Solanum ptycanthum) from Ontario. Weed Technol. 20:308–314.CrossRefGoogle Scholar
Ashigh, J. and Tardif, F. J. 2007. An Ala205Val substitution in acetohydroxyacid synthase of eastern black nightshade (Solanum ptychanthum) reduces sensitivity to herbicides and feedback inhibition. Weed Sci. 55:558–565.CrossRefGoogle Scholar
Bassett, I. J. and Munro, D. B. 1985. The biology of Canadian weeds. 67. Solanum ptycanthum Dun., S. nigrum L. and S. sarrachoides Sendt. Can. J. Plant Sci. 65:401–414.Google Scholar
Boutsalis, P. and Powles, S. B. 1995. Inheritance and mechanism of resistance to herbicides inhibiting acetolactate synthase in Sonchus oleraceus L. Theor. Appl. Genet. 91:242–247.Google Scholar
Bowley, S. R. 1999. A Hitchhiker's Guide to Statistics in Plant Biology. Guelph, ON Plants et al., Inc. 250. p.Google Scholar
Dawson, I. K., Simons, A. J., Waugh, R., and Powell, W. 1995. Diversity and genetic differentiation among subpopulations of Gliricidia sepium revealed by PCR-based assays. Heredity. 74:10–18.Google Scholar
Heap, I. 2007. International Survey of Herbicide Resistant Weeds. http://www.weedscience.org. Accessed: June 2007.Google Scholar
Hermanutz, L. 1991. Outcrossing in the weed Solanum ptycanthum (Solanaceae): a comparison of agrestal and ruderal populations. Am. J. Bot. 78:638–646.Google Scholar
Jasieniuk, M., Brulé-Babel, A. L., and Morrison, I. N. 1996. The evolution and genetics of herbicide resistance in weeds. Weed Sci. 44:176–193.CrossRefGoogle Scholar
Kolkman, J. M., Slabaugh, M. B., Bruniard, J. M., Berry, S., Bushman, B. S., Olungu, C., Maes, N., Abratti, G., Zambelli, A., Miller, J. F., Leon, A., and Knapp, S. J. 2004. Acetohydroxyacid synthase mutations conferring resistance to imidazolinone or sulfonylurea herbicides in sunflower. Theor. Appl. Genet. 109:1147–1159.Google Scholar
Levin, D. A. 1981. Dispersal versus gene flow in plants. Ann. Mo. Botanical Garden. 68:233–253.Google Scholar
Levin, D. A. and Kerster, H. W. 1974. Gene flow in seed plants. Evol. Biol. 7:139–220.Google Scholar
Mallory-Smith, C. A., Thill, D. E., and Dial, M. J. 1990. Identification of sulfonylurea herbicide-resistant prickly lettuce (Lactuca serriola). Weed Technol. 4:163–168.Google Scholar
Maxwell, B. D. and Mortimer, A. M. 1994. Selection of herbicide resistance. Pages 1–27. in Powles, S. B. and Holtum, J. A. M. Herbicide Resistance in Plants, Biology and Biochemistry. Boca Raton, FL Lewis.Google Scholar
Milliman, L. D., Riechers, D. E., Wax, L. M., and Simmons, F. W. 2003. Characterization of two biotypes of imidazolinone-resistant eastern black nightshade (Solanum ptycanthum). Weed Sci. 51:139–144.Google Scholar
Moodie, M., Finch, R. P., and Marshall, G. 1997. Analysis of genetic variation in wild mustard (Sinapis arvensis) using molecular markers. Weed Sci. 45:102–107.Google Scholar
Ohmes, G. A. Jr. and Kendig, J. A. 1999. Inheritance of an ALS-cross resistant common cocklebur (Xanthium strumarium) biotype. Weed Technol. 13:100–103.CrossRefGoogle Scholar
Rohlf, F. J. 1997. NTSYS-PC 2.02. Numerical taxonomy and multivariate analysis system. Setauket, NY Exeter Software.Google Scholar
Saari, L. L., Cotterman, J. C., and Thill, D. C. 1994. Resistance to acetolactate synthase inhibiting herbicides. Pages 141–170. in Powles, S. B. and Holtum, J. A. M. Herbicide Resistance in Plants, Biology and Biochemistry. Boca Raton, FL Lewis.Google Scholar
Santel, H. J., Bowden, B. A., Sorensen, V. M., and Mueller, K. H. 1999. Flucarbazone-sodium—a new herbicide for the selective control of wild oat and green foxtail in wheat. Pages 23–28. in. Proc. 1999 Brighton Conference – Weeds, Vol. 1. Farnham, UK British Crop Protection Council.Google Scholar
Schilling, E. E. 1978. A systematic study of the Solanum nigrum complex in North America. Bloomington, IN Indiana University. . 123. p.Google Scholar
Stankiewicz, M., Gadamski, G., and Gawronski, S. W. 2001. Genetic variation and phylogenetic relationships of triazine-resistant and triazine susceptible biotypes of Solanum nigrum—analysis using RAPD markers. Weed Res. 41:287–300.Google Scholar
Tranel, P. J. and Wright, T. R. 2002. Resistance of weeds to ALS-inhibiting herbicides: what have we learned. Weed Sci. 50:700–712.Google Scholar
Volenberg, D. S. and Stoltenberg, D. E. 2002. Inheritance of resistance in eastern black nightshade (Solanum ptycanthum) to acetolactate synthase inhibitors. Weed Sci. 50:731–736.Google Scholar
Volenberg, D. S., Stoltenberg, D. E., and Boerboom, C. M. 2000. Solanum ptycanthum resistance to acetolactate synthase inhibitors. Weed Sci. 48:399–401.Google Scholar
Ward, K. I. and Weaver, S. E. 1996. Response of eastern black nightshade (Solanum ptycanthum) to low rates of imazethapyr and metolachlor. Weed Sci. 44:897–902.Google Scholar
Whaley, C. M., Wilson, H. P., and Westwood, J. H. 2007. A new mutation in plant ALS confers resistance to five classes of ALS-inhibiting herbicides. Weed Sci. 55:83–90.Google Scholar
Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, A., and Tingey, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531–6535.Google Scholar
Young, B. C., Nolte, S. A., and Martin, J. R. 2002. Occurrence and management of eastern black nightshade with perennial characteristics. Proc. North Cent. Weed Sci. Soc. 57:32.Google Scholar